Penicillinase-producing strains of Neisseria gonorrhoeae isolated in Rotterdam.

Blog, F B; Chang, Anne B.; Koning, G A de; Oranje, Arnold P.; E, Stolze; Bosscher-Koestsier, G; Jonge-Suy, M P de; Michel, M. F.; O'niel, E.; Ameyden, S de Weerdt-van; Goastra, L

doi:10.1136/sti.53.2.98

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…We have observed the same phenomenon with two transformants carrying the 4.5-Md plasmid and two carrying 3.3-Md plasmids. These plasmids were found to be very stable in their original gonococcal hosts: penicillinase-nonproducing gonococci were found at a frequency of less than 2% after 5 PPNG strains in Table 1) we obtained PPNG and non-PPNG strains originating from the same sample of a single patient. The PPNG strains from these cases showed the same high in vitro stability as strains from patients with PPNGs only.…”

Section: Resultsmentioning

confidence: 99%

“…A significant proportion of PPNG infections encountered in several Western countries seems to have been acquired from foreign sources rather than from local contacts (1). Importation has been mainly from countries in the far east and from the west coast of Africa (1,2,5,18,20,21,27). The origin of these PPNG strains was correlated with two different types of penicillinresistance plasmids: a 4.4-megadalton (Md) plasmid originating in the far east countries and a 3.2-Md plasmid originating in west Africa (9,20,24,25).…”

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confidence: 99%

“…The emergence of PPNG strains at the end of 1976 (5) and their potential dissemination among the general population is of public health concem in the Netherlands. For this reason, the epidemiology of PPNG strains is studied centrally, and all PPNG strains isolated are asked to be sent to the National Institute of Public Health for confirmation of penicillinase production.…”

mentioning

confidence: 99%

“…Two penicillinase-producing strains of Haemophilus parainfluenzae isolated were found to carry a 3.3-Md plasmid species which was indistinguishable from the 3.3-Md gonococcal resistance plasmids. No plasmid deoxyribonucleic acid was found in two strains of penicillinase-producing Branhamella catarrhalis, and these strains produced a penicillinase different from the TEM-1 enzyme.Penicillinase-producing Neisseria gonorrhoeae (PPNG) strains were isolated for the first time in 1976 in the United Kingdom and the United States (2, 21), and since then they have been found in many other countries (4,5,20,22,35). A significant proportion of PPNG infections encountered in several Western countries seems to have been acquired from foreign sources rather than from local contacts (1).…”

mentioning

confidence: 99%

“…Penicillinase-producing Neisseria gonorrhoeae (PPNG) strains were isolated for the first time in 1976 in the United Kingdom and the United States (2,21), and since then they have been found in many other countries (4,5,20,22,35). A significant proportion of PPNG infections encountered in several Western countries seems to have been acquired from foreign sources rather than from local contacts (1).…”

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Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: epidemiology and genetic and molecular characterization of their plasmids

Embden¹,

Klingeren²,

Dessens-Kroon³

et al. 1980

Antimicrob Agents Chemother

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Penicillinase-producing Neisseria gonorrhoeae strains were isolated in the Netherlands with increasing frequency during the period of 1976 to 1979. About 3% of the gonococci isolated in the first half of 1979 produced penicillinase. In contrast to the period of 1976 to 1977, most penicillinase-producing N. gonorrhoeae infections during the period of 1978 to 1979 were contracted in the Netherlands. The results of genetic and molecular studies on 80 penicillinaseproducing N. gonorrhoeae strains were similar to earlier observations of others: resistance plasmids of only two sizes, 4.5 and 3.3 megadaltons (Md), occurred in penicillinase-producing N. gonorrhoeae strains, and these encoded for the TEM-1 enzyme. The 4.5-Md plasmid could be transferred to Escherichia coli when it coexisted with a plasmid of 24 Md. The latter plasmid was present in the vast majority of the strains carrying the 4.5-Md plasmid. One strain carried a cryptic 7.5-Md plasmid in addition to the commonly found 2.5-Md plasmid. Two penicillinase-producing strains of Haemophilus parainfluenzae isolated were found to carry a 3.3-Md plasmid species which was indistinguishable from the 3.3-Md gonococcal resistance plasmids. No plasmid deoxyribonucleic acid was found in two strains of penicillinase-producing Branhamella catarrhalis, and these strains produced a penicillinase different from the TEM-1 enzyme.Penicillinase-producing Neisseria gonorrhoeae (PPNG) strains were isolated for the first time in 1976 in the United Kingdom and the United States (2, 21), and since then they have been found in many other countries (4,5,20,22,35). A significant proportion of PPNG infections encountered in several Western countries seems to have been acquired from foreign sources rather than from local contacts (1). Importation has been mainly from countries in the far east and from the west coast of Africa (1,2,5,18,20,21,27). The origin of these PPNG strains was correlated with two different types of penicillinresistance plasmids: a 4.4-megadalton (Md) plasmid originating in the far east countries and a 3.2-Md plasmid originating in west Africa (9,20,24,25).The emergence of PPNG strains at the end of 1976 (5) and their potential dissemination among the general population is of public health concem in the Netherlands. For this reason, the epidemiology of PPNG strains is studied centrally, and all PPNG strains isolated are asked to be sent to the National Institute of Public Health for confirmation of penicillinase production. Furthermore, the miniimal inhibitory concentrations of various antibiotics are determined for all PPNG strains isolated.To monitor changes at the genetic level, plasmids have been isolated and characterized from about 30% of all PPNG strains isolated in the period 1976 to the beginning of 1979. In this report we describe the results of the epidemiological data, sensitivity testing, and plasmid properties of PPNG strains. Furthermore, several isolates of penicillinase-producing strains of

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: epidemiology and genetic and molecular characterization of their plasmids

Embden¹,

Klingeren²,

Dessens-Kroon³

et al. 1980

Antimicrob Agents Chemother

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Penicillinase-Producing Neisseria gonorrhoeae: Results of Surveillance in the United States

Siegel¹,

Thornsberry²,

Biddle³

et al. 1978

Journal of Infectious Diseases

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In September 1976 the Center for Disease Control (Atlanta, Georgia) initiated surveillance for cases of infection due to penicillinase-productin Neisseria gonorrhoeae in the United States. One hundred ninety-one cases of penicillinase-producing gonococcal infection were confirmed through June 30, 1977. Of 177 cases for which histories of sexual exposure were available, 69 were traced to sexual contact in the Far East, and one was traced to sexual contact in West Africa. Of 181 cases for which clinical information was available, 174 were uncomplicated anogenital infections, but local complications, such as salpingitis, epididymitis, and a Bartholin's gland abscess, were reported. Spectinomycin is the only drug now recommended by the U.S. Public Health Service for treatment of gonorrhea that is effective against uncomplicated infections due to penicillinase-producing gonococci. For these organisms, the distribution of minimal inhibitory concentrations (MICs) of penicillin, ampicillin, tetracycline, erythromycin, and spectinomycin was higher than and significantly different from the distribution of MICs for isolates of non-penicillinase-producing gonococci collected in this country. Since surveillance began the incidence of confirmed cases of penicillinase-producing gonococcal infection has decreased from 20.8 cases per month in 1976 to 16.8 cases per month in 1977.

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Culture Medium for Confirmation of Penicillin-Resistant and Penicillinase-Producing Neisseria gonorrhoeae

1982

J Clin Microbiol

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A culture method for the isolation and identification of penicillinase (1lactamase)-producing Neisseria gonorrhoeae (PPNG) was evaluated in the Philippines where PPNG are common. The method uses plastic biplates containing standard Martin-Lewis gonorrhea culture medium in one side of the biplate and PPNG-selective medium containing 1.5 ,ug of penicillin G per ml and a suspension of Sarcina lutea (Micrococcus lutea) that was susceptible to 0.01 p.g of penicillin G per ml in the other side. Penicillin-resistant gonococci grow on both sides of the biplate. The hydrolysis of penicillin by ,B-lactamase permits the growth of S. lutea around PPNG colonies. With this medium we successfully identified 11 of 12 PPNG strains growing on primary isolation plates. A 48to 72-h incubation period was needed, however, for visible growth of S. lutea around PPNG colonies. A unique advantage of this method was the identification of non-PPNG strains which also grew on penicillin-containing medium but did not allow growth of S. lutea. These relatively penicillin-resistant strains were the cause of infections which were not cured by penicillin treatment in 2 of 11 patients.

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Penicillinase-producing strains of Neisseria gonorrhoeae isolated in Rotterdam.

Cited by 6 publications

References 7 publications

Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: epidemiology and genetic and molecular characterization of their plasmids

Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: epidemiology and genetic and molecular characterization of their plasmids

Penicillinase-Producing Neisseria gonorrhoeae: Results of Surveillance in the United States

Culture Medium for Confirmation of Penicillin-Resistant and Penicillinase-Producing Neisseria gonorrhoeae

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