Penicillinase Production and Intrinsic Resistance to Penicillins in Staphylococcus Aureus

Dyke, K. G. H.; Jevons, M. P.; Parker, M. T.

doi:10.1016/s0140-6736(66)90182-6

Cited by 136 publications

(57 citation statements)

References 15 publications

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“…Penicillinase production by single colonies could be detected by their reaction on starch agar plates according to Dyke, Jevons & Parker (1966). Without the inducer methicillin in the agar, inducible and constitutive enzyme production could be distinguished.…”

Section: Strains Of Staphylococcus Aureusmentioning

confidence: 99%

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Compatibility Tests between Genetic Linkage Groups in a Strain of Staphylococcus aureus

Dornbusch¹

1973

Journal of General Microbiology

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Section: Strains Of Staphylococcus Aureusmentioning

confidence: 99%

“…The activity of the preparation increased with increasing quantities of antiserum. Strain DO 524 which produces A-type penicillinase (Dyke, Jevons & Parker, 1966) was included as a positive control.…”

Section: Compatibility Testsmentioning

confidence: 99%

Compatibility Tests between Genetic Linkage Groups in a Strain of Staphylococcus aureus

Dornbusch¹

1973

Journal of General Microbiology

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“…In addition, the minimum inhibitory concentrations (MICs) of antibiotics and metal ions were determined for a proportion of the isolates by a plate incorporation method. Penicillinase production was detected by the starch-iodine method of Dyke, Jevons & Parker (1966).…”

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confidence: 99%

Linkage of Fusidic Acid Resistance to the Penicillinase Plasmid in Staphylococcus aureus

Lacey

Grinsted

1972

Journal of General Microbiology

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S U M M A R YIn four out of five strains of Staphylococcus aureus examined, genes determining fusidic acid resistance were associated with a penicillinase plasmid. In one strain (FAR 5), although the linkage was unstable, resistance to fusidic acid was co-transduced with resistance to cadmium ions and penicillinase production. Further evidence for a single plasmid in this strain was obtained by studying the physical nature of the plasmid DNA. The molecular weight of the entire linkage group was about I 6 million daltons; the molecular weight of the residual plasmid after loss of fusidic acid resistance was about r 5 million.

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“…Penicillinase-producing colonies were detected on starch plates according to Dyke et al (1966), except that methicillin was omitted.…”

Section: Staphylococcusmentioning

confidence: 99%

Unusual Properties Of Staphylococcus Aureus Strains of the New Epidemic Phage Type-95

Rosdahl

Rosendal

1985

Journal of Medical Microbiology

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SUMMARY. Three hundred and seventy-one penicillin-resistant Staphylococcus aureus strains of phage type 95 isolated in the years [1977][1978][1979][1980][1981][1982][1983] were investigated. They had characteristic resistance patterns to cadmium (Cd), arsenate (As) and mercury (Hg). Most were susceptible to all three heavy metals; 25 strains were resistant to As only and one to Cd and As. The susceptible strains had a uniform medium level of penicillinase production, whereas the As-resistant strains produced large amounts of penicillinase. In most of the strains, penicillin resistance was located on a very unstable penicillinase plasmid. The combinaton of rare properties found in Danish type-95 strains seems to point to the spread of one or two clones. Co-reactions with other phage groups or complexes and results of lysogenisation experiments suggest that the Danish type-95 strains are derived from strains of the 52, 52A, 80, 81 complex. INTRODUCTIONWhen staphylococci of the 52, 52A, 80, 81 and 83A phage-type complexes appeared, it was possible to designate probable ancestor(s) by demonstrating evolutionary processes that might have operated (Asheshov and Winkler, 1966;Jevons et al., 1966). The results of these investigations led to the assumption that strains of phage type 80/81 might belong to one clone (Asheshov and Winkler, 1966), whereas members of the 83A complexes might have different ancestors .In recent years, strains of phage type 95 have spread in Denmark. Among Staphylococcus aureus isolates from cases of bacteraemia, the occurrence increased from I % in 1972 to 9% in I98 I (Rosendal et al., I983), and among the 20 000 strains phage-typed in Denmark in 1983,12% were of phage type 95. The present investigation considers the one-clone theory in relation to type-95 strains spreading in Denmark. A total of 3 108 strains isolated from cases of bacteraemia from 1977-1 982 were surveyed for loss of penicillin resistance in the period between primary isolation and the second testing for antibiotic susceptibility (table I, part 1). From this material, 46 strains were chosen for further investigation of the stability of the penicillinase property (table I, part 2). MATERIALS AND METHODS StaphylococcusFive penicillin-resistant strains, all isolated before 1977, with heavy-metal resistance to arsenate only, were examined quantitatively for penicillinase production. Two of them belonged to the 83A complex and one each to groups I, I1 and 111.The relationship between strains of phage type 95 and other strains was studied in 2586 strains isolated from cases of bacteraemia during 1977-1 98 I . The 2586 strains included penicillin-resistant and penicillin-susceptible strains, but strains lysed by phage 95 only (at routine test dilution; RTD) were omitted. The 2586 strains were surveyed for sensitivity to typing phage 95; a similar survey was made of all 31 1 strains of the 52, 52A, 80, 81 complex isolated from [1970][1971][1972][1973][1974][1975][1976].Phage typing was performed according to the methods of Blair and ...

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Penicillinase Production and Intrinsic Resistance to Penicillins in Staphylococcus Aureus

Cited by 136 publications

References 15 publications

Compatibility Tests between Genetic Linkage Groups in a Strain of Staphylococcus aureus

Compatibility Tests between Genetic Linkage Groups in a Strain of Staphylococcus aureus

Linkage of Fusidic Acid Resistance to the Penicillinase Plasmid in Staphylococcus aureus

Unusual Properties Of Staphylococcus Aureus Strains of the New Epidemic Phage Type-95

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