Penicillium chrysogenum immobilised silica: flame atomic absorption spectrometric Pb determination in industrial effluent, sludge and food samples

Abstract

A solid phase extraction method based on the immobilisation of Penicillium chrysogenum on silica was developed for preconcentration and flame atomic absorption spectrometric determination of Pb(II). The immobilised sorbent was packed in a column to optimise the conditions for its quantitative sorption and desorption. Various parameters such as pH, type of eluent, sample and eluent flow rate and influence of others ions were systematically studied. Pb(II) was quantitatively sorbed at pH 4.0 at a flow rate of 2.0 mL min -1 and eluted with 10.0 mL of 1.0 M HCl at 0.2 mL min -1 flow rate. The devised procedure applied for Pb determination in tap water, an industrial effluent and sludge sample, black tea, spinach leaves and green beans (canned food) was reproducible, with a relative standard deviation of 0.8%. The method was validated by a BCR 701 standard lake sediment sample. The limit of detection and limit of quantification for Pb(II) were 7.16 and 23.88 lg L -1 , respectively, with an enrichment factor of 100.

Application of Waste Egg Shell for Adsorption of Cd(II) and Pb(II) Ions to Protect Environment: Equilibrium, Kinetic and Adsorption Studies

“…Adsorption of M(II) on calcinated egg shell was conducted in batch mode. The glass column (10 mm diameter x 150 mm length) having about 15 to 20 mm glass wool bed was used as filtration media 48 . At optimum adsorption conditions, the concentrations of metal ions in filtrate were measured by anodic stripping voltammeter (Metrohm 797 VA Computrace-Switzerland).…”