Penicillium oxalicum PoFlbC regulates fungal asexual development and is important for cellulase gene expression

Yao, Guangshan; Li, Zhonghai; Wu, Ruimei; Qin, Yuqi; Liu, Guodong; Qu, Yinbo

doi:10.1016/j.fgb.2015.12.012

Cited by 27 publications

(24 citation statements)

References 60 publications

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“…The functional roles associated with members of this family are extraordinarily diverse and include DNA recognition, transcription, mRNA trafficking, cytoskeleton organization, epithelial development, chromatin remodeling, and zinc sensing, amongst others (Laity et al, 2001). In P. oxalicum 114-2, a C2H2 transcription factor FlbC (PDE_08372) regulates fungal asexual development and acts as an essential activator of genes encoding cellulases, hemicellulases, and other proteins with functions in lignocellulose degradation (Yao et al, 2016). Another C2H2 transcription factor BrlA (PDE_00087) has not only a key role in regulating conidiation, but it also regulates secondary metabolism extensively as well as the expression of cellulase genes (Qin et al, 2013).…”

Section: The Repertoire Of Tfs Comprises 37 Familiesmentioning

confidence: 99%

Gene Regulatory Networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 Inferred by a Computational Biology Approach

et al. 2020

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Section: The Repertoire Of Tfs Comprises 37 Familiesmentioning

confidence: 99%

Gene Regulatory Networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 Inferred by a Computational Biology Approach

et al. 2020

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“…Studies on such TFs mainly include the transcription activators CLR-2/ClrB of Aspergillus nidulans FGSC A4 (12) and Penicillium oxalicum 114-2 (13) and HP7-1 (14), XYR1/XLR-1/XlnR of Trichoderma reesei QM9136 (15) and P. oxalicum 114-2 (13), AmyR of P. oxalicum 114-2 (13) and Aspergillus niger CICC2462 (11), and LaeA of P. oxalicum 114-2 (16) and T. reesei QM9414 (17) and the carbon catabolite repressor CreA/CRE1/CRE-1 of A. nidulans FGSC A4 (18) and T. reesei QM9414 (19). Interestingly, expression of genes encoding plant biomass-degrading enzymes and genes involved in asexual reproduction is coregulated by some TFs, such as BrlA (10), ClrC (20), and FlbC (21) of P. oxalicum 114-2.…”

mentioning

confidence: 99%

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

Zhao

Wang

et al. 2018

Appl Environ Microbiol

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Soil fungi produce a wide range of chemical compounds and enzymes with potential for applications in medicine and biotechnology. Cellular processes in soil fungi are highly dependent on the regulation under environmentally induced stress, but most of the underlying mechanisms remain unclear. Previous work identified a key GATA-type transcription factor, NsdD (PoxNsdD; also called POX08415), that regulates the expression of cellulase and xylanase genes in PoxNsdD shares 57 to 64% identity with the key activator NsdD, involved in asexual development in In the present study, the regulatory roles of PoxNsdD in were further explored. Comparative transcriptomic profiling revealed that PoxNsdD regulates major genes involved in starch, cellulose, and hemicellulose degradation, as well as conidiation and pigment biosynthesis. Subsequent experiments confirmed that a Δ strain lost 43.9 to 78.8% of starch-digesting enzyme activity when grown on soluble corn starch, and it produced 54.9 to 146.0% more conidia than the Δ parental strain. During cultivation, Δ cultures changed color, from pale orange to brick red, while the Δ cultures remained bluish white. Real-time quantitative reverse transcription-PCR showed that dynamically regulated the expression of a glucoamylase gene (/), an α-amylase gene (/), and a regulatory gene (/), as well as a polyketide synthase gene (//) for yellow pigment biosynthesis and a conidiation-regulated gene (/). Moreover, binding experiments showed that PoxNsdD bound the promoter regions of the above-described genes. This work provides novel insights into the regulatory mechanisms of fungal cellular processes and may assist in genetic engineering of for potential industrial and medical applications. Most filamentous fungi produce a vast number of extracellular enzymes that are used commercially for biorefineries of plant biomass to produce biofuels and value-added chemicals, which might promote the transition to a more environmentally friendly economy. The expression of these extracellular enzyme genes is tightly controlled at the transcriptional level, which limits their yields. Hitherto our understanding of the regulation of expression of plant biomass-degrading enzyme genes in filamentous fungi has been rather limited. In the present study, regulatory roles of a key regulator, PoxNsdD, were further explored in the soil fungus , contributing to the understanding of gene regulation in filamentous fungi and revealing the biotechnological potential of via genetic engineering.

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“…Other transcription factors involved in biomass utilization have been characterized: ACE1 [ 20 ], ACE2 [ 21 ], ACE3 [ 22 ], BGLR [ 15 ], HAP 2/3/5 complex [ 23 ], PAC1 [ 24 ], PMH20, PMH25, PMH29 [ 22 ], XPP1 [ 25 ], RCE1 [ 26 ], VE1 [ 27 ], MAT1-2-1 [ 28 ], VIB1 [ 29 , 30 ], RXE1/BRLA [ 31 ] and ARA1 [ 32 ]. Moreover, transcription factors involved in the regulation of cellulolytic enzymes have also been characterized in other filamentous fungi: CLR-1 and CLR-2 in Neurospora crassa [ 33 ] or AZF1 [ 34 ], PoxHMBB [ 35 ], PRO1, PoFLBC [ 36 ] and NSDD in Penicillium oxalium [ 37 , 38 ]. Yet, their respective function has not yet been established in T. reesei .…”

Section: Introductionmentioning

confidence: 99%

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30

et al. 2020

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Background The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process. Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei. Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available. A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30. Results Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition. The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile. A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth. Conclusions This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode. Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production. These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.

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Penicillium oxalicum PoFlbC regulates fungal asexual development and is important for cellulase gene expression

Cited by 27 publications

References 60 publications

Gene Regulatory Networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 Inferred by a Computational Biology Approach

Gene Regulatory Networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 Inferred by a Computational Biology Approach

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30

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