Penile alterations at early stage of type 1 diabetes in rats

Tao, Mingfang; Taşdemir, Cemal; Tasdemir, Seda; Shahabi, Ali; Liu, Guiming

doi:10.1590/s1677-5538.ibju.2016.0454

Cited by 11 publications

(7 citation statements)

References 29 publications

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“…The increase of collagen fibers in the tunica albuginea of the penis in T1DM and T2DM mice found in this study was also similar to previous studies [48,49]. Consistent with a previous report [50], the penile cross-sectional area (%) was decreased in DM mice, resulting in atrophy of the penis. The increase of collagen fibers in the testis of animals with DM 72 Day A may have been caused by increased expression of transforming growth factor beta 1 and nitric oxide synthase [51][52][53][54].…”

Section: Discussionsupporting

confidence: 93%

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

Sampannang

Arun

Burawat

et al. 2020

Clin Exp Reprod Med

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Objective: The differences between type 1 and type 2 diabetes mellitus (T1DM and T2DM) in terms of their adverse effects on male reproductive parameters have never been elucidated. This study aimed to distinguish between the effects of the DM types in mice treated with multiple low doses of streptozotocin (STZ) to mimic human T1DM and coadministered a high-fat diet (HFD) to mimic human T2DM.Methods: The T1DM mice were intraperitoneally injected with STZ (40 mg/kg body weight) for 5 days. The T2DM mice received an HFD for 14 days prior to STZ injection (85 mg/kg body weight), followed by continuous feeding of an HFD. Male reproductive parameters were evaluated.Results: The reproductive organs of the DM mice weighed significantly less than those of controls, and the seminal vesicles plus prostates of the T1DM mice weighed less than those of the T2DM mice. Increased sperm abnormalities and incomplete DNA packaging were observed in the DM groups. Sperm concentration and the proportion of normal sperm were significantly lower in the T1DM group. The seminiferous histopathology of DM mice was classified into seven types. The penises of the DM mice were smaller than those of the controls; however, tunica albuginea thickness and the amount of penile collagen fibers were increased in these mice. Round germ cells were abundant in the epididymal lumens of the mice with DM.Conclusion: T1DM adversely affected reproductive parameters to a greater extent than T2DM.

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Section: Discussionsupporting

confidence: 93%

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

Sampannang

Arun

Burawat

et al. 2020

Clin Exp Reprod Med

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“…These data are in agreement with several previous studies, which reveal that both in aged men and aged animals, a decline in erectile capability is associated with a diminishing number of smooth muscle tissue cells [45][46][47][48]. Similar data were also observed in an ED model induced by diabetes [49]. Reduction of erectile function with aging has been extensively reported and related to multiple functional, morphometric, molecular, and cellular changes that lead to significant loss of erectile capability.…”

Section: Plos Onesupporting

confidence: 92%

D-(+)-Galactose-induced aging: A novel experimental model of erectile dysfunction

et al. 2021

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Erectile dysfunction (ED) is defined as the inability to achieve and/or maintain penile erection sufficient for satisfactory sexual relations, and aging is one of the main risk factors involved. The D-(+)-Galactose aging model is a consolidated methodology for studies of cardiovascular aging; however, its potential for use with ED remain unexplored. The present study proposed to characterize a new experimental model for ED, using the D-(+)-Galactose aging model. For the experiments, the animals were randomly divided into three groups receiving: vehicle (CTL), D-galactose 150 mg/kg (DGAL), and D-(+)-galactose 150 mg/Kg + sildenafil 1.5 mg/Kg (DGAL+SD1.5) being administered daily for a period of eight weeks. All of the experimental protocols were previously approved by the Ethics Committee on the Use of Animals at the Federal University of Paraíba n° 9706070319. During the treatment, we analyzed physical, molecular, and physiological aspects related to the aging process and implicated in the development of ED. Our findings demonstrate for the first time that D-(+)-Galactose-induced aging represents a suitable experimental model for ED assessment. This was evidenced by an observed hyper-contractility in corpora cavernosa, significant endothelial dysfunction, increased ROS levels, an increase in cavernous tissue senescence, and the loss of essential penile erectile components.

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“…Therefore, the integrity of intracavernous structures like smooth muscles, endothelium, and nerve terminals are crucially required to provide a normal erection ( Albersen et al, 2011a ). The interest in CS therapy strategies for erectile restoration is growing now to address the multifactorial pathogenesis of ED ( Tao et al, 2017 ). Stem cells (SCs) therapy represents a promising new hope in this field due to their ability to act either by differentiation and direct integration within the recipient tissue or by their paracrine effect through the secretion of various growth factors and cytokines ( Matz et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Urine-Derived Stem Cells Versus Their Lysate in Ameliorating Erectile Dysfunction in a Rat Model of Type 2 Diabetes

et al. 2022

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Background: Diabetic erectile dysfunction (DED) is a significant consequence of diabetes mellitus, and it is a multifactorial phenomenon that has no definitive treatment until now. Many therapeutic options provide symptomatic improvement rather than addressing the underlying etiology or restoring normal function. Stem cell (SC) therapy represents a potential hope in DED management. It is well established that the regenerative effect of stem cells can be attained by their paracrine action and their ability to differentiate into many cell lineages, including endothelial and smooth muscle cells. Hence, we tried to compare the effects of transplantation of urine-derived stem cells (USCs) or their lysate (USC-L) into the corpora cavernosa (CCs) of rats with DED.Materials and Methods: A total of 55 adult male Wistar rats were included in this study. USCs were obtained from ten healthy rats. Another ten rats did not subject to any intervention and served as a control (group I). Type 2 DM and DED were induced in the remaining 35 rats, but DED was tested and proved in only 24 rats, which were randomly divided into three groups (n = 8 in each). The DED group (group II) and either USCs (2 × 106 cells) or their lysate (200 μl) were transplanted into the CCs of each rat in the other two groups (groups III and IV), respectively.Results: Although the DED rats exhibited deterioration in all copulatory functions as compared to the control group, our histopathological, immunohistochemical, and morphometric results revealed that both USCs and USC-L have significantly restored the cavernous spaces, the ultrastructures of the endothelium that line the cavernous spaces, collagen/smooth muscle ratio, and the mean area percentage of α-SMA in the CCs as compared to DED rats. A respectable number of USCs was detected in the CCs of group III at the 4th week after transplantation, but this number significantly declined by the 8th week.Conclusion: Both USCs and USC-L can repair the structure and ultrastructure of CCs and improve the copulatory functions in the DED rat model. However, USC-L could be better used in DED to guard against the strange behavior of USCs after transplantation and their decreased survivability with time.

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Penile alterations at early stage of type 1 diabetes in rats

Cited by 11 publications

References 29 publications

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

D-(+)-Galactose-induced aging: A novel experimental model of erectile dysfunction

Urine-Derived Stem Cells Versus Their Lysate in Ameliorating Erectile Dysfunction in a Rat Model of Type 2 Diabetes

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