Peningkatan Sistem Imun Oleh Kombinasi Ekstrak Etanol Daun Awar – Awar (Ficus Septica Burm. F) Dan Ekstrak Etanol Daun Kelor (Moringa Oleifera) Sebagai Ko-Kemoterapi Kanker Pada Tikus Putih Betina Galur Sprague Dawley Yang Diinduksi Doksorubisin

Abstract

ABSTRAKPenggunaan obat doksorubisin untuk terapi kanker menyebabkan sistem imun tubuh menurun. Daun awar-awar dan daun kelor merupakan tanaman herbal yang memiliki manfaat sebagai agen imunostimulan atau meningkatkan sistem kekebalan tubuh. Adapun tujuan penelitian ini untuk mengetahui efek kombinasi ekstrak etanol daun awar-awar dan ekstrak etanol daun kelor sebagai kokemoterapi kanker pada tikus putih betina galur Sprague Dawley yang terinduksi doksorubisin. Delapan belas ekor tikus Sprague Dawley dibagi menjadi 6 kelompok perlakuan, yaitu kelompok normal diberi NaCl 0,9% 10ml/kgBB, kelompok kontrol positif diberi doksorubisin dosis 4,67 mg/kgBB, kelompok perlakuan dosis tunggal yang masing-masing diberi ekstrak etanol daun awarawar dosis 750 mg/kgBB dan ekstrak etanol daun kelor 500 mg/kgBB, kelompok perlakuan yang masing-masing diberi kombinasi ekstrak etanol daun awar-awar dan daun kelor dengan dosis 1,25 g/kgBB dan dosis 1,75 g/kgBB. Perlakuan dilakukan selama 7 hari berturut turut. Pada hari ke-1 dan ke-4 masing masing kelompok perlakuan kecuali kelompok normal diinduksi doksorubisin 4,67 mg/kgBB secara intramuskuler. Pengambilan darah untuk pemeriksaan limfosit CD3+ dan limfosit CD8+ dilakukan pada hari ke-0 dan ke-7. Hasil penelitian menunjukkan bahwa kombinasi ekstrak etanol daun awar-awar dan daun kelor dosis 1,25 g/kgBB dan dosis 1,75 g/kgBB dapat meningkatkan sistem imun relatif kecil yang secara statistik tidak berbeda signifikan (p>0,05). Namun pemberian dosis tunggal ekstrak etanol daun kelor dosis 500 mg/kgBB memiliki aktivitas sebagai imunostimulator terhadap CD3 + dan CD8 + yang lebih efektif jika dibandingkan dengan pemberian dosis tunggal ekstrak etanol daun awar-awar dosis 750 mg/kgBB. Kata Kunci : doksorubisin, daun awar-awar, daun kelor, limfosit CD3+ dan limfosit CD8+ limfosit.

Effect of glutamine, glucose unhidrate and Moringa oleifera on blood lymphocytes in white mice (Rattus Novergicus) Wistar strain, following induction of a protein energy-deficient diet

“…showed that a single dose of 500 mg/kgBB dose of moringa leaf ethanol extract had more effective activity as an immunostimulator against CD3+ and CD8+ when compared to a single dose of ethanol extract of 750 mg/kgBB, suggesting that ethanol extract groups and moringa leaves had a more effective ability to boost the immune system. 7 And research conducted by Fathir et al (2014:114-120) showed that giving moringa leaf extract can increase the number of CD4+ T cells and CD8+ T cells in all mice infected with salmonella thypi and the administration of high doses of moringa leaf extract causes immunosuppression and can also serve as immunostimulated and immunosuppression in CD4+ T cells and CD8+ mice, this is due to an increase in the number of CD8+ T cells affected by the presence of active substances in moringa leaf extracts. Saponins and flavonoids that can stimulate increased IL-2 in CD4+ T cells and increased IL-2 also allow for an increase in the number of CD8+ T cells.…”

“…According to Sari et al , it was stated that mice given ethanol extract and moringa leaves of 500 mg/kgBB within 7 consecutive days had effective immunostimulatory activity against CD3+ and CD8+. 7 Therefore, the proportion of moringa leaves given according to the weight of mice is 500 mg/1000 gr = 1:2, 80 mg/160 gr = 1: 2. 80 mg = 0.08 gr.…”

“…showed that a single dose of 500 mg/kgBB dose of moringa leaf ethanol extract had an activity as an immunostimulator against CD3+ and CD8+ which was more effective when compared to a single dose of 750 mg/kgBB dose of ethanol extract. 7 Research conducted by Rizqiyah, the administration of moringa flour can increase the number of lymphocytes of wistar rats that experience a condition of significantly less protein energy, said so because moringa has a high protein content and complete amino acid composition. 8 This study was conducted by examining the administration of Glumorry in white mouse lymphocytes (Rattus novergicus) Wistar strain that has been induced with the PEM diet.…”

The Effect of Avicennia marina Leaf Extract Addition To the Artificial Feed On the Growth of Litopenaeus vannamei

“…Mangrove leaves play an important role in improving the survival rate due to their ability to improve shrimp health. Avicennia marina leaf extract contains flavonoid compounds that can act as antimicrobial, antibacterial, antiviral, and immunostimulants (Sari et al, 2015). The mechanism of flavonoids as antibacterial can be divided into three: the inhibition of nucleic acid synthesis, the inhibition of cell membrane function, and the inhibition of energy metabolism (Rijayanti, 2014).Alkaloids are antibacterial, but they work by destruct the peptidoglycan component in bacterial cells, resulting in the formation of imperfect cell wall layers, thereby causing the bacterial cell to die (Mufti et al, 2022).…”

“…Avicennia marina can promote growth, boost the immune system and stimulate the feeding response in aquaculture. Flavonoids can act as antimicrobial, antibacterial, antiviral and immunostimulants (Sari et al, 2015). Adding immunostimulants into the feed has been an effective method to improve non-specific defense systems (Irkin and Yigit, 2015).…”