Penitentiary population of Mycobacterium tuberculosis in Kyrgyzstan: Exceptionally high prevalence of the Beijing genotype and its Russia-specific subtype

Mokrousov, Igor; Valcheva, Violeta; Sovhozova, Nurmira; Aldashev, Almaz; Rastogi, Nalin; Isakova, Jainagul

doi:10.1016/j.meegid.2009.07.007

Cited by 51 publications

(41 citation statements)

References 26 publications

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“…However, available published data show that genotypes similar to those prevalent in Estonia are widely spread across many countries of the former Soviet Union, including the Baltic States, Russia, and Central Asian countries (16,20). Since all these areas have extremely high rates of drug resistance, we believe that our study results are important for the implementation of the assay in the given settings.…”

Section: Discussionmentioning

confidence: 79%

Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

Ignatyeva

Kontsevaya²,

Kovalyov³

et al. 2012

J Clin Microbiol

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eThe rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the performance of the Genotype MTBDRsl assay compared to that of phenotypic drug susceptibility testing (Becton Dickinson Bactec MGIT 960 system) with a test panel of 200 Mycobacterium tuberculosis isolates at four sites in Eastern Europe. The interpretability of the Genotype MTBDRsl assay was over 95%. The sensitivity for the detection of resistance to fluoroquinolones, ethambutol, amikacin, and capreomycin varied between 77.3% and 92.3%; however, it was much lower for kanamycin (42.7%). The sensitivity for the detection of XDR TB was 22.6%. The test specificity was over 82% for all drugs. The assay presents a good screening tool for the rapid detection of resistance to individual second-line drugs and can be recommended for use in countries with a high burden of MDR/XDR TB. The sensitivity for the detection of kanamycin resistance needs improvement.

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Section: Discussionmentioning

confidence: 79%

Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

Ignatyeva

Kontsevaya²,

Kovalyov³

et al. 2012

J Clin Microbiol

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“…Recently, 12% of mixed M. tuberculosis infections were identified by whole-genome sequencing (28); however, this method is limited by the cost, complexity of data analysis, and the short sequencing reads used to account for repeat regions (5). Mixed Beijing and non-Beijing strains caused the most pathogen-pathogen compatibility (10,14,29,30); however, the mixed non-Beijing strains were also reported to be primary in some regions (12,31). Besides, although Beijing strains were commonly present in mixed infections, their spoligotypes usually were masked by the spoligotypes of non-Beijing strains (14).…”

Section: Discussionmentioning

confidence: 99%

Mixed Infections and Rifampin Heteroresistance among Mycobacterium tuberculosis Clinical Isolates

Zheng

Luo

et al. 2015

J Clin Microbiol

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Mixed infections and heteroresistance of Mycobacterium tuberculosis contribute to the difficulty of diagnosis, treatment, and control of tuberculosis. However, there is still no proper solution for these issues. This study aimed to investigate the potential relationship between mixed infections and heteroresistance and to determine the high-risk groups related to these factors. A total of 499 resistant and susceptible isolates were subjected to spoligotyping and 24-locus variable-number tandem repeat methods to analyze their genotypic lineages and the occurrence of mixed infections. Two hundred ninety-two randomly selected isolates were sequenced on their rpoB gene to examine mutations and heteroresistance. The results showed that 12 patients had mixed infections, and the corresponding isolates belonged to Manu2 (n ‫؍‬ 8), Beijing (n ‫؍‬ 2), T (n ‫؍‬ 1), and unknown (n ‫؍‬ 1) lineages. Manu2 was found to be significantly associated with mixed infections (odds ratio, 47.72; confidence interval, 9.68 to 235.23; P < 0.01). Four isolates (1.37%) were confirmed to be heteroresistant, which was caused by mixed infections in three (75%) isolates; these belonged to Manu2. Additionally, 3.8% of the rifampin-resistant isolates showing no mutation in the rpoB gene were significantly associated with mixed infections ( 2 , 56.78; P < 0.01). This study revealed for the first time that Manu2 was the predominant group in the cases of mixed infections, and this might be the main reason for heteroresistance and a possible mechanism for isolates without any mutation in the rpoB gene to become rifampin resistant. Further studies should focus on this lineage to clarify its relevance to mixed infections.

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“…However, a recent study indicated that these loci were not extraordinarily unstable compared to other loci and IS6110 RFLP patterns (23). Furthermore, several studies in Japan and countries of the former Soviet Union indicated the necessity to include these loci to increase the discriminatory power and differentiate epidemically unrelated Beijing strains which appeared to be clustered by standard 15-or 24-locus VNTR typing (23,35,36). In our study, the relatively conserved profiles of hypervariable alleles within VNTR-15-and SNP-based clusters may also prove their clonal stabilities during transmissions.…”

Section: Discussionmentioning

confidence: 99%

Combination of Single Nucleotide Polymorphism and Variable-Number Tandem Repeats for Genotyping a Homogenous Population of Mycobacterium tuberculosis Beijing Strains in China

et al. 2012

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Penitentiary population of Mycobacterium tuberculosis in Kyrgyzstan: Exceptionally high prevalence of the Beijing genotype and its Russia-specific subtype

Cited by 51 publications

References 26 publications

Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

Mixed Infections and Rifampin Heteroresistance among Mycobacterium tuberculosis Clinical Isolates

Combination of Single Nucleotide Polymorphism and Variable-Number Tandem Repeats for Genotyping a Homogenous Population of Mycobacterium tuberculosis Beijing Strains in China

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