Pennisetum glaucum Na+/H+ antiporter confers high level of salinity tolerance in transgenic Brassica juncea

Rajagopal, Divya; Agarwal, Pradeep K.; Tyagi, Wricha; Singla‐Pareek, Sneh L.; Reddy, M. K.; Sopory, Sudhir K.

doi:10.1007/s11032-006-9052-z

Cited by 88 publications

(29 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, NHX1 from Pennisetum glaucum has been reported to confer high level of salinity tolerance when overexpressed in B. juncea. Transgenic B. juncea plants over expressing PgNHX1 withstand 300mM salt stress till the seed setting stage and exhibited normal growth phenotype without much loss of seed yield (Rajagopal et al, 2007). Taken together these reports establish an important role played by the single gene i.e., NHX in controlling ion homeostasis under salinity stress in plants.…”

Section: Engineering Ion Transpotersmentioning

confidence: 63%

Towards salinity tolerance in Brassica: an overview

Purty

Kumar

Singla‐Pareek

et al. 2008

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

Among the various abiotic stresses limiting the crop productivity, salinity stress is a major problem, which needs to be addressed and answered urgently. Since members of Brassicaceae are important contributor to total oilseed production, there is an immediate need being felt to raise Brassica plants which would be more suitable for saline and dry lands in years to come. One of the suggested way to develop salinity tolerant Brassica plants is to make use of the broad gene pool available within the family. Efforts of breeders have been successful in such endeavors to a large extent and several salinity tolerant Brassica genotypes have been developed within India and elsewhere. On the other hand, transgenic technology will undoubtedly continue to aid the search for the cellular mechanisms that confer tolerance, but the complexity of the trait is likely to mean that the road to engineer such tolerance into sensitive species will not be easy. However, with increasing number of reports available for suitable genetic transformation for various Brassica genotypes, there is a hope that salinity tolerance can be improved in this important crop plant. In this direction, the complete genome sequence of related wild plants such as Arabidopsis or crop plants such as rice can also serve as a platform for identification of "candidate genes". Recently, complete genome sequencing of the Brassica genomes has also been initiated with the view that availability of such useful information can pave way towards raising Brassica with improved tolerance towards these stresses. In the present paper, we discuss the success obtained so far; in raising brassica genotypes with improved salinity tolerance employing both plant breeding and/or genetic engineering tools.

show abstract

Section: Engineering Ion Transpotersmentioning

confidence: 63%

Towards salinity tolerance in Brassica: an overview

Purty

Kumar

Singla‐Pareek

et al. 2008

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…antiporter, which catalyzes a large accumulation of Na ? in the vacuole of AtNHX1-expressing plants in highly saline conditions (Apse et al 1999;Lü et al 2005;Rajagopal et al 2007). However, more recent evidence has revealed that plant NHXs might be Na ?…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have shown that overexpression of the AtNHX1 gene confers salt tolerance to Arabidopsis plants and various other plant species (Apse et al 1999;Xue et al 2004;He et al 2005;Leidi et al 2010). Furthermore, overexpession of AtNHX1 orthologs, such as AgNHX1 of Atriplex gmelini C. A. Mey, OsNHX1 of Oryza sativa L., BnNHX1of Brassica napus L., GhNHX1 of Gossypium spp., HbNHX1 of Hordeum uhulgare L., PgHNX1 of Pennisetum glaucum L., ThNHX1 of Thellungiella halophila, AtNHX3, AtNHX4, and MdNHX1 of Malus confer high salt tolerance to transgenic plants (Ohta et al 2002;Fukuda et al 2004;Wang et al 2004;Wu et al 2004;2005;Lü et al 2005;Rajagopal et al 2007;Verma et al 2007;Wang et al 2007;Liu et al 2008;Wu et al 2009;Li et al 2010). Studies on the Class-II antiporters, have been reported only for the Arabidopsis AtNHX5 (Shi et al 2008) and the tomato LeNHX2 (Rodriguez- Rosales et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtNHX5 improves tolerance to both salt and water stress in rice (Oryza sativa L.)

Lin

et al. 2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Overexpression of NHX genes has been previously shown to improve salt tolerance of transgenic plants. In this study, transgenic rice plants overexpressing AtNHX5 showed not only high salt tolerance, but also high drought tolerance. Measurements of ion levels indicated that Na ? and K ? contents were all higher in AtNHX5 overexpressing shoots than in wild type (WT) shoots in high saline conditions. After exposure to water deficiency and salt stress, the WT plants all died, while the AtNHX5 overexpressing rice plants had a higher survival rate, dry weight, leaf water content, and leaf chlorophyll contents, accumulated more proline, and had less membrane damage than the WT plants. In addition, seeds of both transgenic and WT plants germinated on 1/2 MS medium supplemented with 250 mM mannitol, but overexpression of AtNHX5 improved the shoot growth of the seedlings. Taken together, the results indicate that AtNHX5 gene could enhance the tolerance of rice plants to multiple environmental stresses by promoting the accumulation of more effective osmolytes (ions or proline) to counter the osmotic stress caused by abiotic factors.

show abstract

“…For example, in shoots of barley, the Na 1 content was about 10 mg/g DW [44]. When a vacuolar Na 1 /H 1 antiporter was ectopically over-expressed, leaves of transgenic Brassica juncea [45] and Oryza sativa [46] exhibited Na 1 content at about 6 and 10 mg/g DW, respectively.…”

Section: The Bpp Methods Efficiently Removes Salt Ions From S Europaementioning

confidence: 99%

A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

et al. 2007

View full text Add to dashboard Cite

Protein extraction from plants like the halophyte Salicornia europaea has been problematic using standard protocols due to high concentrations of salt ions in their cells. We have developed an improved method for protein extraction from S. europaea, which allowed us to remove interfering compounds and salt ions by including the chemicals borax, polyvinylpolypyrrolidone, and phenol. The comparative study of this method with several other protocols using NaCl-treated S. europaea shoots demonstrated that this method gave the best distinction of proteins on 2-DE gels. This protocol had a wide range of applications as high yields and good distinction of 1-DE gels for proteins isolated from twelve other plants were rendered. In addition, we reported results of 2-DE using the recalcitrant tissue of the S. europaea roots. We also demonstrated that this protocol is compatible with proteomic analysis as eight specific proteins generated by this method have been identified by MS. In conclusion, our newly developed protein extraction protocol is expected to have excellent applications in proteomic studies of halophytes.

show abstract

Pennisetum glaucum Na+/H+ antiporter confers high level of salinity tolerance in transgenic Brassica juncea

Cited by 88 publications

References 56 publications

Towards salinity tolerance in Brassica: an overview

Towards salinity tolerance in Brassica: an overview

Overexpression of AtNHX5 improves tolerance to both salt and water stress in rice (Oryza sativa L.)

A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

Contact Info

Product

Resources

About