Pennogenyl saponins induce cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells

Long, Fangyi; Chen, Ya-Shu; Zhang, Liang; Kuang, Xi; Yu, Yan; Wang, Liang-Fen; Liu, Xiaojiao; Zhou, Yifan; Sang, Na; Du, Jun-Rong

doi:10.1016/j.jep.2014.12.065

Cited by 26 publications

(19 citation statements)

References 23 publications

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“…markedly inhibited cell viability in HepG2, MCF-7 and PC-3 cells. The two compounds also induced apoptosis and caused cell cycle arrest in HepG2 cells affecting multiple targets, including mitochondrial caspasedependent and independent pathway, cyclin-dependent kinase 1 activation or PI3K/Akt signalling (Long et al 2015). In another study P. polyphylla steroidal saponins decreased the viability of human lung cancer A549 cells through both apoptosis and autophagy, with the activation of caspase-8 and 3 and PARP cleavage for the former, and up-regulation of Beclin1 and conversion from LC3 I to LC3 II for the latter process, respectively (He et al 2014).…”

Section: Results Of In Vitro Studiesmentioning

confidence: 99%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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Steroidal saponins are a group of glycosides widely distributed among monocotyledonous families. They exert a wide spectrum of biological effects including cytotoxic and antitumor properties which are the most studied. This review is an update of our previous paper-Saponins as cytotoxic agents (Podolak et al. in Phytochem Rev 9:425-474, 2010) and covers studies that were since published (2010-2018). In this paper we refer to steroidal saponins presenting results of cytotoxicity studies, mechanisms of action and structure-activity relationships.

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Section: Results Of In Vitro Studiesmentioning

confidence: 99%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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“…Besides, in vitro experiments with HepG2 cells have also shown this inhibition effect of Rhizoma Paridis total saponins (Cheng et al, 2008). For the individual monomeric saponin from Rhizoma Paridis, PPI and PP7 were reported to inhibit the growth of hepatoma xenografts in nude mice (Chen et al, 2014) and the proliferation of HepG2 cells (Long et al, 2015). However, the effect of PP2 on liver cancers is still unexplored and not well defined.…”

Section: Discussionmentioning

confidence: 99%

Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-κB pathway

Pang

Yang

et al. 2020

Biology Open

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The morbidity and mortality of primary liver cancer is one of the highest amongst all cancers. Deficiency of effective treatment and characteristics of cancer metastasis are believed to be responsible for this situation, thus a great demand is required for new agent development. Polyphyllin II (PP2), an important steroidal saponin extracted from Rhizoma Paris, has emerged as a potential anticancer agent, but the effects of PP2 in liver cancers and its underlying mechanisms remain unexplored. In our study, we found that PP2 could remarkably suppress the proliferation of two liver cancer cell lines, HepG2 and BEL7402, resulting in significant cell death. Besides, low doses of PP2 have displayed properties that inhibit cellular motility and invasion of liver cancer cells. In addition, we have found that PP2-mediated cofilin activity suppression was implicated in the inhibition of liver cancer cell motility. Decreased expression of two major hydrolytic enzymes (MMP2/MMP9), through the AKT/NF-κB signaling pathway may also be also responsible for this process. Rescue experiments done with either non-phosphorylatable mutant cofilin-1 (S3A) transfection or an activator of the AKT pathway significantly reversed the inhibition effects of PP2 on liver cancer cells. Taken together, we report a potential agent for liver cancer treatment and reveal its underlying mechanisms.

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“…For example, Long et al described anticancer effects with Pennogenyl saponins, the characterized components of Rhizoma Paridis. These effects were associated with the induction of apoptosis and blockage of cell cycle progression through multiple targets in human HCC HepG2 cells (Long et al, 2015). Others reported similar benefits with Glycosmis pentaphylla (Retz.)…”

Section: Discussionmentioning

confidence: 87%