Penta-acetyl geniposide-induced C6 glioma cell apoptosis was associated with the activation of protein kinase C-delta

Peng, Chiung Huei; Tseng, Tsui Hwa; Liu, Jer Yuh; Hsieh, Yi‐Hsien; Huang, Cheng‐Yi; Hsu, Shu Ping; Wang, Chau Jong

doi:10.1016/j.cbi.2004.01.003

Cited by 27 publications

(22 citation statements)

References 37 publications

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“…5). This result is consistent with previous studies that NH 2 and COOH termini of Bax are rich in glycine and hydroxyl amino acids such as serine and threonine (48,49), which are potential targets of p38MAPK, and are critical for regulating the subcellular distribution of Bax (27). In human promonocytic cells, the rapid phosphorylation of p38MAPK suggests that this kinase plays an early role in the regulation of the cadmium-provoked apoptosis (50).…”

Section: Discussionsupporting

confidence: 93%

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P38 MAP Kinase Mediates Apoptosis After Genipin Treatment in Non^|^ndash;Small-Cell Lung Cancer H1299 Cells via a Mitochondrial Apoptotic Cascade

et al. 2013

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Abstract. Genipin, an active constituent of Gardenia fruit, has been reported to show an antitumor effect in several cancer cell systems. Here, we demonstrate how genipin exhibits a strong apoptotic cell death effect in human non-small-cell lung cancer H1299 cells. Genipin-mediated decrease in cell viability was observed through apoptosis as demonstrated by induction of a sub-G 1 peak through flow cytometry, DNA fragmentation measured by TUNEL assay, and cleavage of poly ADP-ribose-polymerase. During genipin-induced apoptosis, the mitochondrial execution pathway was activated by caspase-9 and -3 activation as examined by a kinetic study, cytochrome c release, and a dose-dependent increase in Bax/Bcl-2 ratio. A search for the downstream pathway reveals that genipin-induced apoptosis was mediated by an increase in phosphorylated p38MAPK expression, which further activated downstream signaling by phosphorylating ATF-2. SB203580, a p38MAPK inhibitor, markedly blocked the formation of TUNEL-positive apoptotic cells in genipin-treated cells. Besides, the interference of p38MAPK inhibited Bax expression and cytochrome c release. Altogether, our observations imply that genipin causes increased levels of Bax in response to p38MAPK signaling, which results in the initiation of mitochondrial death cascade, and therefore it holds promise as a potential chemotherapeutic agent for the treatment of H1299 cells.

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Section: Discussionsupporting

confidence: 93%

“…It has long been used in traditional medicine formulations for treatment of inflammation and hepatic disorders (1,2). Genipin reacts spontaneously with amino acids to form blue pigments that are widely used in the food industry, and it is also an effective crosslinking reagent for biological tissue fixation (3).…”

Section: Introductionmentioning

confidence: 99%

P38 MAP Kinase Mediates Apoptosis After Genipin Treatment in Non^|^ndash;Small-Cell Lung Cancer H1299 Cells via a Mitochondrial Apoptotic Cascade

et al. 2013

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“…Phosphorylation of PKC at 64 and 287 is also essential for the apoptotic effect observed after etoposide treatment [233][234][235]. Furthermore, pentaacetyl geniposide-induced apoptosis requires the interaction of activated neutral sphingomyelinase and p75, which is mediated by activated PKC [236,237]. PKC -mediated ROS production has also been indicated to increase paraquat (1,1 -dimethyl-4,4 -bipyridinium)-induced glioma cell death [238].…”

Section: Gastrointestinal Stromal Tumormentioning

confidence: 99%

Protein Kinase C (PKC) Isozymes and Cancer

Kang

2014

New Journal of Science

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Protein kinase C (PKC) is a family of phospholipid-dependent serine/threonine kinases, which can be further classified into three PKC isozymes subfamilies: conventional or classic, novel or nonclassic, and atypical. PKC isozymes are known to be involved in cell proliferation, survival, invasion, migration, apoptosis, angiogenesis, and drug resistance. Because of their key roles in cell signaling, PKC isozymes also have the potential to be promising therapeutic targets for several diseases, such as cardiovascular diseases, immune and inflammatory diseases, neurological diseases, metabolic disorders, and multiple types of cancer. This review primarily focuses on the activation, mechanism, and function of PKC isozymes during cancer development and progression.

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“…Genipin, an aglycone derived from an iridoid glycoside called geniposide present in fruit of Gardenia jasminoides Ellis, has long been used as a traditional oriental medicine for the treatment of hepatic disorders and inflammatory diseases (1). Genipin exerts anti-inflammatory, anti-angiogenic, antiproliferative and hepatoprotective activities in several cell lines including murine macrophage cells, human umbilical vein endothelial cells, human prostate cancer cells, and human hepatocarcinoma cells (2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Genipin, a constituent of Gardenia jasminoides Ellis, induces apoptosis and inhibits invasion in MDA-MB-231 breast cancer cells

Kim

Jeong²,

Moon

2011

Oncol Rep

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Abstract. Genipin, a constituent of Gardenia jasminoides Ellis, is used in the treatment of hepatic disorders and inflammatory diseases in traditional medicine. Although mounting evidence suggests an anti-tumor activity of genipin in several cancer cell systems, the inhibitory effect of genipin on the growth of breast cancer cells has not been reported yet. The present study aimed to investigate the anti-proliferative activity of genipin in MDA-MB-231 human breast cancer cells. Herein, we showed that genipin efficiently induced apoptosis in MDA-MB-231 cells by the down-regulation of Bcl-2, up-regulation of Bax and proteolytic activation of caspase-3. Activation of JNK and p38 MAPK also increased by genipin. Importantly, genipin significantly inhibited invasive and migratory phenotypes of MDA-MB-231 cells. Taken together, this study demonstrates that genipin induces apoptosis and inhibits invasive/migratory abilities of highly invasive MDA-MB-231 human breast cancer cells, suggesting a potential application of genipin as a chemopreventive agent that may prevent or alleviate metastatic breast cancer.

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Penta-acetyl geniposide-induced C6 glioma cell apoptosis was associated with the activation of protein kinase C-delta

Cited by 27 publications

References 37 publications

P38 MAP Kinase Mediates Apoptosis After Genipin Treatment in Non^|^ndash;Small-Cell Lung Cancer H1299 Cells via a Mitochondrial Apoptotic Cascade

P38 MAP Kinase Mediates Apoptosis After Genipin Treatment in Non^|^ndash;Small-Cell Lung Cancer H1299 Cells via a Mitochondrial Apoptotic Cascade

Protein Kinase C (PKC) Isozymes and Cancer

Genipin, a constituent of Gardenia jasminoides Ellis, induces apoptosis and inhibits invasion in MDA-MB-231 breast cancer cells

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