Pentamycin shows high efficacy against Trichomonas vaginalis

Kranzler, Markus; Syrowatka, Michael; Leitsch, David; Winnips, C.; Walochnik, Julia

doi:10.1016/j.ijantimicag.2014.12.024

Cited by 17 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other promising candidate is pentamycine, a macrolide antibiotic used for fungal and bacterial vaginitis with high activity against T. vaginalis . The effect is prompt and independent of under-lying MTZ resistance 177.…”

Section: Treating Trichomoniasis: Are We Succeeding?mentioning

confidence: 85%

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

Menezes

Frasson

Tasca

2016

MIC

119

View full text Add to dashboard Cite

Etiology: Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. Transmission: Trichomoniasis is transmitted by sexual intercourse and transmission via fomites is rare. Epidemiology, incidence and prevalence: The WHO estimates an incidence of 276 million new cases each year and prevalence of 187 million of infected individuals. However, the infection is not notifiable. Pathology/Symptomatology: The T. vaginalis infection results in a variety of clinical manifestations - in most cases the patients are asymptomatic, but some may develop signs typically associated to the disease. Importantly, the main issue concerning trichomoniasis is its relationship with serious health consequences such as cancer, adverse pregnancy outcomes, infertility, and HIV acquisition. Molecular mechanisms of infection: To achieve success in parasitism trichomonads develop a complex process against the host cells that includes dependent- and independent-contact mechanisms. This multifactorial pathogenesis includes molecules such as soluble factors, secreted proteinases, adhesins, lipophosphoglycan that culminate in cytoadherence and cytotoxicity against the host cells. Treatment and curability: The treatment with metronidazole or tinidazole is recommended; however, cure failures remain problematic due to noncompliance, reinfection and/or lack of treatment of sexual partners, inaccurate diagnosis, or drug resistance. Therefore, new therapeutic alternatives are urgently needed. Protection: Strategies for protection including sexual behavior, condom usage, and therapy have not contributed to the decrease on disease prevalence, pointing to the need for innovative approaches. Vaccine development has been hampered by the lack of long-lasting humoral immunity associated to the absence of good animal models.

show abstract

Section: Treating Trichomoniasis: Are We Succeeding?mentioning

confidence: 85%

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

Menezes

Frasson

Tasca

2016

MIC

119

View full text Add to dashboard Cite

show abstract

“…Fungichromin, a polyene macrolide called pentamycin, was previously isolated from Streptomyces pentaticus as an antifungal antibiotic showing antifungal activity against various fungal pathogens. Fungichromin has already been approved for the topical treatment of bacterial and fungal vaginitis and administered as a vaginal tablet [17]. In this study, we found that Streptomyces sp.…”

Section: Structure Determination Of Antifungal Compoundmentioning

confidence: 53%

Control Efficacy of Streptomyces sp. A501 against Ginseng Damping-off and Its Antifungal Substance

et al. 2017

View full text Add to dashboard Cite

Ginseng damping-off, caused by the fungal pathogens Rhizoctonia solani and Pythium sp., is a critical disease in ginseng seedling. In a continuing effort to find microorganisms with the potential of acting as a biocontrol agent against Rhizoctonia damping-off, we found that a Streptomyces sp. A501 showed significant antifungal activity against Rhizoctonia solani. In field experiment to test the efficacy of Streptomyces sp. A501 in controlling ginseng damping-off, the incidence of damping-off disease was meaningfully reduced when ginseng seeds were soaked in the culture broth of Streptomyces sp. A501 before sowing. To perform characterization of the antifungal compound, we isolated it from the culture broth of strain A501 through Diaion HP-20 and silica gel column chromatographies and preparative high-performance liquid chromatography. The structure of the antifungal compound was assigned as fungichromin by spectroscopic methods, mainly nuclear magnetic resonance and electrospray ionization-mass analysis.

show abstract

“…FC, as a macrolide compound, has been reported to have a wide range of antifungal activities. [10][11][12] Here, its impact on C. albicans biofilm formation is first investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Also, the treatment efficacy of antifungal agents is limited because of toxicity and resistance. 8,9) Fungichromin (FC), as one of polyene antibiotics, has been reported for its in vitro antifungal properties including C. albicans [10][11][12] ; however, until now, the activity of FC against sessile cells in C. albicans biofilm has never been studied. The aim of the present study is to explore the inhibitory effect of FC on the formation of C. albicans biofilm so as to evaluate the potential therapeutic implication for biofilm-associated candidal infections.…”

Section: )mentioning

confidence: 99%

The Activity of Fungichromin against the Formation of <i>Candida albicans</i> Biofilm

Wang

et al. 2016

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The effect of fungichromin (FC) on the formation of Candida albicans biofilm was assessed using 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction method, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Results showed that FC revealed an inhibitory effect on the formation of C. albicans biofilm in a dose-dependent manner with a minimum inhibitory concentration (MIC) of 10 µg/mL. Over 80% of biofilm formation was inhibited by FC at the concentration of 40 µg/mL when compared with the control. Similarly, real-time PCR showed that the expression of the genes such as ALS1, ALS3, HWP1, EFG1, HYR1, CPH1 and BCR1 appeared to be remarkably affected by FC at the concentration of 20 µg/mL during the biofilm formation. In addition, FC could also induce the apoptosis of C. albicans cells in a dose-dependent manner. Therefore, FC displayed potent activity against the formation of C. albicans biofilm in vitro and played an important role in reducing the incidence of device-associated infections. Key words fungichromin (FC); Candida albicans; biofilmCandida albicans is a dimorphic yeast that can be either a commensal or an opportunistic pathogen with the capability to cause a variety of infections, ranging from superficial mucous membrane infection to life-threatening systemic diseases. 1,2)Invasive candidiasis carries unacceptably high mortality, which is reported to be as high as 40-60%.3) One of the major reasons for this phenomenon is that candidiasis can adapt to a variety of environmental conditions through attaching to the surface and growing in microbial community in a mode of biofilm.4) Biofilm can be formed not only on host tissues, but also on prosthesis and indwelling medical devices, such as urinary and vascular catheters, (dental) implants, prostheses, and heart valves.5,6) C. albicans biofilm can lead to a significantly increased resistance to conventional antifungal drugs. 7)Another important reason for the low success rate in treating candidiasis is due to the limited number of antifungal agents. Also, the treatment efficacy of antifungal agents is limited because of toxicity and resistance. 8,9) Fungichromin (FC), as one of polyene antibiotics, has been reported for its in vitro antifungal properties including C. albicans [10][11][12] ; however, until now, the activity of FC against sessile cells in C. albicans biofilm has never been studied. The aim of the present study is to explore the inhibitory effect of FC on the formation of C. albicans biofilm so as to evaluate the potential therapeutic implication for biofilm-associated candidal infections. MATERIALS AND METHODS Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC)C. albicans was prepared using broth dilution technique. FC with the gradient concentration of 0.625-80 µg/mL was prepared by two-fold serial dilution technique through RPMI 1640 medium buffered to pH 7.0 with MOPS buffer, and then inoculated with 100 µL of C. albicans suspension. Fluconazole...

show abstract

Pentamycin shows high efficacy against Trichomonas vaginalis

Cited by 17 publications

References 11 publications

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

Control Efficacy of Streptomyces sp. A501 against Ginseng Damping-off and Its Antifungal Substance

The Activity of Fungichromin against the Formation of <i>Candida albicans</i> Biofilm

Contact Info

Product

Resources

About