Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

Tang, Qiuxia; Dang, Ruyi; Qiu, Li; Yang, Zhicong; Wang, Xinglong

doi:10.1101/394072

“…No such particles have been found for Ad2 or Ad5 (12)(13)(14). This particle shows a remarkable cell penetration ability with 200-300 thousand VLPs per cultured cell (15,16); thus, it can be engineered to deliver several millions of foreign cargo molecules into a single target cell (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this preprint (which was this version posted August 17, 2018. ; https://doi.org/10.1101/394072 doi: bioRxiv preprint causing a mortality of approximately 30% to 70% (27,28). Inactivated vaccines (29)(30)(31), attenuated live vaccines (32,33), and recombinant vaccines have been used to control HHS and these types of vaccines have been proven to be effective in protecting against FAdV infection. In a previous study, scientists purified Pt-Dd produced by FAdV-8b via density gradient centrifugation, which induced neutralizing antibodies and cytotoxic T-cell responses in breeders, as well as successfully prevented clinical disease in progeny via maternal antibody transfer (34).…”

Section: Introductionmentioning

confidence: 99%

Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

Wang

¹

,

Tang

²

,

Qiu

³

et al. 2019

Vaccine

Self Cite

View full text Add to dashboard Cite

In some serotypes of adenovirus (Ad), the penton base protein and attached trimeric fiber assemble into dodecameric virus-like particles called penton-dodecahedron (Pt-Dd), which can internalize into cells and can be used to deliver the vaccine antigen and drugs. Fowl adenovirus serotype 4 (FAdV-4) is an important poultry pathogens and causing seriously economic lost to poultry industry in China and several other counties. The produce of Pt-Dd in FAdV-4 infected cells as well as in those infected with the recombinant human Ad expressing fiber-1, fiber-2, and penton base was determine by Transmission electron microscopy (TEM). For the First time, we proved that FAdV-4 produced Pt-Dd in infected cells, which can also be assembled by the overexpressed recombinant proteins fiber-1, fiber-2, and penton base. Pt-Dd, as well as the recombinant proteins fiber-1, fiber-2, and penton base, were then used to immunize chickens. The humoral immune response, cell mediate immune response (CMI), and challenge results were used to evaluate the immune efficacy of the vaccine candidates. Pt-Dd induced the highest level of enzyme-linked immunosorbent assay antibodies and high levels of CMI, showing a significantly (p < 0.05) high level of interferonγ, interleukin-4, and major histocompatibility complex II expressions in peripheral blood mononuclear cells at 48 h post-infection. The challenge results showed that Pt-Dd, inactivated FAdV-4 vaccine, as well as fiber-1 induced the best protection (100%), followed by fiber-2 (80%) and penton (67%). The present study showed that FAdV-4 -Pt-Dd and recombinant fiber-1 are good FAdV-4 vaccine candidates and could be used to replace the tissue-sourced inactivated FAdV-4 vaccine.

show abstract

“…Adenovirus vectors are the most commonly used viral vectors in gene therapy and the delivery of vaccine antigens. Adenovirus vectors used as vaccines are mostly replication-defective, with certain essential viral genes deleted and replaced by a foreign gene expression cassette [11,12]. Here, the MSTN-SST fusion open reading frame was inserted into the Ad5 genome, which was used to express the protein MSTN-SST.…”

Section: Discussionmentioning

confidence: 99%

Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

Xu

¹

,

Zhang²,

Liu

³

et al. 2020

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

show abstract

“…It was stored at −80 °C after puri cation until assayed. The sera of the mice collected at different times were detected with the ELISA plates as reported [12]. Commercial peroxidase-conjugated rabbit anti-mice IgG (Sigma, Shanghai, China) was used as the secondary antibody.…”

Section: Histopathology Testsmentioning

confidence: 99%

Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

Xu

¹

,

Zhang²,

Liu

³

et al. 2020

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background Myostatin (MSTN), a type of transforming growth factor, can negatively regulate skeletal muscle growth. Immunisation of mice with recombinant yeast expressing MSTN increased bodyweight and muscle composition of them. Somatostatin (SST) is an inhibitory effector of growth hormone (GH), and active immunisation against SST with a DNA vaccine improves the growth performance of piglets via an influence on GH secretion. Here, a recombinant adenovirus was constructed and used to deliver MSTN-SST to mice to achieve the goal of regulation the growth performance of the mice. Results A recombinant adenovirus, rAd-MSTN-SST, expressing the MSTN-SST fusing protein was successfully rescued in HEK293A cells. The expressions of MSTN-SST were confirmed by western blotting and indirect immunofluorescence. Mice immunised with rAd-MSTN-SST were successfully induced to have an immune response against MSTN-SST, which increased their growth rate and muscle mass. In addition, a booster immunisation was beneficial in terms of higher antibody response and mouse growth rate. Conclusions Fusing protein MSTN-SST is a good candidate for regulating animal growth rate and muscle mass. Adenovirus can be used as a vector for delivering MSTN and SST to animals.

show abstract

Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

Cited by 3 publications

References 43 publications

Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

Adenovirus Expressing the Myostatin-Somatostatin-fusing Gene Increases Mouse Growth Rate

Contact Info

Product

Resources

About