Pentose Phosphate Pathway of Erythrocytes in Uremia

Markkanen, T; Peltola, O.; Forsström, J; Himanen, P

doi:10.1159/000208469

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…We found that miR-146a-5p stimulated the expression of G6PD and TKT, the genes encoding two key enzymes of the oxidative and non-oxidative PPP branches, respectively. Interestingly, it was shown that transketolase (TKT) activity correlates with creatinine levels in uremic patients [18], which may possibly partially explain the observation that creatinine levels increased following miR-146a-5p transfection. Remarkably, other reports also demonstrated that creatinine concentrations can be affected by miRNAs [19,20].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-Mediated Metabolic Reprograming in Renal Cancer

Bogusławska

Popławski

Alseekh

et al. 2019

Cancers

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Metabolic reprogramming is one of the hallmarks of renal cell cancer (RCC). We hypothesized that altered metabolism of RCC cells results from dysregulation of microRNAs targeting metabolically relevant genes. Combined large-scale transcriptomic and metabolic analysis of RCC patients tissue samples revealed a group of microRNAs that contribute to metabolic reprogramming in RCC. miRNAs expressions correlated with their predicted target genes and with gas chromatography-mass spectrometry (GC-MS) metabolome profiles of RCC tumors. Assays performed in RCC-derived cell lines showed that miR-146a-5p and miR-155-5p targeted genes of PPP (the pentose phosphate pathway) (G6PD and TKT), the TCA (tricarboxylic acid cycle) cycle (SUCLG2), and arginine metabolism (GATM), respectively. miR-106b-5p and miR-122-5p regulated the NFAT5 osmoregulatory transcription factor. Altered expressions of G6PD, TKT, SUCLG2, GATM, miR-106b-5p, miR-155-5p, and miR-342-3p correlated with poor survival of RCC patients. miR-106b-5p, miR-146a-5p, and miR-342-3p stimulated proliferation of RCC cells. The analysis involving >6000 patients revealed that miR-34a-5p, miR-106b-5p, miR-146a-5p, and miR-155-5p are PanCancer metabomiRs possibly involved in global regulation of cancer metabolism. In conclusion, we found that microRNAs upregulated in renal cancer contribute to disturbed expression of key genes involved in the regulation of RCC metabolome. miR-146a-5p and miR-155-5p emerge as a key “metabomiRs” that target genes of crucial metabolic pathways (PPP (the pentose phosphate pathway), TCA cycle, and arginine metabolism).

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Section: Discussionmentioning

confidence: 99%

MicroRNA-Mediated Metabolic Reprograming in Renal Cancer

Bogusławska

Popławski

Alseekh

et al. 2019

Cancers

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“…The red cell TKA increases in pernicious anaemia (4), and sometimes in uraemia (12), whereas it decreases in thiamine deficiency states (3), in post gastrectomy states (6), chronic alcoholism (8), dementia senilis (8) and hepatic porphyrias (11).…”

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confidence: 99%

Pentose Phosphate Pathway Inhibition in Blood Cells during Anticonceptive Hormone Therapy

Markkanen¹,

Himanen²,

Peltola³

1974

Pharmacology

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The transketolase activity (TKA) of the white and red blood cells of anticonceptive pill (ACP) users was studied in an unselected sample population. Compared with healthy controls of corresponding age, the white cell TKA in the ACP groups was lowered in about 30 %, and the red cell TKA in about 15 % of the subjects studied. The most pronounced TKA inactivation was observed in the group taking pills that contained mestranol and megestrol acetate. The glucose tolerance tests were normal. Discontinuing the hormones normalized the white cell TKA.

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