Following a brief discussion of the literature and intellectual history of Rome’s river port, this article presents preliminary results from a mechanized coring survey of the Forum Boarium valley. Conducted in 2015, this survey produced empirical evidence on prehistoric human activity Rome’s floodplain and acquired substantial data on the paleolandscape of the region, including the shifting position of the Tiber River and the discovery of lacustrine deposits in the valley. Additionally, consideration is given to the advantages and limitations posed by the natural landscape during the origins of settlement and early urban development at Rome.