Pepper CabZIP63 acts as a positive regulator during<i>Ralstonia solanacearum</i>or high temperature–high humidity challenge in a positive feedback loop with CaWRKY40

Shen, Lei; Liu, Zhiqin; Yang, Sheng; Yang, Tong; Liang, Jiaqi; Wen, Jiayu; Liu, Yanyan; Li, Jiazhi; Shi, Lanping; Tang, Qian; Shi, Wei; Hu, Jiong; Liu, Cailing; Zhang, Yangwen; Lin, Wei; Wang, Rongzhang; Yu, Huanxin; Mou, Shaoliang; Hussain, Ammar; Wei, Cheng; Cai, Hanyang; He, Li; Guan, Deyi; Wu, Yang; He, Shuilin

doi:10.1093/jxb/erw069

Cited by 74 publications

(77 citation statements)

References 89 publications

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“…This indicated that CaBPM4 silencing aggravated P. capsici infection in pepper by diminishing the defense system of pepper plants. A similar result was also observed in CabZIP63-silenced plants, where the transcript levels of the defense-related genes CaPR1 and CaDEF1 were significantly lower than those in the control pepper plants under R. solanacearum infection [54]. Similarly, CaPTI1 and CanTF silencing also altered the expression levels of CaPR1 [55], CaDEF1 [56], and CaSAR82 [57] as well as the root activity of silenced plants relative to those in control plants [38,45].…”

Section: Discussionsupporting

confidence: 68%

Contribution of CaBPM4, a BTB Domain–Containing Gene, to the Response of Pepper to Phytophthora capsici Infection and Abiotic Stresses

Liu

Zhang

et al. 2019

Agronomy

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The Broad-complex Tramtrack and Bric-a-brac (BTB) domain participates in plant responses to biotic and abiotic stresses, however its role is unknown in pepper plants. CaBPM4 has meprin and TRAF homology (MATH) and BTB domains at its N-and C-termini, respectively, and it contains a 1589-bp full-length cDNA that encodes a protein containing 403 amino acids. In this study, the pepper gene CaBPM4 (Capsicum annuum BTB-POZ and MATH domain protein) was cloned, and its role in responses to Phytophthora capsici, cold, drought, and salt stress were characterized. The results of quantitative RT-PCR revealed that CaBPM4 was down-regulated under P. capsici infection, salicylic acid, H 2 O 2 , and abscisic acid treatments, while abiotic stresses, including salt, cold, and drought, enhanced its transcript level. Furthermore, CaBPM4 silencing significantly impaired resistance to P. capsici, apparently by altering the transcript level of defense-related genes CaPR1, CaDEF1, and CaSAR82 and reducing root activity. However, CaBPM4-silenced plants exhibited remarkably increased peroxidase activity and decreased malondialdehyde concentrations, indicating that CaBPM4 may enhance resistance to salt and drought stress. Further study should focus on the mechanism by which CaBPM4 regulates the defense response to P. capsici infection and abiotic stresses.Broad-complex Tramtrack and Bric-a-brac (BTB) domain, also known as POX virus and Zinc finger (POZ) domain, is evolutionarily conserved and reported to be involved in transcriptional regulation [14,15] and targeting proteins for degradation [16]. BTB domain was first discovered in Drosophila [15], and since then, its vital roles in hormone-induced signaling transduction and plant responses to biotic and abiotic stresses have been revealed [17,18]. For example, in soybean, GmBTB expression was induced strongly by Phytophthora sojae infection [19]. The potato (Solanum tuberosum) BTB-containing protein NRL1 can interact with the positive immunity regulator NbSWAP70 in Nicotiana benthamiana and also suppress plant immunity against Phytophthora infestans infections [20]. Expression of Arabidopsis NPR1 in wheat, transgenic tobacco, rice, and tomato enhanced plant resistance to pathogen attacks [21][22][23][24]. Furthermore, the BTB-containing protein ARIA also affected the abscisic acid (ABA)-mediated signaling pathway through interactions with a transcription factor ABF2 [25]. The BTB domain-containing protein gene family of tomato was also well-known to be related closely to abiotic stresses [26].Many BTB/POZ proteins contain a secondary protein domain, such as a meprin and TRAF (tumor necrosis factor receptor associated factor) homology (MATH) domain, a tumor necrosis factor receptor-associated element [27]. A previous study suggested that MATH domain-containing proteins positively regulate defense responses to abiotic stresses in Arabidopsis and rice [28]. Different functional roles have been identified for BTB proteins, including transcription repression, cytoskeleton regulation, and prote...

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Section: Discussionsupporting

confidence: 68%

Contribution of CaBPM4, a BTB Domain–Containing Gene, to the Response of Pepper to Phytophthora capsici Infection and Abiotic Stresses

Liu

Zhang

et al. 2019

Agronomy

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“…Apart from the TGAs, several bZIPs from other subfamilies were also involved in disease resistance. For example, CabZIP2 and CabZIP63 were found to enhance the resistance to P. syringae and R. solanacearum in pepper (Lim et al, 2015;Shen et al, 2016), LrbZIP1 was involved in defense responses against Fusarium oxysporum in Lilium (Zhang et al, 2014), and bZIP10 modulates basal defense and cell death following infection in Arabidopsis (Kaminaka et al, 2006). In this study, we found that StbZIP61 functioned in defense against P. infestans.…”

Section: Discussionmentioning

confidence: 55%

“…syringae and R . solanacearum in pepper (Lim et al ., ; Shen et al ., ), LrbZIP1 was involved in defense responses against Fusarium oxysporum in Lilium (Zhang et al ., ), and bZIP10 modulates basal defense and cell death following infection in Arabidopsis (Kaminaka et al ., ). In this study, we found that StbZIP61 functioned in defense against P .…”

Section: Discussionmentioning

confidence: 99%

The potato transcription factor StbZIP61 regulates dynamic biosynthesis of salicylic acid in defense against Phytophthora infestans infection

et al. 2018

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Salicylic acid (SA) signalling plays an essential role in plant innate immunity. In this study, we identified a component in the SA signaling pathway in potato (Solanum tuberosum), the transcription factor StbZIP61, and characterized its function in defence against Phytophthora infestans. Expression of StbZIP61 was induced upon P. infestans infection and following exposure to the defense signaling hormones SA, ethylene and jasmonic acid. Overexpression of StbZIP61 increased the tolerance of potato plants to P. infestans while RNA interference (RNAi) increased susceptibility. Yeast two-hybrid and pull down experiments revealed that StbZIP61 could interact with an NPR3-like protein (StNPR3L) that inhibited its DNA-binding and transcriptional activation activities. Moreover, StNPR3L interacted with StbZIP61 in an SA-dependent manner. Among candidate genes involved in SA-regulated defense responses, StbZIP61 had a significant impact on expression of StICS1, which encodes a key enzyme for SA biosynthesis. StICS1 transcription was induced upon P. infestans infection and this responsive expression to the pathogen was reduced in StbZIP61 RNAi plants. Accordingly, StICS1 expression was remarkably enhanced in StbZIP61-overexpressing plants. Together, our data demonstrate that StbZIP61 functions in concert with StNPR3L to regulate the temporal activation of SA biosynthesis, which contributes to SA-mediated immunity against P. infestans infection in potato.

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“…Pepper bZIP TF, CaAIBZ1, interacting with and degraded by CaASRF1 (C. annuum ABA-Sensitive RING Finger E3 ligase 1), positively modulated ABA signaling and the drought stress (Joo et al, 2018). Additionally, CabZIP63 was up-regulated by Ralstonia solanacearum inoculation at high temperature-high humidity and acted as a positive regulator in this process by regulating the expression of CaWRKY40 (Shen et al, 2016). Collectively, the bZIP TFs family in pepper is an important gene family involved in stress response along with a crucial role in growth and development.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the bZIP Transcription Factor Family in Pepper (Capsicum annuum L.): CabZIP25 Positively Modulates the Salt Tolerance

Gai

Qiao

et al. 2020

Front. Plant Sci.

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The basic leucine zipper (bZIP) proteins compose a family of transcription factors (TFs), which play a crucial role in plant growth, development, and abiotic and biotic stress responses. However, no comprehensive analysis of bZIP family has been reported in pepper (Capsicum annuum L.). In this study, we identified and characterized 60 bZIP TFencoding genes from two pepper genomes. These genes were divided into 10 groups based on their phylogenetic relationships with bZIP genes from Arabidopsis. Six introns/ exons structural patterns within the basic and hinge regions and the conserved motifs were identified among all the pepper bZIP proteins, on the basis of which, we classify them into different subfamilies. Based on the transcriptomic data of Zunla-1 genome, expression analyses of 59 pepper bZIP genes (not including CabZIP25 of CM334 genome), indicated that the pepper bZIP genes were differentially expressed in the pepper tissues and developmental stages, and many of the pepper bZIP genes might be involved in responses to various abiotic stresses and phytohormones. Further, gene expression analysis, using quantitative real-time PCR (qRT-PCR), showed that the CabZIP25 gene was expressed at relatively higher levels in vegetative tissues, and was strongly induced by abiotic stresses and phytohormones. In comparing with wild type Arabidopsis, germination rate, fresh weight, chlorophyll content, and root lengths increased in the CabZIP25-overexpressing Arabidopsis under salt stress. Additionally, CabZIP25-silenced pepper showed lower chlorophyll content than the control plants under salt stress. These results suggested that CabZIP25 improved salt tolerance in plants. Taken together, our results provide new opportunities for the functional characterization of bZIP TFs in pepper.

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Pepper CabZIP63 acts as a positive regulator duringRalstonia solanacearumor high temperature–high humidity challenge in a positive feedback loop with CaWRKY40

Abstract: HighlightCabZIP63, indirectly activated by CaWRKY40, positively modulates transcription of CabZIP63 and CaWRKY40, enhances the binding of CaWRKY40 to its target promoters, and, therefore, increases resistance to Ralstonia solanacearum and thermotolerance.

Cited by 74 publications

References 89 publications

Contribution of CaBPM4, a BTB Domain–Containing Gene, to the Response of Pepper to Phytophthora capsici Infection and Abiotic Stresses

Contribution of CaBPM4, a BTB Domain–Containing Gene, to the Response of Pepper to Phytophthora capsici Infection and Abiotic Stresses

The potato transcription factor StbZIP61 regulates dynamic biosynthesis of salicylic acid in defense against Phytophthora infestans infection

Characterization of the bZIP Transcription Factor Family in Pepper (Capsicum annuum L.): CabZIP25 Positively Modulates the Salt Tolerance

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