Pepper (<i>Capsicum annuum</i>) Plants Harboring the Begomovirus Resistance Gene <i>pepy-1</i> Show Delayed Symptom Progress and High Productivity Under Natural Field Conditions

Abstract

Pepper yellow leaf curl disease (PepYLCD) caused by begomoviruses is one of the most devastating diseases affecting pepper (Capsicum spp.) production worldwide. In our previous study, a loss-of-function allele, pepy-1, encoding messenger RNA surveillance factor Pelota was identified as a begomovirus resistance gene from a C. annuum cultivar BaPep-5. In this study, to investigate the effectiveness of pepy-1 conferred resistance against begomovirus in the field, we conducted a three-year evaluation under natural field conditions in Indonesia. The lowest PepYLCD incidence and significantly higher fruit productivity were observed in BaPep-5 when compared to six other commonly cultivated pepper cultivars. The subsequent comparison between BaPep-5 and the susceptible BaPep-4 showed that pepy-1 slowed down the disease onset and progression, resulting in a higher fruit productivity trait in the field. Multiple comparison analyses using an F 2 population obtained by crossing BaPep-5 with BaPep-4 showed that the pepy-1 homozygous individuals had significantly higher fruit productivity, twice than those of the Pepy-1 homozygous or heterozygous individuals. In conclusion, the introgression of pepy-1 is effective to reduce the economic loss of pepper fruit production under natural field infection of begomoviruses.

Characteristics of Virus Symptoms in Chili Plants (Capsicum frutescens) Based on RGB Image Analysis

“…Virus detection using the TBIA method showed that TMV and CMV were the most dominant viruses infecting chili plants in the field, either occurring as a single or mixed infection with other viruses (PepMoV and ChiVMV). These results complement to previous studies which reported various types of viruses associated with chili plants in Indonesia, such as Pepper yellow leaf curl Indonesia virus (PYLCIV) and Pepper yellow leaf curl Aceh virus (PYLCAV) (Hidayat, Haryadi, & Nurhayati, 2009;Kesumawati et al, 2019;Pohan et al, 2022), Polerovirus (Suastika, Hartono, Nyana, & Natsuaki, 2012), Potato virus Y (PVY) (Damiri, 2014), CMV and ChiVMV (Subekti, Hidayat, Nurhayati, & Sujiprihati, 2006), as well as TMV (Taufik, Khaeruni, & Rombe, 2011).…”

Growth and yield of some genotypes of pepper (Capsicum annuum L.) in response to begomovirus resistance

“…[12] had been identified that C. annuum cultivar Perintis (BaPep-5) is a recessive resistance gene pepy-1 in peppers in controlled conditions of laboratory, this gene was effective against the bipartite begomoviruses PepYLCAV and PepYLCAV. Moreover, [13] showed that Perintis (BaPep-5) significantly higher fruit productivity compared to six other commonly cultivated pepper cultivars included Kencana (BaPep-4), and Perintis (BaPep-5) had the lowest PepYLCD incidence. State that the estimation of yield variables character character can be used as a consideration for efficient selection.…”