Peppermint (<i>Mentha piperita L</i>.) extract effectively inhibits cytochrome P450 3A4 (CYP3A4) mRNA induction in rifampicin-treated HepG2 cells

Kobayashi, Tsukasa; Sugaya, Kouichi; Onose, Jun‐ichi; Abe, Naoki

doi:10.1080/09168451.2019.1608802

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…The results showed that epimedium inhibited CYP3A4 transcription without any cytotoxicity. Dexamethasone alone at a low concentration of 10 μM induced CYP3A4 mRNA, similar to rifampin (a CYP3A4 enhancer ( Kobayashi et al, 2019 )). However, in combination with epimedium, we observed 50% less suppression of CYP3A4 mRNA expression.…”

Section: Discussionmentioning

confidence: 96%

Cytochrome P450 3A4 suppression by epimedium and active compound kaempferol leads to synergistic anti-inflammatory effect with corticosteroid

Maskey

et al. 2023

Front. Pharmacol.

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Introduction: Cytochrome P450 (CYP) 3A4 is a major drug metabolizing enzyme for corticosteroids (CS). Epimedium has been used for asthma and variety of inflammatory conditions with or without CS. It is unknown whether epimedium has an effect on CYP 3A4 and how it interacts with CS. We sought to determine the effects of epimedium on CYP3A4 and whether it affects the anti-inflammatory function of CS and identify the active compound responsible for this effect.Methods: The effect of epimedium on CYP3A4 activity was evaluated using the Vivid CYP high-throughput screening kit. CYP3A4 mRNA expression was determined in human hepatocyte carcinoma (HepG2) cells with or without epimedium, dexamethasone, rifampin, and ketoconazole. TNF-α levels were determined following co-culture of epimedium with dexamethasone in a murine macrophage cell line (Raw 264.7). Active compound (s) derived from epimedium were tested on IL-8 and TNF-α production with or without corticosteroid, on CYP3A4 function and binding affinity.Results: Epimedium inhibited CYP3A4 activity in a dose-dependent manner. Dexamethasone enhanced the expression of CYP3A4 mRNA, while epimedium inhibited the expression of CYP3A4 mRNA and further suppressed dexamethasone enhancement of CYP3A4 mRNA expression in HepG2 cells (p < 0.05). Epimedium and dexamethasone synergistically suppressed TNF-α production by RAW cells (p < 0.001). Eleven epimedium compounds were screened by TCMSP. Among the compounds identified and tested only kaempferol significantly inhibited IL-8 production in a dose dependent manner without any cell cytotoxicity (p < 0.01). Kaempferol in combination with dexamethasone showed complete elimination of TNF-α production (p < 0.001). Furthermore, kaempferol showed a dose dependent inhibition of CYP3A4 activity. Computer docking analysis showed that kaempferol significantly inhibited the catalytic activity of CYP3A4 with a binding affinity of −44.73kJ/mol.Discussion: Inhibition of CYP3A4 function by epimedium and its active compound kaempferol leads to enhancement of CS anti-inflammatory effect.

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Section: Discussionmentioning

confidence: 96%

Cytochrome P450 3A4 suppression by epimedium and active compound kaempferol leads to synergistic anti-inflammatory effect with corticosteroid

Maskey

et al. 2023

Front. Pharmacol.

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“…Peppermint tea inhibits CYP3A4 induction by rifampicin in vitro [95]; oil: contradicting results on CYP inhibition in vitro [96,97]; enhanced cyclosporine bioavailability in rats [98] Cyclosporine, tacrolimus?…”

Section: Peppermintmentioning

confidence: 99%

Herb–Drug Interaction in Inflammatory Diseases: Review of Phytomedicine and Herbal Supplements

Lippert

Renner

2022

JCM

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Many people worldwide use plant preparations for medicinal purposes. Even in industrialized regions, such as Europe, where conventional therapies are accessible for the majority of patients, there is a growing interest in and usage of phytomedicine. Plant preparations are not only used as alternative treatment, but also combined with conventional drugs. These combinations deserve careful contemplation, as the complex mixtures of bioactive substances in plants show a potential for interactions. Induction of CYP enzymes and pGP by St John’s wort may be the most famous example, but there is much more to consider. In this review, we shed light on what is known about the interactions between botanicals and drugs, in order to make practitioners aware of potential drug-related problems. The main focus of the article is the treatment of inflammatory diseases, accompanied by plant preparations used in Europe. Several of the drugs we discuss here, as basal medication in chronic inflammatory diseases (e.g., methotrexate, janus kinase inhibitors), are also used as oral tumor therapeutics.

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Plant-based foods and hepatocellular carcinoma: A review on mechanistic understanding

Saeed

Maqsood

Saeed

et al. 2022

Critical Reviews in Food Science and Nutrition

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Peppermint (Mentha piperita L.) extract effectively inhibits cytochrome P450 3A4 (CYP3A4) mRNA induction in rifampicin-treated HepG2 cells

Cited by 4 publications

References 22 publications

Cytochrome P450 3A4 suppression by epimedium and active compound kaempferol leads to synergistic anti-inflammatory effect with corticosteroid

Cytochrome P450 3A4 suppression by epimedium and active compound kaempferol leads to synergistic anti-inflammatory effect with corticosteroid

Herb–Drug Interaction in Inflammatory Diseases: Review of Phytomedicine and Herbal Supplements

Plant-based foods and hepatocellular carcinoma: A review on mechanistic understanding

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