Pepsin−Gold Colloid Conjugates:  Preparation, Characterization, and Enzymatic Activity

Gole, Anand; Dash, Chandravanu; Ramakrishnan, Vidya; Sainkar, S. R.; Mandale, A.B.; Rao, M. Narayana; Sastry, Murali

doi:10.1021/la001164w

Cited by 551 publications

(326 citation statements)

References 39 publications

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“…when the pH is close to the pI of the protein or the nanoparticle so that the electrostatic repulsion is reduced. After adsorption, the protein can be irreversibly immobilized by those forces or a combination of them (Gole et al 2001). Potentially, the protein can get into intimate contact with the particle surface by partial or complete denaturation (Gao et al 2002), giving rise e.g.…”

Section: (Iii) Peptides Proteins Enzymes and Antibodiesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: (Iii) Peptides Proteins Enzymes and Antibodiesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…The overall observation confirms the presence of protein in the samples of silver nanoparticles. It is reported earlier that proteins can bind to nanoparticles either through free amine groups or cysteine residues in the proteins (Kittel 1992;Gole et al 2001). IR spectroscopic study confirmed that the carbonyl group from amino acid residue and proteins has the strongest ability to bind metal indicating the proteins could possibly form a layer covering the metal nanoparticles (i.e., capping of AgNps) to prevent agglomeration and thereby stabilize the medium.…”

Section: Resultsmentioning

confidence: 71%

Biosynthesis of silver nanoparticles from Premna serratifolia L. leaf and its anticancer activity in CCl4-induced hepato-cancerous Swiss albino mice

Paul¹,

Selvi²,

Karmegam³

2015

Appl Nanosci

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In this study, we report the biosynthesis of silver nanoparticles using the ethanolic leaf powder extract of Premna serratifolia L. and its anticancer activity in carbon tetra chloride (CCl 4 )-induced liver cancer in Swiss albino mice (Balb/c). The synthesized silver nanoparticles were characterized by SEM, FTIR and XRD analyses. The Debye-Scherrer equation was used to calculate particle size and the average size of silver nanoparticles synthesized from P. serratifolia leaf extract was 22.97 nm. The typical pattern revealed that the sample contained cubic structure of silver nanoparticles. FTIR analysis confirmed that the bioreduction of silver ions to silver nanoparticles is due to reduction by capping material of the plant extract. The silver nanoparticles of P. serratifolia leaf extract were effective in treating liver cancer in Swiss albino mice when compared with P. serratifolia leaf extract with isoleucine.

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“…[28][29][30][31]. It has been proposed that free amine groups of proteins bind to AuNP resulting in its stabilization [32]. Also, cell surface bound proteins are responsible for reduction and stabilization of AuNP [31].…”

Section: Ftir Analysismentioning

confidence: 99%

Kinetics of Synthesis of Gold Nanoparticles by Acinetobacter sp. SW30 Isolated from Environment

et al. 2016

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Cell biomass and metal salt concentration have great influence on morphology of biosynthesized nanoparticle. The aim of present study was to evaluate the effect of varying cell density and gold salt concentrations on synthesis of nanoparticles and its morphology, which has not been studied in bacteria till now. When cells of Acinetobacter sp. SW30 were incubated with different cell density and gold chloride concentrations, tremendous variation in color of colloidal solution containing gold nanoparticles (AuNP) was observed indicating variation in their size and shapes. Surprisingly, monodispersed spherical AuNP of size *19 nm were observed at lowest cell density and HAuCl 4 salt concentration while increase in cell number resulted in formation of polyhedral AuNP (*39 nm). Significance of this study lays in the fact that the shape and dispersity of AuNP can be customized depending up on the requirement. FTIR spectrum revealed shift from 3221 to 3196 cm -1 indicating the presence and role of amino acids in Au 3? reduction while possible involvement of amide I and II groups in stabilization of AuNP. The rate constant was calculated for cell suspension of 2.1 9 10 9 cfu/ml challenged with 1.0 mM HAuCl 4 , incubated at 30°C and pH 7 using the slopes of initial part of the plot log (A a -A t ) versus time as 1.99 9 10 -8 M.Also, this is the first study to report the kinetics of gold nanoparticle synthesis by Acinetobacter sp. SW30.

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Pepsin−Gold Colloid Conjugates: Preparation, Characterization, and Enzymatic Activity

Cited by 551 publications

References 39 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Biosynthesis of silver nanoparticles from Premna serratifolia L. leaf and its anticancer activity in CCl4-induced hepato-cancerous Swiss albino mice

Kinetics of Synthesis of Gold Nanoparticles by Acinetobacter sp. SW30 Isolated from Environment

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