Electricity generation is tied to various environmental and social consequences. In prior studies, the environmental emissions associated with electricity generation were calculated using average emission factors (AEFs) whose use is different from the method of marginal emission factors (MEFs) in regard to the geographical redefinition and new policies applied to the US electricity grid in 2013. Moreover, the amount of emissions being released at a generation site depends on the technology of the generating units; it is important to take into account this factor as well. Thus, this paper provides comparisons between different historical and real-time approaches of estimating MEFs (i.e., CO2, SO2, and NOx) for the Midcontinent Independent System Operator (MISO) electricity region. The region under study is the same for all the scenarios, although the comparative time frames are different. The study is focused on the similarities observed in the data trends and system behaviors. We carry out different temporal comparisons whose results show the value of real-time approaches for estimating the MEFs for each location and at any time. These approaches can be extended to other regions to assist with proper investment and policy making, thereby increasing the grid efficiency, mitigating the environmental emissions, and clarifying the byproducts of energy consumption.