Peptide Amphiphiles and Porous Biodegradable Scaffolds for Tissue Regeneration in the Brain and Spinal Cord

Ellis-Behnke, Rutledge; Schneider, Gerald

doi:10.1007/978-1-61779-052-2_17

Cited by 20 publications

(16 citation statements)

References 55 publications

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“…Other researchers are working on customizing treatments, such as nanoparticles that match the unique genetic profile of a patient’s cancer cells in order to seek out and destroy them. 5–7 More than 60 drugs and drug delivery systems based on nanotechnology, and more than 90 medical devices or diagnostic tests, such as amphiphiles that regenerate injured nerve cells, 8 are already being tested. Neuroscience applications of nanotechnology involve investigating molecular, cellular, and physiological processes and research aimed at limiting and reversing neuropathological diseases.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Neurotoxicity Resulting from Exposure of Cultured Neural Cells to Several Types of Nanoparticles

et al. 2017

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Laboratory and industrial production of various nanoparticles, single-walled nanotubes (SWNTs), fullerene (C60), cadmium selenide (CdSe) quantum dots, carbon black (CB), and dye-doped silica nanospheres (NSs), has greatly increased in the past 15 years. However, little research has been done to analyze the toxicity of these materials. With recent studies showing that nano-substances can cross the blood–brain barrier, we examined the neurotoxicity of these manufactured nanoparticles. By employing the rat PC-12 neuronal-like cell line as the basis for our studies, we were able to evaluate the toxicity caused by these five nanoparticles. The level of toxicity was measured by testing for cell viability using the lactate dehydrogenase (LDH) cell viability assay, morphological analysis of changes in cellular structures, and Western blot analyses of αII-spectrin breakdown products (SBDP) as cell death indicators. Our results showed cytotoxicity in nondifferentiated PC-12 cells exposed to CB (10–100 µg/mL), SWNTs (10–100 µg/mL), C60 (100 µg/mL), CdSe (10 µg/mL), CB (500 µg/mL), and dye-doped silicon NSs (10 µg/mL). Exposure to higher concentrations (100 µg/mL) of SWNTs, CB, and C60 increased the formation of SBDP150/145, as well as cell membrane contraction and the formation of cytosolic vacuoles. The incorporations of the nanoparticles into cell cytoplasm were observed using the fluorescent dye-doped NSs in both nondifferentiated and nerve growth factor (NGF)-differentiated PC-12 cells. When PC-12 cells are differentiated, they appeared to be even more sensitive to cytotoxicity of nanoparticles such as CB 10 nm (10–100 µg/mL), CB 100 nm (10–100 µg/mL), and CdSe (1–10 µg/mL).

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Section: Introductionmentioning

confidence: 99%

In Vitro Neurotoxicity Resulting from Exposure of Cultured Neural Cells to Several Types of Nanoparticles

et al. 2017

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“…The benefits of self-assembling peptides over some natural and/or synthetic materials are that: (i) selfassembling peptide scaffold is a molecular designed bioactive matrix without exogenous proteins, posing a minimized risk of carrying biological pathogens or contaminants, thus appears to be immunologically inert; (ii) liquid self assembling peptide scaffold can be injected directly into the lesion site and fill the cavities, regardless of their size and shape; (iii) self-assembling peptides are amendable to functionalization to mimic the naturally occurring proteins; (iv) self-assembling peptides allow for high cell implantation densities and show highly potential for controlled drug release [32,[85][86][87]. The use of selfassembling peptide nanofiber scaffolds for CNS is a novel approach by means of a synthetic biological nanofiber hydrogel that fills the cavities and links the damaged nerve segments of the lesion.…”

Section: Traditional Nanofiber Biomaterials For Cnsmentioning

confidence: 99%

“…2.2 Self-assembling peptide nanofiber hydrogel scaffolds Peptide nanofiber scaffold prepared via self-assembling process is an area of growing interest for CNS regenerative medicine [32,63]. Self-assembly is one of the most powerful ways to prepare nanostructure materials and offers great opportunities for the creation of novel biomaterials [64][65][66][67][68][69][70].…”

Section: Traditional Nanofiber Biomaterials For Cnsmentioning

confidence: 99%

“…Acting as a substitute of extracellular matrix (ECM), biomaterial scaffold is a crucial structure component for supporting cells and provides a permissive microenvironment that influences the function of cells [32]. It can also be used to deliver drugs at a rate designed to match the physiological need of the tissue [33][34].…”

Section: Nanofiber Biomaterials For Cns Regenerationmentioning

confidence: 99%

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Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Liu

Wang

et al. 2014

Front. Mater. Sci.

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Central nervous system (CNS) presents a complex regeneration problem due to the inability of central neurons to regenerate correct axonal and dendritic connections. However, recent advances in developmental neurobiology, cell signaling, cell-matrix interaction, and biomaterials technologies have forced a reconsideration of CNS regeneration potentials from the viewpoint of tissue engineering and regenerative medicine. The applications of a novel tissue regeneration-inducing biomaterial and stem cells are thought to be critical for the mission. The use of peptide nanofiber hydrogels in cell therapy and tissue engineering offers promising perspectives for CNS regeneration. Self-assembling peptide undergo a rapid transformation from liquid to gel upon addition of counterions or pH adjustment, directly integrating with the host tissue. The peptide nanofiber hydrogels have mechanical properties that closely match the native central nervous extracellular matrix, which could enhance axonal growth. Such materials can provide an optimal three dimensional microenvironment for encapsulated cells. These materials can also be tailored with bioactive motifs to modulate the wound environment and enhance regeneration. This review intends to detail the recent status of selfassembling peptide nanofiber hydrogels for CNS regeneration.

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“…There have been several studies that have concentrated on the microstructural design of porous scaffolds, which must be conditioned in vivo prior to implantation (49,50). However, this has the major disadvantage of increasing the difficulty of the design at the engineering level and surgical implantation may also be challenging.…”

Section: Scaffoldmentioning

confidence: 99%

Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

Zhou

et al. 2013

Experimental and Therapeutic Medicine

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Spinal cord injury (SCI) may lead to a devastating and permanent loss of neurological function, which may place a great economic burden on the family of the patient and society. Methods for reducing the death of neuronal cells, inhibiting immune and inflammatory reactions, and promoting the growth of axons in order to build up synapses with the target cells are the focus of current research. Target cells are located in the damaged spinal cord which create a connect with the scaffold. As tissue engineering technology is developed for use in a variety of different areas, particularly the biomedical field, a clear understanding of the mechanisms of tissue engineering is important. This review establishes how this technology may be used in basic experiments with regard to SCI and considers its potential future clinical use.

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Peptide Amphiphiles and Porous Biodegradable Scaffolds for Tissue Regeneration in the Brain and Spinal Cord

Cited by 20 publications

References 55 publications

In Vitro Neurotoxicity Resulting from Exposure of Cultured Neural Cells to Several Types of Nanoparticles

In Vitro Neurotoxicity Resulting from Exposure of Cultured Neural Cells to Several Types of Nanoparticles

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

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