Peptide-Assembled Graphene Oxide as a Fluorescent Turn-On Sensor for Lipopolysaccharide (Endotoxin) Detection

Lim, Seng Koon; Chen, Peng; Lee, Fook Loy; Moochhala, Shabbir; Liedberg, Bo

doi:10.1021/acs.analchem.5b02270

Cited by 102 publications

(66 citation statements)

References 44 publications

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“…31 As shown in Fig. 3b, the peptide motif KLVFF (Aβ [16][17][18][19][20] self-assembled into 2D membranes under the influence of hydrated electrons coming from argon glow discharge. Meanwhile, the metal ions in solution could be simultaneously reduced to MNPs (Au, Pt, Ag and Pd) by these high-energy electrons.…”

Section: Peptide and Noble Mnp Nanohybridsmentioning

confidence: 91%

“…4b, a synthetic peptide-GO assembly can serve as a fluorescence turn-on sensor. 20 To be specific, lipopolysaccharide-binding peptide physically adsorbs to the negatively charged GO via electrostatic interactions and π-π stacking, resulting in fluorescence quenching. The fluorescence signal would recover when lipopolysaccharide, major component in the outer cell membrane of Gram-negative bacteria, totally binds to the peptide, releasing dye-labeled peptide from the GO sheets.…”

Section: 22mentioning

confidence: 99%

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Design, fabrication, and biomedical applications of bioinspired peptide–inorganic nanomaterial hybrids

Wang

et al. 2017

J. Mater. Chem. B

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By specifically binding with various inorganic nanomaterials through their functional groups or introducing a special reactivity, the nanostructures and biofunctions of designed peptides are able to be enriched. This review provides a brief discussion on the design, composition and biomedical applications of bioinspired peptide-inorganic nanomaterial hybrids. The attaching mechanism of different peptide-inorganic nanomaterials are discussed from the view of the functional group, nanostructure, and conformational freedom. Particularly, the structures and functions of the obtained hybrids based on different nanomaterials are described in detail. In addition, we highlight some examples of self-assembled peptide-inorganic nanomaterial systems with relevance to biomedical applications including biosensors, cell targeting, bioimaging, biomineralization, biocatalyst, drug delivery and others. We also give a short outlook on the broad prospect of fabrication and applications of peptide-inorganic nanomaterial hybrids.

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Section: Peptide and Noble Mnp Nanohybridsmentioning

confidence: 91%

Section: 22mentioning

confidence: 99%

Design, fabrication, and biomedical applications of bioinspired peptide–inorganic nanomaterial hybrids

Wang

et al. 2017

J. Mater. Chem. B

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“…The light emitted by these dyes is quenched by graphene when the two systems are coupled while dye emission becomes visible when the coupling is broken. This scheme was utilized to detect DNA folding or interactions with specific complexes [209], or to detect particular substances which can interact directly with the dye molecules [210].…”

Section: Graphite Graphitic Carbons and Graphenementioning

confidence: 99%

The Role of Functionalization in the Applications of Carbon Materials: An Overview

Speranza

2019

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The carbon-based materials (CbMs) refer to a class of substances in which the carbon atoms can assume different hybridization states (sp 1 , sp 2 , sp 3 ) leading to different allotropic structures -. In these substances, the carbon atoms can form robust covalent bonds with other carbon atoms or with a vast class of metallic and non-metallic elements, giving rise to an enormous number of compounds from small molecules to long chains to solids. This is one of the reasons why the carbon chemistry is at the basis of the organic chemistry and the biochemistry from which life on earth was born. In this context, the surface chemistry assumes a substantial role dictating the physical and chemical properties of the carbon-based materials. Different functionalities are obtained by bonding carbon atoms with heteroatoms (mainly oxygen, nitrogen, sulfur) determining a certain reactivity of the compound which otherwise is rather weak. This holds for classic materials such as the diamond, the graphite, the carbon black and the porous carbon but functionalization is widely applied also to the carbon nanostructures which came at play mainly in the last two decades. As a matter of fact, nowadays, in addition to fabrication of nano and porous structures, the functionalization of CbMs is at the basis of a number of applications as catalysis, energy conversion, sensing, biomedicine, adsorption etc. This work is dedicated to the modification of the surface chemistry reviewing the different approaches also considering the different macro and nano allotropic forms of carbon.C 2019, 5, 84 3 of 45 narrow graphite-like interconnected layers. This leads to a closed pore rigid structure that is chemically inert, hard but brittle [48][49][50].Due to the highly resistant, conductive, and inert network, glassy carbons are utilized in a series of rather different applications. They are utilized as electrodes in solid state batteries and in industrial harsh chemical processes where also high temperatures are involved such as crystallization of CaF2, CdS and ZnS. Glassy carbon can be utilized to manufacture high temperature furnace elements. Due to the chemical inertness, glassy carbons are utilized also in fabrication of protheses. Porous carbon and carbon black are much softer. Generally, in industrial applications important is the extension of the surface which is exposed to the external environment. The porous carbon is characterized by a high specific surface area enhancing the interaction with the external medium. Although it possesses a lower conductivity with respect to glassy carbon, the high porosity and the presence of active sites makes it a good material to fabricate electrodes for applications in electrochemistry [51][52][53] and bioelectrodes for sensing and stimulation [54,55].The same holds for the carbon black mainly utilized as additive in rubbers and polymers to improve their mechanical properties, or as a pigment [56] although new potentialities have been recently envisaged [57].Common to all the forms of carbon which are bri...

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“…Enzymatic limulus amoebocyte lysate assay is commonly used to detect LPS, but this method is susceptible to variations in experimental conditions, particularly pH and temperature, and requires complicated sample preparation and testing procedures. [16][17][18] To address these drawbacks, scholars have developed alternative methods for LPS detection by using various signals, such as colorimetric, [19][20][21][22] surface-enhanced Raman scattering, 23 uorescence, 24 and electrochemical signals. [25][26][27] These methods exhibit high sensitivity and accuracy.…”

Section: 15mentioning

confidence: 99%

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

et al. 2017

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Bacterial toxin contamination is a serious food safety issue. Rapid, facile detection of bacterial toxins in food is crucial to control their harmful effects on human health. In this study, a facile, selective, and cost-effective method with a 6-carboxy-X-rhodamine labeled lipopolysaccharide binding aptamer (ROX-LBA)/GO fluorescent probe was developed based on the specific recognition characteristics of aptamers and the super fluorescence quenching efficiency of graphene oxide (GO) and used for the detection of lipopolysaccharides (LPS). ROX-LBA is adsorbed on the GO surface because of its strong p-p stacking interactions in the flexible single-stranded state, and fluorescence is quenched by GO via Förster resonance energy transfer (FRET). After adding LPS, the aptamer binds to LPS and forms a LPS/ROX-LBA complex, which exhibits weak binding affinity with GO. ROX-LBA cannot effectively combine with GO, and fluorescence is restored. The concentration of LPS can be quantitatively analyzed by observing fluorescence changes in the sensing system. The fluorescence intensity proportionally increases with LPS concentration (25-1600 ng mL À1 ), and the detection limit is 15.7 ng mL À1 . Hence, the proposed method provides an alternative strategy for the specific detection of endotoxin in drink samples (e.g., apple juice and beer) and could be widely applied to quantify various bacterial toxins by replacing the aptamer sequences.

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Peptide-Assembled Graphene Oxide as a Fluorescent Turn-On Sensor for Lipopolysaccharide (Endotoxin) Detection

Cited by 102 publications

References 44 publications

Design, fabrication, and biomedical applications of bioinspired peptide–inorganic nanomaterial hybrids

Design, fabrication, and biomedical applications of bioinspired peptide–inorganic nanomaterial hybrids

The Role of Functionalization in the Applications of Carbon Materials: An Overview

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

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