Peptide‐Based Nanocarriers for Intracellular Delivery of Biologically Active Proteins

Lo, Seong Loong; Wang, Shu

doi:10.1002/9780470875780.ch18

Cited by 2 publications

(3 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CPPs have been reported to enhance the permeability of various macromolecules, including proteins [148–150]. Early studies showed that the TAT peptide can mediate the translocation of covalently coupled proteins [151, 152].…”

Section: Approaches To Design and Improve Membrane Permeabilitymentioning

confidence: 99%

Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Yang

Hinner²

2014

Methods in Molecular Biology

662

493

View full text Add to dashboard Cite

The ability to efficiently access cytosolic proteins is desired in both biological research and medicine. However, targeting intracellular proteins is often challenging, because to reach the cytosol, exogenous molecules must first traverse the cell membrane. This review provides a broad overview of how certain molecules are thought to cross this barrier, and what kinds of approaches are being made to enhance the intracellular delivery of those that are impermeable. We first discuss rules that govern the passive permeability of small molecules across the lipid membrane, and mechanisms of membrane transport that have evolved in nature for certain metabolites, peptides, and proteins. Then, we introduce design strategies that have emerged in the development of small molecules and peptides with improved permeability. Finally, intracellular delivery systems that have been engineered for protein payloads are surveyed. Viewpoints from varying disciplines have been brought together to provide a cohesive overview of how the membrane barrier is being overcome.

show abstract

Section: Approaches To Design and Improve Membrane Permeabilitymentioning

confidence: 99%

Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Yang

Hinner²

2014

Methods in Molecular Biology

662

493

View full text Add to dashboard Cite

show abstract

“…While much progress has been made in the development of delivery systems that utilize mechanical disruption 1,2 or various materials, 3–8 their practical implementation remains a significant challenge. 9 …”

Section: Introductionmentioning

confidence: 99%

“…The ability to safely and efficiently deliver exogenous proteins to the cytoplasm of target cells is highly desired for allowing potential therapeutic interventions. While much progress has been made in the development of delivery systems that utilize mechanical disruption , or various materials, − their practical implementation remains a significant challenge …”

Section: Introductionmentioning

confidence: 99%

Antibody-Mediated Neutralization of Perfringolysin O for Intracellular Protein Delivery

et al. 2015

View full text Add to dashboard Cite

Perfringolysin O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial pore-forming proteins, which are highly efficient in delivering exogenous proteins to the cytoplasm. However, the indiscriminate and potent cytotoxicity of PFO limits its practical use as an intracellular delivery system. In this study, we describe the design and engineering of a bispecific, neutralizing antibody against PFO, which targets reversibly attenuated PFO to endocytic compartments via receptor-mediated internalization. This PFO-based system efficiently mediated the endosomal release of a co-targeted gelonin construct with high specificity and minimal toxicity in vitro. Consequently, the therapeutic window of PFO was improved by more than 5 orders of magnitude. Our results demonstrating that the activity of pore-forming proteins can be controlled by antibody-mediated neutralization present a novel strategy for utilizing these potent membrane-lytic agents as a safe and effective intracellular delivery vehicle.

show abstract

Peptide‐Based Nanocarriers for Intracellular Delivery of Biologically Active Proteins

Cited by 2 publications

References 71 publications

Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Antibody-Mediated Neutralization of Perfringolysin O for Intracellular Protein Delivery

Contact Info

Product

Resources

About