Peptide-based targeting of fluorescent zinc sensors to the plasma membrane of live cells

Abstract

Combining fluorescent zinc sensors with the facile syntheses and biological targeting capabilities of peptides, we created green- and blue-emitting probes that, (i) are readily prepared on the solid-phase, (ii) retain the photophysical and zinc-binding properties of the parent sensor, and (iii) can be directed to the extracellular side of plasma membranes in live cells for detection of mobile zinc.

Fluorescent Sensors for Measuring Metal Ions in Living Systems

“…Recently, Li and co-workers developed ZIMIR, a sensor displayed on the extracellular side of the membrane (Figure 4). 69a The probe consists of fluorescein attached to a DPA Zn 2+ -binding moiety and two dodecyl alkyl chains that anchor it in the plasma membrane. Because it is not membrane permeable, the probe remains anchored to the extracellular side of the cell membrane and was used to detect Zn 2+ release from insulin-secreting cells.…”

Reaction-based fluorescent sensor for investigating mobile Zn 2+ in mitochondria of healthy versus cancerous prostate cells

“…Although protein-based (19) and peptide-based (20) targeting strategies have recently been used to direct the localization of fluorescein-based zinc sensors, controlling the subcellular accumulation of such probes remains a significant challenge.…”

Synthesis, optical properties, laser activity and DFT studies of (E,E)-2,5-bis[2-(1-methyl-1H-pyrrole-2-yl)-vinyl]pyrazine (BMPVP)

“…The photo reactivity of BMPVP has been studied in different solvents namely, CH 2 Cl 2 , CHCl 3 (4) [BMPVP +δ … -δ CHnCl4-n] BMPVP ·+δ Cl -+ · CHnCl3-n contact ion pair (5) The BMPVP dye is highly photostable in polar protic and polar aprotic solvents since no change on the absorbance of the dye occurred upon …”

“…The development of new organic π-conjugated luminophores has been of great interest over the last few decades, due to their wide applications in various fields such as optoelectronics, bio-imaging, and optical storage devices [1][2][3][4]. Organic molecules containing both electron donating (D) and accepting (A) substituents, in a single molecule, exhibit interesting optical and spectral properties due to intramolecular charge transfer (ICT).…”