Peptide-Binding Assessment Using Mass Spectrometry as a New Screening Method for Skin Sensitization

Katô, Hiroshi; Okamoto, Masahiko; Yamashita, Kikuji; Nakamura, Yosuke; Fukumori, Yoshihiro; Nakai, Kiyoshi; Kaneko, Hideo

doi:10.2131/jts.28.19

Cited by 40 publications

(24 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a review of glutathione measurements including peptidomic methods [36] only one group has used human serum as a specimen. KatoOkamoto added compounds to GSH in methanol solution and analyzed them by MALDI -ToF, getting the m/z 308.1 -but it was not done with serum [37].…”

Section: Discussionmentioning

confidence: 99%

Proteomic proof that a probiotic elevates glutathione level in human serum

et al. 2015

View full text Add to dashboard Cite

Lactobacillus fermentum ME-3 (LfME-3) has been proven to synthesize and secrete glutathione. A regular use of the foods fermented by it has shown a favourable influence on human lipid profiles and several antioxidant parameters. We administered the LfME-3-fermented kefir for 14 days to 43 human subjects and evaluated their serum with MALDI-TOF mass spectrometer at the beginning and end of the test period. We found an increase of the peak at m/z 308 (corresponding to glutathione) and a new peak at m/z 1467.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteomic proof that a probiotic elevates glutathione level in human serum

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This depends on the kind of mobile phase (Yilmaz et al 2009). A high pH favors the formation of glutathione conjugated with various compounds (Kato et al 2003) or the oxidation of GSH (Pastore et al 2003). Therefore, it is recommended that various acids be added such as N-ethylmaleimide (NEM), iodoacetic acid (IAA), 2-vinyl pyridine (Pastore et al 2003), trichloroacetic acid (Pastore et al 2003), or trifluoroacetic acid (TFA) (Mitamura et al 2007).…”

Section: Short Review Of a Few Techniques Often Used To Determine Glumentioning

confidence: 99%

“…We tested two matrices: DHB (2,5-dihydroxybenzoic acid) and α-HCCA (α-cyano-4-hydroxycinnamic acid), and different solvents of the analyzed compounds. The HCCA matrix was used by Kato et al (2003) to determine glutathione conjugated with allergenic components. The intensity of the searched conjugates was significantly lower than the HCCA matrix ions (Kato et al 2003).…”

Section: A Short Analysis Of Applicable Ms Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

The Use of Different MS Techniques to Determine Glutathione Levels in Marine Tissues

2012

View full text Add to dashboard Cite

Several techniques were used, mainly mass spectrometry connected with gas chromatography, matrixassisted laser desorption with ionization time-of-flight and mass spectrometry connected with liquid chromatography. A major problem was encountered in determining glutathione, which can be conditioned by the pH and selected reducing agents. The GSH form can be oxidized through derivatization, and a small glutathione amount in the biological samples may hinder the determination process. Another problem is the existence of a metal ion in the tested organism; therefore, often a reagent with a chelating function is added to the sample and the mobile phase in liquid chromatography is applied with appropriate polarity for GSH and GSSG. We determined the concentrations of total, reduced, and oxidized glutathione in the liver, hepatopancreas, muscle, and gonad tissues of brown shrimp (Crangon crangon) and fish (Psetta maxima and Clupea harengus membras). The highest concentrations of tGSH were recorded in the shrimp hepatopancreas (7.21 ± 0.011 μmol g −1 wet weight), in herring liver (2.85±0.025 μmol g −1 ), and in turbot liver (1.86±0.063 μmol g −1). In turn, the highest concentrations were reported for GSSG in the muscle of shrimp (0.140±0.000204 μmol g ). We also investigated seasonal changes in the concentrations of glutathione in the muscle of C. crangon shrimp in the annual cycle. The lowest values of total glutathione were recorded during spring and autumn, which could be correlated with the increase in lipid peroxidation and oxidative stress.

show abstract

“…The local lymph node assay (LLNA) is the most commonly used among several alternatives of skin sensitization tests [1,8,10], including the human cell line activation test (h-CLAT), the peptide-binding test [7], and the quantitative structure-activity relationship (QSAR) system. Furthermore, a three-dimensional human skin model is now being actively developed as an in vitro model for the assessment of primary skin irritation [15], and put into trials for use as an in vitro model for the evaluation of skin sensitization.…”

mentioning

confidence: 99%

Effects of Strain Differences and Vehicles on Results of Local Lymph Node Assays

Anzai

Ullmann²,

Hayashi

et al. 2010

Exp. Anim.

View full text Add to dashboard Cite

Abstract:The Local Lymph Node Assay (LLNA) is now regarded as the worldwide standard. The analysis of accumulated LLNA data reveals that the animal strains and vehicles employed are likely to affect LLNA results. Here we show that an obvious strain difference in the local lymph node response was observed between DMSO-treated CBA/CaOlaHsd and CBA/ CaHsdRcc mice. We also show that a vehicle difference in the response was observed when CBA/CaHsdRcc mice were exposed to 6 vehicles; 4:1 v/v acetone/olive oil (AOO), ethanol/ water (70% EtOH), N,N-dimethylformamide (DMF), 2-butanone (BN), propylene glycol (PG), and dimethylsulfoxide (DMSO). The dpm/LN level was lowest in the 70% EtOH group and highest in the DMSO group. When alpha-hexylcinnamaldehyde (HCA) was used as a sensitizer for the LLNA, HCA was a weak sensitizer when AOO or DMSO was used as a vehicle, but a moderate sensitizer when the other 4 vehicles were used. This study showed that there are vehicle differences in the local lymph node response (dpm/LN level) in the LLNA and that the sensitization potency of HCA may be classified in different categories when using different vehicles. This suggests that careful consideration should be exercised in selecting a vehicle for the LLNA. A further comprehensive study will be needed to investigate why vehicle differences are observed in the LLNA. Key words: animal strain, local lymph node assay, vehicle-effect The local lymph node assay (LLNA) is the most commonly used among several alternatives of skin sensitization tests [1,8,10], including the human cell line activation test (h-CLAT), the peptide-binding test [7], and the quantitative structure-activity relationship (QSAR) system. Furthermore, a three-dimensional human skin model is now being actively developed as an in vitro model for the assessment of primary skin irritation [15], and put into trials for use as an in vitro model for the evaluation of skin sensitization. These newly developed alternative methods are expected to be implemented for practical use in the future. However, a considerable time will be required for these in vitro test methods to become reliable and accepted international standard test methods.In 2001, Takeyoshi et al. developed the non-radioisotope (non-RI) LLNA [12,13] in Japan. The novel test method was expected to become an alternative to the tra--Note-

show abstract

Peptide-Binding Assessment Using Mass Spectrometry as a New Screening Method for Skin Sensitization

Cited by 40 publications

References 16 publications

Proteomic proof that a probiotic elevates glutathione level in human serum

Proteomic proof that a probiotic elevates glutathione level in human serum

The Use of Different MS Techniques to Determine Glutathione Levels in Marine Tissues

Effects of Strain Differences and Vehicles on Results of Local Lymph Node Assays

Contact Info

Product

Resources

About