Peptide-capped functionalized Ag/Au bimetal nanoclusters with enhanced red fluorescence for lysosome-targeted imaging of hypochlorite in living cells

Jia, Minna; Mi, Wenying; Guo, Shaoshi; Yang, Qing‐Zheng; Jin, Yan; Shao, Ning

doi:10.1016/j.talanta.2020.120926

Cited by 45 publications

(27 citation statements)

References 51 publications

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“…Compared with QDs, noble MNCs not only have the advantages of QDs in biological imaging but also have the characteristics of small size, low toxicity, and good biocompatibility. Therefore, they become more attractive fluorescent probes in biological imaging [72,74,75]. Wu et al were the first group to use precious MNCs in near-infrared fluorescence imaging.…”

Section: Noble Mncs In Biotargeting and Bioimagingmentioning

confidence: 99%

Biological nanoscale fluorescent probes: From structure and performance to bioimaging

Wan

Zhang

et al. 2020

Reviews in Analytical Chemistry

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In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.

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Section: Noble Mncs In Biotargeting and Bioimagingmentioning

confidence: 99%

Biological nanoscale fluorescent probes: From structure and performance to bioimaging

Wan

Zhang

et al. 2020

Reviews in Analytical Chemistry

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“…Effort was then put in figuring out the origin of the peak at 460 nm (fluorescence or scatter peak from solvent, water [42,43] or Au NCs). As shown in Figure 2a, water and the aqueous solution of the free oligopeptide show exactly the same emission spectra (λ ex =376 nm), corresponding to the background peak of water [43] .…”

Section: Resultsmentioning

confidence: 99%

Aggregative Growth of Oligopeptide‐Protected Gold Nanoclusters into Plasmonic Particles

Zheng

et al. 2022

ChemNanoMat

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The stimuli response of nanomaterials represents a highly appealing characteristic, whereas the practical applications (in catalysis, biosensing etc.) are largely restricted by the limited understanding on the recognition mechanism. Herein, we report a pH‐regulated size evolution from atomically precise Au nanoclusters (NCs) to plasmonic nanoparticles (NPs). The spectral monitoring demonstrates a three‐stage aggregative growth pathway. First, the non‐covalent interactions among the exterior oligopeptide ligands are gradually enhanced with the increased alkalinity, and then the Au−S bonding is partially disrupted, with the exposed Au atoms aggregating to form relatively larger, polydisperse clusters under weak alkaline conditions. Finally, size‐redistribution occurs slowly under strong alkaline conditions to form the plasmonic particles. The controllable transition sheds light on the transition characteristics from molecular state to metallic state of Au nanomaterials.

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“…While the CCY moiety promoted the formation of Ag/Au alloy NCs, the RGRK-KRRQRRR sequence acted as a cell-penetrating probe through its lysosome-targeting sequence GRKKRRQRRR, and the γ-ECG fragment was able to interact with ROS, improving the stability of NCs in biological environments. Moreover, these fluorescent probes could also be useful for the detection of hypochlorite anion (ClO − ) in living cells, whose presence resulted in fluorescence quenching, redshifted emission maximum, and decreased lifetime [63]. By changing the domain sequence of the capping peptides, it was possible to modulate the binding specificity of metal NCs toward a precise biological target, or cellular organelle.…”

Section: Peptidesmentioning

confidence: 99%

Photoluminescent nanocluster-based probes for bioimaging applications

Bergamaschi

Metrangolo

Dichiarante

2022

Photochem Photobiol Sci

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In the continuous search for versatile and better performing probes for optical bioimaging and biosensing applications, many research efforts have focused on the design and optimization of photoluminescent metal nanoclusters. They consist of a metal core composed by a small number of atoms (diameter < 2-3 nm), usually coated by a shell of stabilizing ligands of different nature, and are characterized by molecule-like quantization of electronic states, resulting in discrete and tunable optical transitions in the UV-Vis and NIR spectral regions. Recent advances in their size-selective synthesis and tailored surface functionalization have allowed the effective combination of nanoclusters and biologically relevant molecules into hybrid platforms, that hold a large potential for bioimaging purposes, as well as for the detection and tracking of specific markers of biological processes or diseases. Here, we will present an overview of the latest combined imaging or sensing nanocluster-based systems reported in the literature, classified according to the different families of coating ligands (namely, peptides, proteins, nucleic acids, and biocompatible polymers), highlighting for each of them the possible applications in the biomedical field.

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Peptide-capped functionalized Ag/Au bimetal nanoclusters with enhanced red fluorescence for lysosome-targeted imaging of hypochlorite in living cells

Cited by 45 publications

References 51 publications

Biological nanoscale fluorescent probes: From structure and performance to bioimaging

Biological nanoscale fluorescent probes: From structure and performance to bioimaging

Aggregative Growth of Oligopeptide‐Protected Gold Nanoclusters into Plasmonic Particles

Photoluminescent nanocluster-based probes for bioimaging applications

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