Peptide cargo administration: current state and applications

Rohira, Harsha; Arora, Arushi; Kaur, Prasanjeet; Chugh, Archana

doi:10.1007/s00253-023-12512-5

Cited by 11 publications

(4 citation statements)

References 197 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, there are more than 60 peptide drugs approved by FDA (Food and Drug Administration), about 150 are in clinical trials and over 500 in pre-clinical studies. [38,39] Considering their low toxicity and the ability to transfer cargo drugs inside the cells, cell penetrating peptides seem to be perfect candidates for preclinical and clinical studies. However, no CPP based therapy has been approved by FDA yet.…”

Section: Discussionmentioning

confidence: 99%

“…Targeting integrins with RGD peptides is an intensively employed strategy for targeted drug delivery, imaging, diagnosis and clinical trials. [39] Here, we conjugated one of the most studied CPP, TAT peptide, with RGD sequence which targets integrin receptors. Then, we analysed the selectivity of the new peptide for integrin and its penetrating properties in model membranes of the new peptide.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Integrin Facilitates the Internalization of TAT Peptide Conjugated to RGD Motif in Model Lipid Membranes

Ciobanasu,

Pernier,

Le Clainche

2023

ChemBioChem

View full text Add to dashboard Cite

In recent years, targeted drug delivery has attracted a great interest for enhanced therapeutic efficiency, with diminished side effects, especially in cancer therapy. Cell penetrating peptides like HIV1‐TAT peptide, appear to be the perfect vectors for translocating drugs or other cargoes across the plasma membrane, but their application is limited mostly due to insufficient specificity for intended targets. The tripeptide motif RGD (arginine‐glycine‐aspartate), found in extracellular matrix proteins has high affinity for integrin receptors overexpressed in cancer and it is involved in different phases of disease progression, including proliferation, invasion and migration. Discovery of new peptides with high binding affinity for disease receptors and permeability of plasma membranes is desirable for both, development of targeted drug delivery systems and early detection and diagnosis. To complement the TAT peptide with specific targeting ability, we conjugated it with an integrin‐binding RGD motif. In this paper we describe the permeability abilities and specificity for integrin receptor of RGD‐TAT peptides in model membranes. Our findings reveal that RGD‐TAT peptide maintains its ability to permeate lipid membranes and exhibits specificity for integrin receptors embedded in giant unilamellar vesicles. This promising outcome suggests that the peptide has significant potential for aplications in targeted drug delivery systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrin Facilitates the Internalization of TAT Peptide Conjugated to RGD Motif in Model Lipid Membranes

Ciobanasu,

Pernier,

Le Clainche

2023

ChemBioChem

View full text Add to dashboard Cite

show abstract

“…This has led to the emergence of various delivery techniques such as nanoparticles, cell-penetrating peptides, and antibody-drug conjugates. However, the effectiveness of these methods is limited by (1) poor cellular uptake, (2) lack of specificity for antigen-presenting cells (APCs), and (3) inability to escape from intracellular compartments. [1][2][3][4] When particles and proteins are taken up by cells, they are trafficked from the early and late endosomes to the lysosomes, where they are degraded.…”

Section: Introductionmentioning

confidence: 99%

“…However, the effectiveness of these methods is limited by (1) poor cellular uptake, (2) lack of specificity for antigen-presenting cells (APCs), and (3) inability to escape from intracellular compartments. [1][2][3][4] When particles and proteins are taken up by cells, they are trafficked from the early and late endosomes to the lysosomes, where they are degraded. [4][5][6] The use of bacteria changes the traditional notion of "delivery."…”

Section: Introductionmentioning

confidence: 99%

A Bacterial mRNA‐Lysis‐Mediated Cargo Release Vaccine System for Regulated Cytosolic Surveillance and Optimized Antigen Delivery

Li,

Sun,

Zhang

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Engineered vector‐based in vivo protein delivery platforms have made significant progress for both prophylactic and therapeutic applications. However, the lack of effective release strategies results in foreign cargo being trapped within the vector, restricting the provision of significant performance benefits and enhanced therapeutic results compared to traditional vaccines. Herein, the development of a Salmonella mRNA interferase regulation vector (SIRV) system is reported to overcome this challenge. The genetic circuits are engineered that (1) induce self‐lysis to release foreign antigens into target cells and (2) activate the cytosolic surveillance cGAS‐STING axis by releasing DNA into the cytoplasm. Delayed synthesis of the MazF interferase regulates differential mRNA cleavage, resulting in a 36‐fold increase in the delivery of foreign antigens and modest activation of the inflammasome, which collectively contribute to the marked maturation of antigen‐presenting cells (APCs). Bacteria delivering the protective antigen SaoA exhibits excellent immunogenicity and safety in mouse and pig models, significantly improving the survival rate of animals challenged with multiple serotypes of Streptococcus suis. Thus, the SIRV system enables the effective integration of various modular components and antigen cargos, allowing for the generation of an extensive range of intracellular protein delivery systems using multiple bacterial species in a highly efficient manner.

show abstract

Advanced Targeting Systems for Tissue-Specific Drug Delivery

Nastasi

2024

Interdisciplinary Cancer Research

View full text Add to dashboard Cite

Peptide cargo administration: current state and applications

Cited by 11 publications

References 197 publications

Integrin Facilitates the Internalization of TAT Peptide Conjugated to RGD Motif in Model Lipid Membranes

Integrin Facilitates the Internalization of TAT Peptide Conjugated to RGD Motif in Model Lipid Membranes

A Bacterial mRNA‐Lysis‐Mediated Cargo Release Vaccine System for Regulated Cytosolic Surveillance and Optimized Antigen Delivery

Advanced Targeting Systems for Tissue-Specific Drug Delivery

Contact Info

Product

Resources

About