Peptide-Conjugated Silver Nanoparticle for Autoantibody Recognition

Higa, Akemi Martins; Mambrini, G. P.; Ierich, Jéssica Cristiane Magalhães; García, Pamela Soto; Scramin, Juliana A; Peroni, L; Okuda-Shinagawa, Nancy M.; Miranda, Maria Terêsa Machini de; Trivinho‐Strixino, Francisco; Leite, Fábio L.

doi:10.1166/jnn.2019.16734

Cited by 8 publications

(3 citation statements)

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“…Beyond DNA, there are many other possibilities to functionalize silver nanoparticles with biomolecules, such as using antibiotics [91][92][93], amines [94,95], chitosan [96], polymeric materials [97,98], glycolipids [99], amino acids [100,101] and peptides [102,103]. For example, Aboelmaati and co-workers loaded green-synthesized AgNPs with two antibiotics, moxifloxacin and gatifloxacin, for antibacterial activity against bacterial biofilms [104].…”

Section: Silver Nanoparticles Functionalized By Biomoleculesmentioning

confidence: 99%

Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Menichetti

Mavridi-Printezi

Mordini

et al. 2023

JFB

152

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Silver nanoparticles (AgNPs) are the most investigated antibacterial agents against multidrug resistant (MDR) pathogens. They can lead to cellular death by means of different mechanisms, damaging several cell compartments, from the external membrane, to enzymes, DNA and proteins; this simultaneous attack amplifies the toxic effect on bacteria with respect to traditional antibiotics. The effectiveness of AgNPs against MDR bacteria is strongly correlated with their chemical and morphological properties, which influence the pathways involved in cellular damage. In this review, AgNPs’ size, shape and modification by functional groups or other materials are reported, both to investigate the different synthetic pathways correlated with nanoparticles’ modifications and to evaluate the related effect on their antibacterial activity. Indeed, understanding the synthetic conditions for obtaining performing antibacterial AgNPs could help to tailor new and improved silver-based agents to combat multidrug resistance.

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Section: Silver Nanoparticles Functionalized By Biomoleculesmentioning

confidence: 99%

Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Menichetti

Mavridi-Printezi

Mordini

et al. 2023

JFB

152

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“…To address these challenges, the use of nanoparticles as a multimeric presentation system is an alternative approach, which should provide high sensitivity and solve the epitope–adhesion problem [6,9,51,52]. We have previously shown that functionalized nanoparticles derived from TuMV significantly increased antibody detection with respect to conventional systems [16,17,46].…”

Section: Discussionmentioning

confidence: 99%

Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection

et al. 2019

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Nanoparticles derived from the elongated flexuous capsids of Turnip mosaic virus (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs.

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“…Nanomaterials have been widely used in pharmaceutical, agricultural, industrial, and household products (Higa et al, ; Shokri, Isapour, Hosseini, & Zarbpoor, ; Wu & Kuo, ). More than 50% of nanomaterials used in industry contain silver nanoparticles (AgNPs), and it is estimated that 12.2–1,216 tons of AgNPs would be produced worldwide in 2020 (Nowack, Krug, & Height, ).…”

Section: Introductionmentioning

confidence: 99%

Aggregation kinetics and mechanisms of silver nanoparticles in simulated pollution water under UV light irradiation

Zhang

Shang

et al. 2019

Water Environment Research

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This paper investigated the effect mechanism of complex components (fulvic acid [FA], sodium dodecylbenzene sulfonate [SDBS], and sodium nitrate [NaNO 3 ]) on the aggregation kinetics of polyvinylpyrrolidone-modified silver nanoparticles (PVP-AgNPs) under UV irradiation. The results showed that FA and NaNO 3 alone did not cause aggregation due to the high steric hindrance and/or electrostatic repulsive forces. In high concentration of SDBS solution (20-50 mM), the stability of PVP-AgNPs was reduced by adsorbing SDBS on nanoparticle surface and replacing their PVP coatings. A mixed system of two pollutants had a synergistic effect on PVP-AgNPs aggregation. In the mixed system of SDBS and FA, the interaction of SDBS and PVP-AgNPs dominated the aggregation of PVP-AgNPs. NaNO 3 significantly improved the aggregation rate of PVP-AgNPs in SDBS solution due to the charge neutralization effect of electrolyte. In 20 mg/L FA solution, the aggregation rate increased slightly with increasing NaNO 3 concentration from 50 to 200 mM due to the charge neutralization effect, while the hydrodynamic diameters of PVP-AgNPs increased linearly and rapidly to micrometer size because the spatial conformation of adsorbed FA became compact in high-salinity solution. The calculation results of eDLVO theory were basically consistent with most of the experimental results. © 2019 Water Environment Federation • Practitioner points• PVP-AgNPs was uniformly dispersed in NaNO 3 or FA solution under UV irradiation. • PVP-AgNPs formed aggregates in SDBS solutions under UV irradiation.• A system with two mixed pollutants had a synergistic effect on promoting aggregation of PVP-AgNPs. • eDLVO theory could explain the aggregation results in different chemical conditions except in NaNO 3 solution.

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Peptide-Conjugated Silver Nanoparticle for Autoantibody Recognition

Cited by 8 publications

References 0 publications

Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection

Aggregation kinetics and mechanisms of silver nanoparticles in simulated pollution water under UV light irradiation

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