Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

Wang, Zheng; Guo, Yingchang; Zhang, Guangtan; Bai, Junwei; Song, Yucheng; Song, Xiaofei; Zhu, Qinhui; Bao, Xuebin; Wu, Gang; Zhang, Chao

doi:10.1371/journal.pone.0287133

Cited by 12 publications

(4 citation statements)

References 41 publications

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“…Mechanistically, micropeptides are able to contribute to the tumor phenotype by affecting the tumor microenvironment like blood vessels and extracellular matrix (ECM) 91 . For example, lncRNA BVES‐AS1 was demonstrated to encode a 50‐aa‐length micropeptide termed BVES‐AS1‐201‐50aa.This micropeptide increases the expression of PCNA and MMP9 in the tumor microenvironment, thus promoting CRC cells viability, migration, and invasion 92 . However, whether one micropeptide acts via several mechanisms to affect the phenotypes of the same tumor or whether one micropeptide plays diverse roles in different tumors via more subtle and specific mechanisms should be explored further.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Subcellular localization and relevant mechanisms of human cancer‐related micropeptides

Deng,

Xu,

Jie

et al. 2023

The FASEB Journal

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Rapid advances in high‐quality sequencing and bioinformatics have invalidated the argument that noncoding RNAs (ncRNAs) are junk transcripts that do not encode proteins. Increasing evidence suggests that small open reading frames (sORFs) in ncRNAs can encode micropeptides and polypeptides within 100 amino acids in length. Several micropeptides have been characterized and proven to have various functions in human physiology and pathology, particularly in cancer. The present review mainly highlights the latest studies on ncRNA‐encoded micropeptides in different cancers and categorizes them based on their subcellular localization, thereby providing a theoretical basis for micropeptide applications in the early diagnosis and prognosis of cancer and as therapeutic targets. However, considering the inherent characteristics of micropeptides and the limitations of the assay technology methods, more detailed information is warranted.

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Section: Conclusion and Discussionmentioning

confidence: 99%

Subcellular localization and relevant mechanisms of human cancer‐related micropeptides

Deng,

Xu,

Jie

et al. 2023

The FASEB Journal

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“…Co-IP experiment was done as described (Zheng et al 2023 ) using 10 7 LINC00954-ORF construct-transfected A549 cells. Briefly, proteins extracted from the cells were immunoprecipitated and purified with anti-Flag tag affinity Sepharose gels or anti-IgG controls (Yeasen, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells

Han,

Chen,

Sun

et al. 2024

Amino Acids

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The advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). In the present study, bioinformatics was used to screen the lncRNA-hidden polypeptides in LUAD. Analysis of protein expression was done by western blot or immunofluorescence assay. The functions of the polypeptide were determined by detecting its effects on cell viability, proliferation, migration, invasion, and pemetrexed (PEM) sensitivity. The protein interactors of the polypeptide were analyzed by mass spectrometry after Co-immunoprecipitation (Co-IP) assay. The results showed that the lncRNA LINC00954 was confirmed to encode a novel polypeptide LINC00954-ORF. The polypeptide had tumor-suppressor features in A549 cells by repressing cell growth, motility and invasion. Moreover, the polypeptide enhanced PEM sensitivity and suppressed growth in A549/PEM cells. The protein interactors of this polypeptide had close correlations with RNA processing, amide metabolic process, translation, RNA binding, RNA transport, and DNA replication. As a conclusion, the LINC00954-ORF polypeptide embedded in lncRNA LINC00954 possesses tumor-suppressor features in A549 and PEM-resistant A549 cells and sensitizes PEM-resistant A549 cells to PEM, providing evidence that the LINC00954-ORF polypeptide is a potential anti-cancer agent in LUAD.

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“…Knockdown of BBOX1-AS1 enhances cancer cell migration and invasion, with its regulatory axis being hsa-miR-361-3p/SH2B1 ( 75 ). BVES-AS1 can encode a micropeptide that promotes the migration and invasion of CRC cells in vitro ( 77 ). Zhang et al.…”

Section: Lncrnas Regulate Invasion and Metastasismentioning

confidence: 99%

The functions and mechanisms of long non-coding RNA in colorectal cancer

Lin,

Zhao,

et al. 2024

Front. Oncol.

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CRC poses a significant challenge in the global health domain, with a high number of deaths attributed to this disease annually. If CRC is detected only in its advanced stages, the difficulty of treatment increases significantly. Therefore, biomarkers for the early detection of CRC play a crucial role in improving patient outcomes and increasing survival rates. The development of a reliable biomarker for early detection of CRC is particularly important for timely diagnosis and treatment. However, current methods for CRC detection, such as endoscopic examination, blood, and stool tests, have certain limitations and often only detect cases in the late stages. To overcome these constraints, researchers have turned their attention to molecular biomarkers, which are considered a promising approach to improving CRC detection. Non-invasive methods using biomarkers such as mRNA, circulating cell-free DNA, microRNA, LncRNA, and proteins can provide more reliable diagnostic information. These biomarkers can be found in blood, tissue, stool, and volatile organic compounds. Identifying molecular biomarkers with high sensitivity and specificity for the early and safe, economic, and easily measurable detection of CRC remains a significant challenge for researchers.

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Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

Cited by 12 publications

References 41 publications

Subcellular localization and relevant mechanisms of human cancer‐related micropeptides

Subcellular localization and relevant mechanisms of human cancer‐related micropeptides

A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells

The functions and mechanisms of long non-coding RNA in colorectal cancer

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