Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media

Farges-Haddani, B.; Tessier, B.; Chenu, S.; Chevalot, Isabelle; Harscoat, C.; Marc, Ivan; Goergen, Jean‐Louis; Marc, Annie

doi:10.1016/j.procbio.2006.06.002

Cited by 53 publications

(31 citation statements)

References 38 publications

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“…4a, b). Hence, the variations of the cell specific growth rate observed when hydrolysates were added, did not result from cell death prevention nor from anti-apoptotic effects, contrarily to the hypothesis proposed by other authors (Schlaeger 1996;Farges-Haddani et al 2006;Franek and Katinger 2002;Franek 2004).…”

Section: Kinetics Of Cho Cell Growth and Deathsupporting

confidence: 55%

“…During the first 45 h of culture, a high yield of lactate produced per glucose consumed (Y Lac/Glc ) of 1.8 mol mol -1 was measured ( Fig. 5b; Table 1) compared to values from 1.2 to 1.6 mol mol -1 commonly reported (Lao and Toth 1997;Farges-Haddani et al 2006;Sung et al 2004;Ahn and Antoniewicz 2011). Consequently, about 90 % of the glucose flow was directed toward lactate production instead of integrating the TCA cycle.…”

Section: Glycolysismentioning

confidence: 73%

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Combination of yeast hydrolysates to improve CHO cell growth and IgG production

et al. 2012

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Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L -1 increased the maximal cell density by 70 %, while a mixture of YE (1 g L -1 ) and YP.A (4 g L -1 ) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.

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Section: Kinetics Of Cho Cell Growth and Deathsupporting

confidence: 55%

Section: Glycolysismentioning

confidence: 73%

Combination of yeast hydrolysates to improve CHO cell growth and IgG production

et al. 2012

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“…Farges-Haddani et al 96 reported that rapeseed protein hydrolysates of a specifi c peptide composition could be used as substitutes for bovine serum in animal cell culture media.…”

Section: 95mentioning

confidence: 99%

The biochemurgist –Bioconversion of agricultural raw materials for chemical production

Koutinas

Wang

Webb

2007

Biofuels Bioprod Bioref

110

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Current cereal-and oilseed-based processes for the production of chemicals should be restructured into advanced biorefi ning strategies in order to reduce environmental impact, improve overall economics and meet market/societal needs. Next generation biorefi neries should utilize all grain or oilseed components as well as any associated residues for the production of a spectrum of products ranging from biofuels and biodegradable plastics to commodity, platform and speciality chemicals. Generic biorefi ning concepts are proposed in the case of wheat and rapeseed. Glycerol produced as by-product from biodiesel production could be upgraded into an important raw material for the production of various chemicals through microbial bioconversions or enzymatic biotransformations.

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“…25 These peptones are composed of complex mixtures containing amino acids, oligopeptides, and other substances such as phenolic compounds and fibres. 8 Transferrin is a common ingredient of serum-free media; it is functional for transport of iron across the plasma membrane. To replace transferrin from serum-free formulations, it is possible to use an iron salt combined to an iron chelator such as Fe 3þ -sulfate-iminodiacetic acid (IDA) complexes or Fe 3þ -sulfate-glycylglycine (gly-gly) complexes.…”

Section: Discussionmentioning

confidence: 99%

“…Culture media such as MEM (Eagle's Minimum Essential Medium), DMEM (Dulbecco's Modified Eagle's Medium) and M199 are used for the cultivation of adherent cells to produce viral suspensions. 8,9 Because cell culture systems were established in vitro, serum has been used most commonly. The presence of serum in the medium allows to mimic the in vivo cell environment and to stimulate their growth.…”

Section: Introductionmentioning

confidence: 99%

Development of an animal‐component free medium for vero cells culture

Rourou

Ark²,

Velden³

et al. 2009

Biotechnology Progress

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This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that affect cell growth. Using Medium 199 or MEM as a basal medium, a serum-free medium (SFM) referred as IPT-SFM that only enclosed transferrin as a component of animal origin was developed at first. Then, the composition of IPT-SFM was further improved to obtain an animal-component free medium named IPT-AFM. IPT-AFM contains M199 as a basal medium, plant hydrolysates, epidermal growth factor, ethanolamine, ferric citrate, and vitamin C. Among various plant hydrolysates, specific combinations of soy (Hypep 1510) and wheat gluten (Hypeps 4601 and 4605) hydrolysates, were identified to promote cell growth; whereas individual Hypeps had a minor positive effect on cell growth. Nevertheless, the removal of serum did influence cell attachment. Coating tissue-culture flasks with teleostean, a product extracted from cold water fish skin, had not only enhanced cell attachment but also improved cell growth performance in static cultures. Different non-animal proteases were also assessed as an alternative to trypsin. TrypLE Select, a recombinant trypsin, gave the best cell growth performances. Kinetics of cell growth in IPT-AFM were investigated in T-flasks, cell growth was comparable with that obtained in MEM+10% fetal calf serum (FCS). A mean cell division number equal to 2.26 +/- 0.18 and a specific growth rate micro 0.019 +/- 0.003 h(-1) were achieved in IPT-AFM.

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Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media

Cited by 53 publications

References 38 publications

Combination of yeast hydrolysates to improve CHO cell growth and IgG production

Combination of yeast hydrolysates to improve CHO cell growth and IgG production

The biochemurgist –Bioconversion of agricultural raw materials for chemical production

Development of an animal‐component free medium for vero cells culture

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