Peptide Isolated from Noni Seeds Confers Gastroprotective Effect by Improving Inflammation and Oxidative Stress in Mice

Abstract

Plant molecules are continuously investigated to prevent and treat in ammatory and ulcerative disorders associated with the gastrointestinal tract, such as gastritis, colitis, mucositis, and ulcer. However, most of the work published is devoted to investigating the therapeutic properties of secondary plant metabolites. This work investigated the gastroprotective activity of a lipid transfer protein isolated from Morinda citrifolia L., named McLTP 1 , when orally administered to mice, from the perspective of its use as a novel peptide-based drug for the prevention and treatment of ulcerative gastric lesions. Pretreatment with McLTP 1 at different doses (4, 8, or 16 mg/kg) reduced ethanol-induced gastric lesions (p < 0.05) in 40%, 84%, and 88%, respectively. In ethanol-induced gastric lesions, it was demonstrated alterations in levels of GSH (↑100%; p < 0.05) and a reduction by 45% in the levels of MDA (p < 0.05) after McLTP 1 administration (8 mg/kg). McLTP 1 showed an anti-in ammatory effect through the modulation of the cytokines IL-10 (↑33%) and TNF-α (↓54%) and was able to reduce MPO levels (↓95%) in the gastric tissue. Besides, the gastroprotective of McLTP 1 also involves the production of nitric oxide. The present ndings reveal that McLTP 1 has a gastroprotective effect dependent, at least in part, on its antiin ammatory and antioxidant effects.

McLTP1, a lipid transfer protein isolated from noni seeds induces effective healing of superficial burns

“…Thermal injury increases MDA levels which were reduced by both concentrations of McLTP1. This result is in accordance with other protocols 32 . Metabolization of nitric oxide (NO) in NO2 and NO3 also contributes to increase oxidative stress and tissue damage 59 .…”

“…Only McLTP1 increased GSH levels after burn, showing its potential to reverse injuries caused by superficial burns. Similar results were observed previously 32 .…”

“…Levels of TNF-a, IL-1b and IL-6 were reduced while IL-10 was increased by highest concentration of McLTP1. The modulation of cytokines was observed previously, in pep ulcer model 32 .…”

“…Levels of TNF-α, IL-1β and IL-6 were reduced while IL-10 was increased by highest concentration of McLTP1. The modulation of cytokines was observed previously, in pep ulcer model 32 . Immunohistochemistry of TNF-α showed increased of immunostaining after 3 days, proving the importance of this mediator in early stages of burn injury 42–44 and corroborating with our previous results.…”

“…Mc LTP1 is a lipid transfer protein isolated from Noni seeds that has been demonstrating anti-inflammatory, antinociceptive and antioxidant effects 29,30,31,32 . Recently, this protein showed an in vitro antibacterial effect in Sthaphylococcus aureus and Staphylococcus epidermidis 33 , predominant bacteria in secondary skin infections 34,35 .…”