Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells

Abstract

Background:The neurotrophic receptor tyrosine kinase B (TrkB) has diverse signaling roles in neurons and tumor cells. Accordingly, its suppressive targeting is of interest in neuroblastoma and other tumors, whereas its role in improving survival is focused in neurons. Here we describe targeting of TrkB-binding peptide-conjugated liposomes (PCL) to the TrkB-expressing mouse macrophage-like cell line RAW264, and to all-trans-retinoic acid-treated neuron-like TrkB Conclusion:We demonstrate here the feasibility of targeting liposomes to TrkB-expressing cells by 18-mer peptides, promoting cellular uptake (at least partly into endosomes) via receptormediated pathways.

Peptide selected by phage display increases survival of SH‐SY5Y neurons comparable to brain‐derived neurotrophic factor

“…NTRK2 and p75NTR cDNAs were amplified by primer pairs of Ranjan and Do, respectively. 32,33 The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene was used as an endogenous control. Finally, relative changes in gene expression levels were calculated by the threshold (quantification) cycle.…”

Nanoparticle mediated drug delivery of rolipram to tyrosine kinase B positive cells in the inner ear with targeting peptides and agonistic antibodies

“…Polymerosomes, lipid core nanocapsules (LNCs), silica as well as quantum dot NPs (QDotNPs) were used for the presented study. Peptide ligands are summarized in Figure 5 and described previously (Ranjan et al, 2012 ). 29D7 monoclonal anti-TrkB-antibody was kindly provided by Wyeth Research, Pfizer®, Connecticut, USA and characterized previously (Steketee et al, 2011 ).…”

“…The neuroblastoma cell line SH-SY5Y was differentiated by all-trans-retinoic acid (ATRA) treatment that induces expression of TrkB, but not of TrkA receptor, and mediates biological responsiveness to receptors for the neurotrophins BDNF and NT-4/5 as described previously by our group (Ranjan et al, 2012 ). As an alternative to SH-SY5Y cells, stable TrkB-transfected G7 cells were received from Garrett Brodeur, M.D.…”

“…SHSY5Y cells were grown in DMEM/F12 1:1 medium (PAA, Linz, Austria) supplemented with 2 mM L-glutamine, penicillin (20 units/ml), streptomycin (20 mg/ml), and 15% (v/v) heat-inactivated fetal calf serum (PAA®) (Ranjan et al, 2012 ). Cells were maintained at 37°C in a saturated humidity atmosphere containing 95% air and 5% CO 2 .…”

“…Typically these peptides are identified via the screening tool phage display (Kehoe and Kay, 2005 ; Deutscher, 2010 ), allowing selection of peptide sequences with increased affinities to a specific target of choice displayed on bacteriophage capsids that are collected and amplified with infected E. coli . Numerous sequences have been tested (Friedman et al, 2013 ), we designed peptides based on Ma et al ( 2003 ) and summarized here (Ranjan et al, 2012 ).…”