Peptide microarray-based fluorescence assay for quantitatively monitoring the tumor-associated matrix metalloproteinase-2 activity

Jian, Minghong; Su, Min; Gao, Jiaxue

doi:10.1016/j.snb.2019.127320

Cited by 15 publications

(13 citation statements)

References 40 publications

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“…Because of their inherent antibiofouling nature, the fabrication of microarrays on the polymer brush modified substrates allows biomolecular immobilization and recognition with low nonspecific interactions, resulting in a significant improvement of the specificity and reproducibility of microarray [77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95]. In addition, the 3D structure of polymer brush modified substrates provide a high biomolecule immobilization capacity and accessible scaffolds with sufficient space for biomolecule binding, leading to an increase in the sensitivity of microarrays.…”

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

“…This finding opens a new era in glycobiology, where the detection of glycan-binding proteins (e.g., lectins) meets the requirements of medical and biological events. The analytical performance of microarray can be further improved by integr advantages of nanomaterials and polymer brushes [91][92][93][94][95][96]. Liu et al developed a polymer brush hierarchical nanostructure-modified glass slide (term PGMA@3D(160) substrate) for fabricating high-performance microarrays throug ing a poly(glycidyl methacrylate) (PGMA) brush layer on the 160 nm silica part assembled monolayer coated glass slide [92].…”

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

“…As a ty ample, the interactions of three monosaccharides (4-aminophenyl β-D-galactopyr 4-aminophenyl β-D-glucopyranoside, and 4-aminophenyl α-D-mannopyranosi two lectins (biotinylated ricinus communis agglutinin 120 and biotinylated conc A from Canavalia ensiformis) have been assessed by PGMA@3D(160) substrate-b bohydrate microarrays. The carbohydrate microarrays exhibited good selectivit multivalent interaction and low LOD in the picomolar range without any signal cation, making it a promising platform for bioanalytical and biomedical app The analytical performance of microarray can be further improved by integrating the advantages of nanomaterials and polymer brushes [91][92][93][94][95][96]. Liu et al developed a spherepolymer brush hierarchical nanostructure-modified glass slide (termed as PGMA@3D(160) substrate) for fabricating high-performance microarrays through growing a poly(glycidyl methacrylate) (PGMA) brush layer on the 160 nm silica particle-self-assembled monolayer coated glass slide [92].…”

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

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The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

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Polymers with different structures and morphology have been extensively used to construct functionalized surfaces for a wide range of applications because the physicochemical properties of polymers can be finely adjusted by their molecular weights, polydispersity and configurations, as well as the chemical structures and natures of monomers. In particular, the specific functions of polymers can be easily achieved at post-synthesis by the attachment of different kinds of active molecules such as recognition ligand, peptides, aptamers and antibodies. In this review, the recent advances in the bioanalytical and biomedical applications of polymer modified substrates were summarized with subsections on functionalization using branched polymers, polymer brushes and polymer hydrogels. The review focuses on their applications as biosensors with excellent analytical performance and/or as nonfouling surfaces with efficient antibacterial activity. Finally, we discuss the perspectives and future directions of polymer modified substrates in the development of biodevices for the diagnosis, treatment and prevention of diseases.

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Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

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The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

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“…The results found in this approach have constantly been expanding. They are promising and are increasingly being used in the biomedical area for the development/identification of new biomarkers, as well as for screening antigens, delivery systems and drug discovery and are mainly used in viral diagnosis and cancer research [ 174 , 175 ].…”

Section: Approaches Used To Search For New Diagnostic Candidatesmentioning

confidence: 99%

Challenges in Serologic Diagnostics of Neglected Human Systemic Mycoses: An Overview on Characterization of New Targets

et al. 2022

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Systemic mycoses have been viewed as neglected diseases and they are responsible for deaths and disabilities around the world. Rapid, low-cost, simple, highly-specific and sensitive diagnostic tests are critical components of patient care, disease control and active surveillance. However, the diagnosis of fungal infections represents a great challenge because of the decline in the expertise needed for identifying fungi, and a reduced number of instruments and assays specific to fungal identification. Unfortunately, time of diagnosis is one of the most important risk factors for mortality rates from many of the systemic mycoses. In addition, phenotypic and biochemical identification methods are often time-consuming, which has created an increasing demand for new methods of fungal identification. In this review, we discuss the current context of the diagnosis of the main systemic mycoses and propose alternative approaches for the identification of new targets for fungal pathogens, which can help in the development of new diagnostic tests.

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“…Compared to fluorescence, OIRD shows much lower sensitivity and generally several orders of magnitude higher limit of detection (LOD) for protein microarrays. 4,5,8 Its sensitivity is even inferior to the angle-scanning based SPR technique. 29–31 It is worth noting, however, that in previous work OIRD has shown astonishing sensitivity to probe sub-monolayer (0.05 monolayer) gas adsorption/desorption and 0.01 monolayer epitaxial layer growth of oxide films.…”

Section: Introductionmentioning

confidence: 99%