Peptide signaling molecules <scp>CLE</scp>5 and <scp>CLE</scp>6 affect Arabidopsis leaf shape downstream of leaf patterning transcription factors and auxin

DiGennaro, Peter; Grienenberger, Etienne; Dao, Thai Q.; Jun, Ji Hyung; Fletcher, Jennifer

doi:10.1002/pld3.103

Cited by 21 publications

(14 citation statements)

References 61 publications

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“…We generated an hct null mutant by CRISPR/Cas9-mediated gene inactivation as described earlier (DiGennaro et al, 2018). Briefly, BbsI restriction enzyme was used to introduce into the At-psgR/GW plasmid a double strand fragment resulting from 5’-GATTGCTCGGTGGCAGGCCGGACCA and 5’-AAACTGGTCCGGCCTGCCACCGAGC oligonucleotides annealing, which targets HCT region CTCGGTGGCAGGCCGGACCATGG.…”

Section: Methodsmentioning

confidence: 99%

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Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Kriegshauser

Knosp

Grienenberger

et al. 2020

Preprint

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The plant phenylpropanoid pathway generates a major class of specialized metabolites and precursors of essential extracellular polymers that initially appeared upon plant terrestrialization. Despite its evolutionary significance, little is known about the complexity and function of this major metabolic pathway in extant bryophytes, the ancestors of which were the first land plants. Here, we report that the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE (HCT) gene, which plays a critical function in the phenylpropanoid pathway during seed plant development, is functionally conserved in Physcomitrium (Physcomitrella) patens, in the moss lineage of bryophytes. Phylogenetic analysis indicates that bona fide HCT function emerged in the progenitor of embryophytes. In vitro enzyme assays, moss phenolic pathway reconstitution in yeast and in planta gene inactivation coupled to targeted metabolic profiling collectively indicate that P. patens HCT (PpHCT), similar to tracheophyte HCT orthologs, uses shikimate as a native acyl acceptor to produce a p-coumaroyl-5-O-shikimate intermediate. Phenotypic and metabolic analyses of loss-of-function mutants show that PpHCT is necessary for the production of caffeate derivatives, including previously reported caffeoyl-threonate esters, and for the formation of an intact cuticle. Deep conservation of HCT function in embryophytes is further suggested by the ability of HCT genes from P. patens and the liverwort Marchantia polymorpha to complement an Arabidopsis thaliana CRISPR/Cas9 hct mutant, and by the presence of phenolic esters of shikimate in representative species of the three major bryophyte lineages.One sentence summaryMultidisciplinary study of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE (HCT) gene in Physcomitrium (Physcomitrella) patens points to its functional conservation in embryophytes.

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Section: Methodsmentioning

confidence: 99%

“…DiGennaro et al, 2018). Briefly, BbsI restriction enzyme was used to introduce into the Atwhich targets HCT region CTCGGTGGCAGGCCGGACCATGG.…”

mentioning

confidence: 99%

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Kriegshauser

Knosp

Grienenberger

et al. 2020

Preprint

Self Cite

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“…BOP1/2 promote the expression of FIL , which could stimulate the mediolateral expansion of the leaf blade at specific positions along the proximodistal axis [134]. In addition, BOP1/2 promote the expression of two proximally expressed CLE (CLAVATA-LIKE EMBRYO SURROUNDING REGION) peptide-encoding genes that are simultaneously upregulated by WOX1 and repressed by AS2 [137]. CLEs typically have intercellular signaling roles and bind to leucine-rich receptor-like kinases.…”

Section: Control Of Mediolateral Patterning Along the Proximodistamentioning

confidence: 99%

Coordination of Leaf Development Across Developmental Axes

Satterlee

Scanlon

2019

Plants

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Leaves are initiated as lateral outgrowths from shoot apical meristems throughout the vegetative life of the plant. To achieve proper developmental patterning, cell-type specification and growth must occur in an organized fashion along the proximodistal (base-to-tip), mediolateral (central-to-edge), and adaxial–abaxial (top-bottom) axes of the developing leaf. Early studies of mutants with defects in patterning along multiple leaf axes suggested that patterning must be coordinated across developmental axes. Decades later, we now recognize that a highly complex and interconnected transcriptional network of patterning genes and hormones underlies leaf development. Here, we review the molecular genetic mechanisms by which leaf development is coordinated across leaf axes. Such coordination likely plays an important role in ensuring the reproducible phenotypic outcomes of leaf morphogenesis.

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“…Previous studies have indicated that the CLE5 and CLE6 peptides singly and together showed the rosette leaf shape phenotype. These two genes are also expressed at the base above the abscission zone of the flower organ (Jun et al, 2010;DiGennaro et al, 2018). GhCLE28.1 was found to be in the same subfamily as AtCLE5 and AtCLE6.…”

Section: Discussionmentioning

confidence: 99%

Molecular Traits and Functional Analysis of the CLAVATA3/Endosperm Surrounding Region-Related Small Signaling Peptides in Three Species of Gossypium Genus

et al. 2021

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The CLAVATA3/endosperm surrounding region-related (CLE) small peptides are a group of C-terminally encoded and post-translationally modified signal molecules involved in regulating the growth and development of various plants. However, the function and evolution of these peptides have so far remained elusive in cotton. In this study, 55, 56, and 86 CLE genes were identified in the Gossypium raimondii, Gossypium arboreum, and Gossypium hirsutum genomes, respectively, and all members were divided into seven groups. These groups were distinctly different in their protein characteristics, gene structures, conserved motifs, and multiple sequence alignment. Whole genome or segmental duplications played a significant role in the expansion of the CLE family in cotton, and experienced purifying selection during the long evolutionary process in cotton. Cis-acting regulatory elements and transcript profiling revealed that the CLE genes of cotton exist in different tissues, developmental stages, and respond to abiotic stresses. Protein properties, structure prediction, protein interaction network prediction of GhCLE2, GhCLE33.2, and GhCLE28.1 peptides were, respectively, analyzed. In addition, the overexpression of GhCLE2, GhCLE33.2, or GhCLE28.1 in Arabidopsis, respectively, resulted in a distinctive shrub-like dwarf plant, slightly purple leaves, large rosettes with large malformed leaves, and lack of reproductive growth. This study provides important insights into the evolution of cotton CLEs and delineates the functional conservatism and divergence of CLE genes in the growth and development of cotton.

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Peptide signaling molecules CLE5 and CLE6 affect Arabidopsis leaf shape downstream of leaf patterning transcription factors and auxin

Cited by 21 publications

References 61 publications

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Coordination of Leaf Development Across Developmental Axes

Molecular Traits and Functional Analysis of the CLAVATA3/Endosperm Surrounding Region-Related Small Signaling Peptides in Three Species of Gossypium Genus

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