Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening

Gregersen, Simon; Vosch, Tom; Jensen, Knud J.

doi:10.1002/chem.201603176

Cited by 8 publications

(2 citation statements)

References 84 publications

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“…Various techniques have been developed for the synthesis of stable, water‐soluble silver NCs by using dendrimers, polymers, polypeptides, thiols, DNA, thiolated DNA, and other ligands to stabilize silver ions . The influence of various thiolates on binding energy with silver NCs has been studied in details .…”

Section: Introductionmentioning

confidence: 99%

Predicting absorption spectra of silver‐ligand complexes

Buglak

Pomogaev

Kononov

2019

Int J of Quantum Chemistry

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The detailed structures of most of ligand-stabilized metal nanoclusters (NCs) remain unknown due to the absence of crystal structure data for them. In such a situation, quantum-chemical modeling is of particular interest. We compared the performance of different theoretical methods of geometry optimization and absorption spectra calculation for silver-thiolate complexes. We showed that the absorption spectra calculated with the ADC(2) method were consistent with the spectra obtained with CC2 method. Three DFT functionals (B3LYP, CAM-B3LYP, and M06-2X) failed to reproduce the CC2 absorption spectra of the silver-thiolate complexes. K E Y W O R D SADC(2), CAM-B3LYP, CC2, M062X, silver nanoclusters, silver-thiolate complexes, TDDFT | INTRODUCTIONLigand protected metal nanoclusters (NCs) have gained much attention in the last decade due to their unique physicochemical properties. These metal NCs typically measure less than 2 nm. [1,2] Electronic, chemical, and optical properties of NCs differ from both bulk and atomic scale materials. Optically active NCs may possess strong visible or near infrared luminescence, [3] which can be used in various applications, such as bioimaging, biosensing, and optical detection of biomolecules. [4] Among the great variety of NCs, gold (Au) NCs are the most extensively investigated due to their chemical stability. [5] However, fluorescent silver (Ag) clusters are the brightest, exhibiting high quantum yield and large absorption cross-section. [6] Various techniques have been developed for the synthesis of stable, water-soluble silver NCs by using dendrimers, [7] polymers, [8] polypeptides, [9,10] thiols, [11] DNA, [12,13] thiolated DNA, [14] and other ligands to stabilize silver ions. [15] The influence of various thiolates on binding energy with silver NCs has been studied in details. [16] The nature of the ligand seems to have a strong effect on fluorescent properties of noble metal NCs. [17] Noble metal NCs protected with thiolate ligands have been of interest because of their long-term stability, which allows one to use them as building blocks constructing assembled systems with novel and improved functions. [2] Silver-thiolate and silver-amino acid complexes are studied in many laboratories nowadays both theoretically and experimentally. [18][19][20] It is assumed that silver nanoclusters are stabilized by cysteine residues in protein matrices. [21,22] While the composition of the ligand-protected NCs can be analyzed using mass-spectroscopy technique, [23] their detailed structures remain unknown in many cases. In this respect, quantum-chemical modeling of silver-ligand complexes is of particular interest. [24][25][26][27][28][29][30][31][32] Gell and coworkers investigated thiolate-protected silver NCs that contained zero, two, or four confined electrons in the cluster core. [33] They found that the number of confined electrons determine the spectroscopic patterns. For systems with two or four confined electrons, strong absorption and fluorescence in the visible or even IR energ...

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Section: Introductionmentioning

confidence: 99%

Predicting absorption spectra of silver‐ligand complexes

Buglak

Pomogaev

Kononov

2019

Int J of Quantum Chemistry

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“…[18][19][20][21] This synthetic availability combined with the predictable oligomerization of these chemically synthesized monomers, makes of gp23.1 a potentially valuable platform for bottom-up nanotechnological applications. [22][23][24][25][26]…”

Section: Introductionmentioning

confidence: 99%

Controlled Formation of Emissive Gold Nanoclusters with a Synthetic Self-Assembled Protein Toroid

Bouzada

Polo

López

et al. 2022

Preprint

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Self-assembled proteins are privileged building blocks for the bottom-up organization of matter at the nanoscale. However, since most proteins are very large, they have to be produced by recombinant expression, which is less versatile and flexible than chemical synthesis. Here, we show that we can bridge the potential of proteins for nanofabrication with the simplicity and versatility of solid-phase peptide synthesis by relying on the self-assembly of the viral protein gp23.1, a small 50-residue protein that oligomerizes in solution to form a stable toroidal hexamer. We report the chemical synthesis and basic biophysical characterization of a gp23.1 mutant and show that its self-assembled hexamer templates the formation of highly monodisperse luminescent gold nanoclusters of about 1.3 nm inside its central cavity. This work demonstrates the versatility of this small self-assembled ring protein for a variety of nanotechnological applications.

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A Simple Approach to Design Proteins for the Sustainable Synthesis of Metal Nanoclusters

et al. 2019

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Metal nanoclusters (NCs) are considered ideal nanomaterials for biological applications owing to their strong photoluminescence (PL), excellent photostability, and good biocompatibility. This study presents a simple and versatile strategy to design proteins, via incorporation of a di‐histidine cluster coordination site, for the sustainable synthesis and stabilization of metal NCs with different metal composition. The resulting protein‐stabilized metal NCs (Prot‐NCs) of gold, silver, and copper are highly photoluminescent and photostable, have a long shelf life, and are stable under physiological conditions. The biocompatibility of the clusters was demonstrated in cell cultures in which Prot‐NCs showed efficient cell internalization without affecting cell viability or losing luminescence. Moreover, the approach is translatable to other proteins to obtain Prot‐NCs for various biomedical applications such as cell imaging or labeling.

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Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening

Cited by 8 publications

References 84 publications

Predicting absorption spectra of silver‐ligand complexes

Predicting absorption spectra of silver‐ligand complexes

Controlled Formation of Emissive Gold Nanoclusters with a Synthetic Self-Assembled Protein Toroid

A Simple Approach to Design Proteins for the Sustainable Synthesis of Metal Nanoclusters

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