Peptide with Angiotensin I-Converting Enzyme Inhibitory Activity from Hydrolyzed Corn Gluten Meal

Abstract

Corn gluten meal (CGM) was hydrolyzed by Alcalase after starch removal of CGM was applied as a pretreatment. A new inhibitory peptide for angiotensin I-converting enzyme (ACE) was isolated from the hydrolysate of CGM with the use of Bio-Rad P-2 gel filtration and followed by reverse-phase high-performance liquid chromatography (RP-HPLC). The sequence of the active peptide was determined to be Ala-Tyr after the application of amino acid analysis and HPLC/MS. The IC50 of the peptide was 14.2 microM, and it was not affected by preincubation with 30 mU of ACE at 37 degrees C for 3 h. Ala-Tyr also exerted antihypertensive effects after oral administration to spontaneously hypertensive rats. A maximal reduction of systolic blood pressure of 9.5 mmHg was observed 2 h after oral administration of Ala-Tyr at doses of 50 mg/kg.

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

“…-27, -17 and -12 mmHg respectively, at the same oral dose . A maximum SBP reduction of -40 mmHg in SHRs was also observed 6 h postadministration when a tetrapeptide (TQVY) isolated from rice protein hydrolysates was orally administered at a dose of 30 mg/kg bw (Li, Qu, Wan & You, 2007), which is similar to the value obtained in this work for pentapeptides after 4 h. A relatively lower maximal SBP reduction of 9.5 mmHg in SHRs at a dose of 50 mg/kg bw 2 h post-oral administration was reported for a dipeptide AT, which was isolated from hydrolyzed corn gluten meal (Yang, Guanjun, Ping & Liu, 2007). These results indicate that the identified peptide sequences from hemp seed, especially the tetrapeptides WVYY and SVYT as well as the pentapeptides PSLPA and IPAGV represent potential ingredients that can be used to formulate antihypertensive functional foods and nutraceuticals.Howeover, dual inhibition of in vitro ACE and renin activities did not translate to better BP-reducing effects of the peptides when compared to peptides that inhibited mostly ACE activity.…”

Preparation, characterization and identification of calcium-chelating Atlantic salmon (Salmo salar L.) ossein oligopeptides

“…Amino acid composition was determined by a 835-50 automatic amino acid analyzer 1 3 (Hitachi, Ltd., Tokyo, Japan) according to a pre-established method by Yang, Tao, Liu and Liu [17].…”

Preparation of bioactive peptides with high angiotensin converting enzyme inhibitory activity from winged bean [Psophocarpus tetragonolobus (L.) DC.] seed

“…Several studies have been conducted to produce ACE inhibitory peptides by enzymatic hydrolysis of plant-derived protein rich materials such as rapeseed (Marczak et al 2003), alfalfa (Kapel et al 2006), chickpea (Yust et al 2003), red lentil (Boye et al 2010), green lentil (Akıllıoğlu and Karakaya 2009), corn gluten (Yang et al 2007) and soybean (Shin et al 2001).…”