Peptidergic Cell-Specific Synaptotagmins in<i>Drosophila</i>: Localization to Dense-Core Granules and Regulation by the bHLH Protein DIMMED

Park, Dongkook; Li, Peiyao; Dani, Adish; Taghert, Paul H.

doi:10.1523/jneurosci.2075-14.2014

Cited by 22 publications

(22 citation statements)

References 59 publications

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“…The molecular machinery for DCV fusion is still being elucidated, but is thought to have some overlap with SV pathways. For example, SNAREs are thought to be required, as well as additional members of the Synaptotagmin family, including Syta and Sytb (Adolfsen et al 2004;Park et al 2014). In addition, one wellcharacterized DCV-specific regulator of release has been identified-the Ca 2+ -activated protein for secretion (CAPS).…”

Section: Dense Core Vesicle Releasementioning

confidence: 99%

“…This transgenic line has proved highly valuable for DCV biology, revealing mechanisms of activity-dependent recruitment of DCVs (Shakiryanova et al 2005), DCV capture at active terminals (Wong et al 2012;Bulgari et al 2014), presynaptic ER-dependent Ca 2+ release driving DCV fusion (Shakiryanova et al 2007(Shakiryanova et al , 2011, and partial depletion of DCV contents upon release (Wong et al 2015). In addition to aspects of DCV release and trafficking, studies have revealed the role of the basic helix-loop-helix transcription factor Dimmed in driving neuroendocrine cell fate, including its role in regulating the expression of specific DCV proteins (Hewes et al 2006;Hamanaka et al 2010;Park et al 2011Park et al , 2014. Although the identification of the mechanisms that control DCV trafficking and fusion have lagged behind those of SV release (largely due to the ease in physiologically measuring the consequences of SV fusion), new tools in the DCV field are beginning to fill in many of the missing gaps in DCV biology.…”

Section: Dense Core Vesicle Releasementioning

confidence: 99%

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Transmission, Development, and Plasticity of Synapses

Harris¹,

2015

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Chemical synapses are sites of contact and information transfer between a neuron and its partner cell. Each synapse is a specialized junction, where the presynaptic cell assembles machinery for the release of neurotransmitter, and the postsynaptic cell assembles components to receive and integrate this signal. Synapses also exhibit plasticity, during which synaptic function and/or structure are modified in response to activity. With a robust panel of genetic, imaging, and electrophysiology approaches, and strong evolutionary conservation of molecular components, Drosophila has emerged as an essential model system for investigating the mechanisms underlying synaptic assembly, function, and plasticity. We will discuss techniques for studying synapses in Drosophila, with a focus on the larval neuromuscular junction (NMJ), a well-established model glutamatergic synapse. Vesicle fusion, which underlies synaptic release of neurotransmitters, has been well characterized at this synapse. In addition, studies of synaptic assembly and organization of active zones and postsynaptic densities have revealed pathways that coordinate those events across the synaptic cleft. We will also review modes of synaptic growth and plasticity at the fly NMJ, and discuss how pre-and postsynaptic cells communicate to regulate plasticity in response to activity. KEYWORDS FlyBook; Drosophila; synapse; neurotransmitter release; synaptic plasticity; active zone; synaptic vesicle TABLE OF CONTENTS Abstract 345Introduction 345

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Section: Dense Core Vesicle Releasementioning

confidence: 99%

Section: Dense Core Vesicle Releasementioning

confidence: 99%

Transmission, Development, and Plasticity of Synapses

Harris¹,

2015

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“…5, Extended Data Fig. 5) [24][25][26] . This is in line with previous reports of neurosecretory cells populating the dorsomedian larval brain 22 , and with ultrastructural observations, which show that cells in the dorsomedian Platynereis brain are rich in dense core vesicles while synapses are often sparse or absent 27 .…”

Section: The Apical Nervous Systemmentioning

confidence: 99%

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Achim¹,

Eling

H³

et al. 2017

Preprint

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(127 words)Animal bodies comprise a diverse array of tissues and cells. To characterise cellular identities across an entire body, we have compared the transcriptomes of single cells randomly picked from dissociated whole larvae of the marine annelid Platynereis dumerilii 1-4 . We identify five transcriptionally distinct groups of differentiated cells that are spatially coherent, as revealed by spatial mapping 5 . Besides somatic musculature, ciliary bands and midgut, we find a group of cells located at the apical tip of the animal, comprising sensory-peptidergic neurons, and another group composed of non-apical neural and epidermal cells covering the rest of the body. These data establish a basic subdivision of the larval body surface into molecularly defined apical versus non-apical tissues, and support the evolutionary conservation of the apical nervous system as a distinct part of the bilaterian brain 6 .

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“…In these cases, neuronal manipulation will likely affect the release of both biogenic amines/neuropeptides and classical transmitters. On the other hand, there is also evidence for differences between the molecular release mechanisms of amine/peptide-containing dense core vesicles and small transmitter-containing synaptic vesicles (e.g., Renden et al 2001;Park et al 2014), and biogenic amine and neuropeptide release may not be restricted to active synaptic zones (e.g., Karsai et al 2013). This may explain the inefficiency of TNTE in OA/TA neurons (Sweeney et al 1995).…”

Section: Benefits and Drawbacks Of Effector Gene Use In Larval Drosopmentioning

confidence: 99%

Potency of Transgenic Effectors for Neurogenetic Manipulation inDrosophilaLarvae

et al. 2014

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Genetic manipulations of neuronal activity are a cornerstone of studies aimed to identify the functional impact of defined neurons for animal behavior. With its small nervous system, rapid life cycle, and genetic amenability, the fruit fly Drosophila melanogaster provides an attractive model system to study neuronal circuit function. In the past two decades, a large repertoire of elegant genetic tools has been developed to manipulate and study neural circuits in the fruit fly. Current techniques allow genetic ablation, constitutive silencing, or hyperactivation of neuronal activity and also include conditional thermogenetic or optogenetic activation or inhibition. As for all genetic techniques, the choice of the proper transgenic tool is essential for behavioral studies. Potency and impact of effectors may vary in distinct neuron types or distinct types of behavior. We here systematically test genetic effectors for their potency to alter the behavior of Drosophila larvae, using two distinct behavioral paradigms: general locomotor activity and directed, visually guided navigation. Our results show largely similar but not equal effects with different effector lines in both assays. Interestingly, differences in the magnitude of induced behavioral alterations between different effector lines remain largely consistent between the two behavioral assays. The observed potencies of the effector lines in aminergic and cholinergic neurons assessed here may help researchers to choose the best-suited genetic tools to dissect neuronal networks underlying the behavior of larval fruit flies.

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Peptidergic Cell-Specific Synaptotagmins inDrosophila: Localization to Dense-Core Granules and Regulation by the bHLH Protein DIMMED

Cited by 22 publications

References 59 publications

Transmission, Development, and Plasticity of Synapses

Transmission, Development, and Plasticity of Synapses

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Potency of Transgenic Effectors for Neurogenetic Manipulation inDrosophilaLarvae

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