Peptides as a therapeutic strategy against Klebsiella pneumoniae

Souza, Camila Maurmann de; Silva, Állan Pires da; Júnior, Nelson Gomes Oliveira; Martínez, Osmel Fleitas; Franco, Octávio L.

doi:10.1016/j.tips.2021.12.006

Cited by 16 publications

(8 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent report, arginine-rich motifs of the antibiofilm activity of peptides designed for sequestering the nucleotide second messenger c-di-GMP, involved in the formation of P. aeruginosa and K. pneumoniae biofilms, were revisited [ 73 ]. The key role of the DRR and [RK] RxxD motifs in these sequestering peptides (SP) to specifically bind to c-di-GMP was demonstrated by nuclear magnetic resonance (NMR)-based experiments [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…These motifs associated with CSPs are difficult to discover using bioinformatics methods, since their sourcing peptides are probably still not registered or underrepresented in databases. However, the peptide R4F4 (RRRRFFFF), with a proven antibiofilm activity on P. aeruginosa through c-di-GMP sequestration [ 73 , 74 ], bears a more frequent motif (RRRR) among the arginine-rich ABFPs. In fact, “RRRR” was detected in our complex network-assisted motif search ( Table 5 and Table 6 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Complex Networks Analyses of Antibiofilm Peptides: An Emerging Tool for Next-Generation Antimicrobials’ Discovery

et al. 2023

View full text Add to dashboard Cite

Microbial biofilms cause several environmental and industrial issues, even affecting human health. Although they have long represented a threat due to their resistance to antibiotics, there are currently no approved antibiofilm agents for clinical treatments. The multi-functionality of antimicrobial peptides (AMPs), including their antibiofilm activity and their potential to target multiple microbes, has motivated the synthesis of AMPs and their relatives for developing antibiofilm agents for clinical purposes. Antibiofilm peptides (ABFPs) have been organized in databases that have allowed the building of prediction tools which have assisted in the discovery/design of new antibiofilm agents. However, the complex network approach has not yet been explored as an assistant tool for this aim. Herein, a kind of similarity network called the half-space proximal network (HSPN) is applied to represent/analyze the chemical space of ABFPs, aiming to identify privileged scaffolds for the development of next-generation antimicrobials that are able to target both planktonic and biofilm microbial forms. Such analyses also considered the metadata associated with the ABFPs, such as origin, other activities, targets, etc., in which the relationships were projected by multilayer networks called metadata networks (METNs). From the complex networks’ mining, a reduced but informative set of 66 ABFPs was extracted, representing the original antibiofilm space. This subset contained the most central to atypical ABFPs, some of them having the desired properties for developing next-generation antimicrobials. Therefore, this subset is advisable for assisting the search for/design of both new antibiofilms and antimicrobial agents. The provided ABFP motifs list, discovered within the HSPN communities, is also useful for the same purpose.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Complex Networks Analyses of Antibiofilm Peptides: An Emerging Tool for Next-Generation Antimicrobials’ Discovery

et al. 2023

View full text Add to dashboard Cite

show abstract

“…We believe that the plasmid data provided in this manuscript will facilitate the epidemiological surveillance of K. pneumoniae in Russia and other countries and that it presents useful data for developing prevention measures against this prominent pathogen. Such measures could possibly include the development of novel antibiotics [ 68 ] or other antimicrobial compounds like antimicrobial peptides [ 69 , 70 ], implementation of bacteriophages [ 71 ], and advancing antibiotic stewardship [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates

Shelenkov,

Mikhaylova,

Voskanyan

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Klebsiella pneumoniae, a member of the Enterobacteriaceae family, has become a dangerous pathogen accountable for a large fraction of the various infectious diseases in both clinical and community settings. In general, the K. pneumoniae population has been divided into the so-called classical (cKp) and hypervirulent (hvKp) lineages. The former, usually developing in hospitals, can rapidly acquire resistance to a wide spectrum of antimicrobial drugs, while the latter is associated with more aggressive but less resistant infections, mostly in healthy humans. However, a growing number of reports in the last decade have confirmed the convergence of these two distinct lineages into superpathogen clones possessing the properties of both, and thus imposing a significant threat to public health worldwide. This process is associated with horizontal gene transfer, in which plasmid conjugation plays a very important role. Therefore, the investigation of plasmid structures and the ways plasmids spread within and between bacterial species will provide benefits in developing prevention measures against these powerful pathogens. In this work, we investigated clinical multidrug-resistant K. pneumoniae isolates using long- and short-read whole-genome sequencing, which allowed us to reveal fusion IncHI1B/IncFIB plasmids in ST512 isolates capable of simultaneously carrying hypervirulence (iucABCD, iutA, prmpA, peg-344) and resistance determinants (armA, blaNDM-1 and others), and to obtain insights into their formation and transmission mechanisms. Comprehensive phenotypic, genotypic and phylogenetic analysis of the isolates, as well as of their plasmid repertoire, was performed. The data obtained will facilitate epidemiological surveillance of high-risk K. pneumoniae clones and the development of prevention strategies against them.

show abstract

“…LPS and CPS) of K. pneumoniae [ 142 ]. Phages, phage polysaccharide depolymerases and antimicrobial peptides represent additional promising tools for antimicrobial therapy [ 143 , 144 ]. However, most of the strategies are highly specific, even strain-dependent, thus largely comprising their cost efficiency and/or outcomes in clinical settings.…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

Autophagy, cell death, and cytokines in K. pneumoniae infection: therapeutic perspectives

Wei

Chen

et al. 2022

Emerging Microbes & Infections

View full text Add to dashboard Cite

Klebsiella pneumoniae is a notorious nosocomial pathogen causing a wide range of infections. The increasing trend of antimicrobial resistance obtained by the species immensely highly challenges the clinical treatment, representing a large threat to the global health care network. In particular, the recent convergence of multidrug resistance and hypervirulence in K. pneumoniae further worsens clinical outcomes, resulting in high mortality. Developments of new therapeutics become urgent, and immunotherapy based on antagonizing the anti-immune strategies of pathogens is a promising strategy, which requires the understanding of immune evasion mechanism in the context of the host–pathogen interactions. However, the underlying mechanisms employed by K. pneumoniae to counteract host immune responses, especially autophagy and cell death, have not been systematically reviewed and discussed yet. This review aims to summarize the tremendous progress that has been made to illuminate the landscape of cell signalling triggered by K. pneumoniae infection, especially in aspects of manipulating autophagy, cell death, and cytokine production.

show abstract

Peptides as a therapeutic strategy against Klebsiella pneumoniae

Cited by 16 publications

References 82 publications

Complex Networks Analyses of Antibiofilm Peptides: An Emerging Tool for Next-Generation Antimicrobials’ Discovery

Complex Networks Analyses of Antibiofilm Peptides: An Emerging Tool for Next-Generation Antimicrobials’ Discovery

Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates

Autophagy, cell death, and cytokines in K. pneumoniae infection: therapeutic perspectives

Contact Info

Product

Resources

About