Peptides from Harpadon nehereus protect against hyperglycemia-induced HepG2 via oxidative stress and glycolipid metabolism regulation

He, Shuang-Hui; Zhang, Xu; Li, Jie; Guo, Yueping; Lin, Qing-Fei; Jin, Huoxi

doi:10.1016/j.jff.2023.105723

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…Our results are consistent with the previous studies wherein the marine peptides mitigated oxidative stress by increasing the levels of antioxidant enzymes such as CAT, SOD, and GSH-Px [40][41][42]. It has been reported that peptides with a high proportion of hydrophobic amino acids tend to have strong ability to enhance antioxidant enzyme activities [43]. Therefore, the reason that HNCP improves the activities of antioxidant enzymes may be related to its high proportion of Pro.…”

Section: Discussionsupporting

confidence: 93%

Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism

Lin,

Guo,

et al. 2023

Marine Drugs

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Oxidative stress and abnormal glucose metabolism are the important physiological mechanisms in the occurrence and development of diabetes. Antioxidant peptides have been reported to attenuate diabetes complications by regulating levels of oxidative stress, but few studies have focused on peptides from marine bone collagen. In this study, we prepared the peptides with a molecular weight of less than 1 kD (HNCP) by enzymolysis and ultrafiltration derived from Harpadon nehereus bone collagen. Furthermore, the effects of HNCP on blood glucose, blood lipid, liver structure and function, oxidative stress, and glucose metabolism were studied using HE staining, kit detection, and Western blotting experiment in streptozocin-induced type 1 diabetes mice. After the 240 mg/kg HNCP treatment, the levels of blood glucose, triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) in streptozotocin-induced diabetes mice decreased by 32.8%, 42.2%, and 43.2%, respectively, while the levels of serum insulin and hepatic glycogen increased by 142.0% and 96.4%, respectively. The antioxidant enzymes levels and liver function in the diabetic mice were markedly improved after HNCP intervention. In addition, the levels of nuclear factor E2-related factor 2 (Nrf2), glucokinase (GK), and phosphorylation of glycogen synthase kinase-3 (p-GSK3β) in the liver were markedly up-regulated after HNCP treatment, but the glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase1 (PEPCK1) were down-regulated. In conclusion, HNCP could attenuate oxidative stress, reduce blood glucose, and improve glycolipid metabolism in streptozocin-induced type 1 diabetes mice.

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Section: Discussionsupporting

confidence: 93%

Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism

Lin,

Guo,

et al. 2023

Marine Drugs

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“…RP-HPLC is an extremely effective technology for purifying BPs according to their RT, and the RT of separated BPs can be modulated by adjusting the ratio of polar solvent (methanol and acetonitrile) in the mobile phase [50]. Therefore, RP-HPLC has been used to purify Cachelating peptides from protein hydrolysates of whey [45], casein [54], Alaska pollock [48], Mytilus edulis [50], phosvitin [55], miiuy croaker [46], tilapia (Oreochromis niloticus) [49], peanut [51], Harpadon nehereus [56], Stolephorus chinensis [57], etc. By Protein/Peptide Sequencer, the fourteen Ca-chelating peptide (ACP1-ACP14) sequences were identified as Ala-Lys (AK, ACP1), Glu-Ala-Arg (EAR, ACP2), Ala-Glu-Ala (AEA, ACP3), Val-Glu-Arg-Gly (VERG, ACP4), Val-Ala-ESr (VAS, ACP5), Gly-Pro-Lys (GPK, ACP6), Ser-Pro (SP, ACP7), Gly-Pro-Lys-Gly (GPKG, ACP8), Ala-Pro-Arg-Gly-His (APRGH, ACP9), Gly-Val-Pro-Gly (GVPG, ACP10), Leu-Glu-Pro-Gly-Pro (LEPGP, ACP11), Leu-Glu-Lys-Gly-Ala (LEKGA, ACP12), Phe-Pro-Pro-Gly-Arg (FPPGR, ACP13), and Gly-Glu-Pro-Gly (GEPG, ACP14), and their MWs were determined as 217.…”

Section: Rp-hplc Purification Of Akh-5a-2mentioning

confidence: 99%

Novel Ca-Chelating Peptides from Protein Hydrolysate of Antarctic Krill (Euphausia superba): Preparation, Characterization, and Calcium Absorption Efficiency in Caco-2 Cell Monolayer Model

Ge,

Chen,

Zhang

et al. 2023

Marine Drugs

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Antarctic krill (Euphausia superba) is the world’s largest resource of animal proteins and is thought to be a high-quality resource for future marine healthy foods and functional products. Therefore, Antarctic krill was degreased and separately hydrolyzed using flavourzyme, pepsin, papain, and alcalase. Protein hydrolysate (AKH) of Antarctic krill prepared by trypsin showed the highest Ca-chelating rate under the optimized chelating conditions: a pH of 8.0, reaction time of 50 min, temperature of 50 °C, and material/calcium ratio of 1:15. Subsequently, fourteen Ca-chelating peptides were isolated from APK by ultrafiltration and a series of chromatographic methods and identified as AK, EAR, AEA, VERG, VAS, GPK, SP, GPKG, APRGH, GVPG, LEPGP, LEKGA, FPPGR, and GEPG with molecular weights of 217.27, 374.40, 289.29, 459.50, 275.30, 300.36, 202.21, 357.41, 536.59, 328.37, 511.58, 516.60, 572.66, and 358.35 Da, respectively. Among fourteen Ca-chelating peptides, VERG presented the highest Ca-chelating ability. Ultraviolet spectrum (UV), Fourier Transform Infrared (FTIR), and scanning electron microscope (SEM) analysis indicated that the VERG-Ca chelate had a dense granular structure because the N-H, C=O and -COOH groups of VERG combined with Ca2+. Moreover, the VERG-Ca chelate is stable in gastrointestinal digestion and can significantly improve Ca transport in Caco-2 cell monolayer experiments, but phytate could significantly reduce the absorption of Ca derived from the VERG-Ca chelate. Therefore, Ca-chelating peptides from protein hydrolysate of Antarctic krill possess the potential to serve as a Ca supplement in developing healthy foods.

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“… 7 Furthermore, the peptides KLHDEEVA (KA-8) and PSRILYG (PG-7) were isolated from the hydrolysate of Harpadon nehereus bone collagen and revealed the protective effects against hyperglycemia-induced HepG2 damage ( Scheme 1 ). 8 In order to expand their application prospects and fields, it is necessary to evaluate their other biological activities such as antihypertensive and anti-inflammatory activities. Therefore, this study focuses on investigating whether they are able to protect against HUVEC injury induced by Ang II and the underlying mechanisms, including oxidative stress and dysfunction mediated by Nrf2 and AKT/nitric oxide synthase (eNOS) pathways.…”

Section: Introductionmentioning

confidence: 99%

Peptides from Harpadon nehereus Bone Ameliorate Angiotensin II-Induced HUVEC Injury and Dysfunction through Activation of the AKT/eNOS and Nrf2 Pathway

Shao,

Zhao,

Shen

et al. 2023

ACS Omega

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Angiotensin II (Ang II)-induced vascular endothelial cell injury and dysfunction are important pathophysiological factors in the occurrence and development of hypertension. In this study, the amelioration effects of two peptides KA-8 (KLHDEEVA) and PG-7 (PSRILYG) from Harpadon nehereus bone on Ang II-induced damage and dysfunction in human umbilical vein endothelial cells (HUVECs) were investigated. The results showed that they could significantly decrease the reactive oxygen species (ROS) level and increase the activity of antioxidant enzymes in Ang II-induced HUVEC. Two peptides, especially PG-7, significantly upregulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2). In addition, PG-7 significantly reduced the level of expression of endothelin-1(ET-1) and increased the phosphorylation level of phosphoinositide 3-kinase (PI3K), serine/threonine kinase (AKT), and nitric oxide synthase (eNOS). These results indicated that the two peptides, especially PG-7, can ameliorate angiotensin II-induced HUVEC injury and dysfunction through activation of the AKT/eNOS and Nrf2 pathway. Furthermore, PG-7 showed a stronger affinity with angiotensin-converting enzyme (ACE) and ACE inhibitory than KA-8. In conclusion, peptide PG-7 reveals potential in the prevention and treatment of hypertension.

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Peptides from Harpadon nehereus protect against hyperglycemia-induced HepG2 via oxidative stress and glycolipid metabolism regulation

Cited by 6 publications

References 44 publications

Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism

Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism

Novel Ca-Chelating Peptides from Protein Hydrolysate of Antarctic Krill (Euphausia superba): Preparation, Characterization, and Calcium Absorption Efficiency in Caco-2 Cell Monolayer Model

Peptides from Harpadon nehereus Bone Ameliorate Angiotensin II-Induced HUVEC Injury and Dysfunction through Activation of the AKT/eNOS and Nrf2 Pathway

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