Peptides to Overcome the Limitations of Current Anticancer and Antimicrobial Nanotherapies

Genio, Valentina Del; Bellavita, Rosa; Falanga, Annarita; Hervé-Aubert, Katel; Chourpa, Igor; Galdiero, Stefania

doi:10.3390/pharmaceutics14061235

Cited by 13 publications

(13 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, another challenging strategy used to develop antimicrobial therapeutics is the use of lipopeptides or carbohydrates to develop nanosystems that are decorated on their surface with AMPs [ 107 ].…”

Section: Applications Of Glycosylation and Lipidation To Improve Acti...mentioning

confidence: 99%

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

et al. 2023

Self Cite

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) have recently gained attention as a viable solution for combatting antibiotic resistance due to their numerous advantages, including their broad-spectrum activity, low propensity for inducing resistance, and low cytotoxicity. Unfortunately, their clinical application is limited due to their short half-life and susceptibility to proteolytic cleavage by serum proteases. Indeed, several chemical strategies, such as peptide cyclization, N-methylation, PEGylation, glycosylation, and lipidation, are widely used for overcoming these issues. This review describes how lipidation and glycosylation are commonly used to increase AMPs’ efficacy and engineer novel AMP-based delivery systems. The glycosylation of AMPs, which involves the conjugation of sugar moieties such as glucose and N-acetyl galactosamine, modulates their pharmacokinetic and pharmacodynamic properties, improves their antimicrobial activity, and reduces their interaction with mammalian cells, thereby increasing selectivity toward bacterial membranes. In the same way, lipidation of AMPs, which involves the covalent addition of fatty acids, has a significant impact on their therapeutic index by influencing their physicochemical properties and interaction with bacterial and mammalian membranes. This review highlights the possibility of using glycosylation and lipidation strategies to increase the efficacy and activity of conventional AMPs.

show abstract

Section: Applications Of Glycosylation and Lipidation To Improve Acti...mentioning

confidence: 99%

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…The concept of NFs for drug delivery is explored in our laboratory using peptide amphiphile molecules, which can self-assemble into cylindrical nanofibers easily decorated on their surface with the desired moieties for achieving the desired bio-application (for a comprehensive review on the use and advantages of peptides for anticancer therapies see ref [ 35 ]). Here, it was possible to develop NFs with Doxo and gH625, both loaded from 1 to 15 µM per 100 µM of total peptide concentration.…”

Section: Discussionmentioning

confidence: 99%

Design and Validation of Nanofibers Made of Self-Assembled Peptides to Become Multifunctional Stimuli-Sensitive Nanovectors of Anticancer Drug Doxorubicin

et al. 2022

Self Cite

View full text Add to dashboard Cite

Self-assembled peptides possess remarkable potential as targeted drug delivery systems and key applications dwell anti-cancer therapy. Peptides can self-assemble into nanostructures of diverse sizes and shapes in response to changing environmental conditions (pH, temperature, ionic strength). Herein, we investigated the development of self-assembled peptide-based nanofibers (NFs) with the inclusion of a cell-penetrating peptide (namely gH625) and a matrix metalloproteinase-9 (MMP-9) responsive sequence, which proved to enhance respectively the penetration and tumor-triggered cleavage to release Doxorubicin in Triple Negative Breast Cancer cells where MMP-9 levels are elevated. The NFs formulation has been optimized via critical micelle concentration measurements, fluorescence, and circular dichroism. The final nanovectors were characterized for morphology (TEM), size (hydrodynamic diameter), and surface charge (zeta potential). The Doxo loading and release kinetics were studied in situ, by optical microspectroscopy (fluorescence and surface-enhanced Raman scattering–SERS). Confocal spectral imaging of the Doxo fluorescence was used to study the TNBC models in vitro, in cells with various MMP-9 levels, the drug delivery to cells as well as the resulting cytotoxicity profiles. The results confirm that these NFs are a promising platform to develop novel nanovectors of Doxo, namely in the framework of TNBC treatment.

show abstract

“…20 This strategy is known as the Trojan horse approach. 21 Similarly, antimicrobial peptides (AMPs), which are widely studied and considered a promising alternative to traditional antibiotics for their mode of action, 22–28 may be linked to siderophore–iron chelators to reduce the iron availability for bacterial growth and enhance their efficacy (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of temporin L hydroxamate-based peptides and evaluation of their coordination properties with iron(iii )

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Peptides to Overcome the Limitations of Current Anticancer and Antimicrobial Nanotherapies

Cited by 13 publications

References 143 publications

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

Design and Validation of Nanofibers Made of Self-Assembled Peptides to Become Multifunctional Stimuli-Sensitive Nanovectors of Anticancer Drug Doxorubicin

Synthesis of temporin L hydroxamate-based peptides and evaluation of their coordination properties with iron(iii )

Contact Info

Product

Resources

About