Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions

Griffin, Matthew E.; Klupt, Steven; Espinosa, Juliel; Hang, Howard C.

doi:10.1016/j.chembiol.2022.11.001

Cited by 18 publications

(9 citation statements)

References 181 publications

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“…This suggests that lactobacilli in vegan samples may need and have stronger competition and survival capabilities. 82,83 Our results provide evidence that the total intestinal abundance and species of lactobacilli are affected at least by diet…”

Section: Food and Function Papermentioning

confidence: 63%

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Compositional and functional features of the intestinal lactobacilli associated with different long-term diet types

Zhang

Yin

et al. 2023

Food Funct.

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Section: Food and Function Papermentioning

confidence: 63%

“…This suggests that lactobacilli in vegan samples may need and have stronger competition and survival capabilities. 82,83…”

Section: Discussionmentioning

confidence: 99%

Compositional and functional features of the intestinal lactobacilli associated with different long-term diet types

Zhang

Yin

et al. 2023

Food Funct.

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“…Cysteine protease domains in the structural models of Lpg1355/SidG, Lpg1387, Lpg1797, and Lpg1909 share structural features with the NlpC/P60 cysteine endopeptidase family, which include known peptidoglycan (PG) degraders (Griffin et al , 2023; Hersch et al , 2020). Notably, the structurally characterized effector TseH from Vibrio cholerae translocated by type 6 secretion system (T6SS) (Altindis et al , 2015; Hersch et al ., 2020) also belongs to this protein family (Fig.…”

Section: Resultsmentioning

confidence: 99%

Global atlas of predicted functional domains inLegionella pneumophilaDot/Icm translocated effectors

Patel,

Stogios,

Jaroszewski

et al. 2024

Preprint

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Legionella pneumophila utilizes the Dot/Icm type IVB secretion system to deliver hundreds of effector proteins inside eukaryotic cells to ensure intracellular replication. Our understanding of the molecular functions of the largest pathogenic arsenal known to the bacterial world remains incomplete. By leveraging advancements in 3D protein structure prediction, we provide a comprehensive structural analysis of 368 L. pneumophila effectors, representing a global atlas of predicted functional domains summarized in a database (https://pathogens3d.org/legionella-pneumophila). Our analysis identified 157 types of diverse functional domains in 287 effectors, including 159 effectors with no prior functional annotations. Furthermore, we identified 35 unique domains in 30 effector models that have no similarity with experimentally structurally characterized proteins, thus, hinting at novel functionalities. Using this analysis, we demonstrate the activity of thirteen domains, including three unique folds, predicted in L. pneumophila effectors to cause growth defects in the Saccharomyces cerevisiae model system. This illustrates an emerging strategy of exploring synergies between predictions and targeted experimental approaches in elucidating novel effector activities involved in infection.

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“…95 Thus, PiuB could be responsible for a broad range of effects caused by proteolytic processing of surface proteins and cell wall hydrolases, which are often regulated through cleavage events. 73 Interestingly, three relatively uncharacterized proteins are also increased at the cell surface exclusively Hb-enhanced.…”

Section: Discussionmentioning

confidence: 99%

The exoproteome and surfaceome of toxigenicCorynebacterium diphtheriae1737 and its response to iron-restriction and growth on human hemoglobin

Goring,

Hale,

Dasika

et al. 2024

Preprint

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Toxin-producing Corynebacterium diphtheriae strains are the etiological agent of the severe upper respiratory disease, diphtheria. A global phylogenetic analysis revealed that biotype gravis is particularly lethal as it produces diphtheria toxin and a range of other virulence factors, particularly when it encounters low levels of iron at sites of infection. To gain insight into how it colonizes its host we have identified iron-dependent changes in the exoproteome and surfaceome of C. diphtheriae strain 1737 using a combination of whole-cell fractionation, intact cell surface proteolysis, and quantitative proteomics. In total, we identified 1,425 of the predicted 2,265 (63%) proteins encoded by its reference genome. For each protein we quantified its degree of secretion and surface-exposure, revealing that exoproteases and hydrolases predominate in the exoproteome, while the surfaceome is enriched with adhesins, particularly DIP2093. Our analysis provides insight into how components in the heme-acquisition system are positioned, showing pronounced surface-exposure of the strain-specific ChtA/ChtC paralogues and high secretion of the species-conserved heme-binding HtaA protein suggesting it functions as a hemophore. Profiling the response of the exoproteome and surfaceome after microbial exposure to human hemoglobin and iron limitation reveals potential virulence factors that may be expressed at sites of infection. Data are available via ProteomeXchange with identifier PXD051674.

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Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions

Cited by 18 publications

References 181 publications

Compositional and functional features of the intestinal lactobacilli associated with different long-term diet types

Compositional and functional features of the intestinal lactobacilli associated with different long-term diet types

Global atlas of predicted functional domains inLegionella pneumophilaDot/Icm translocated effectors

The exoproteome and surfaceome of toxigenicCorynebacterium diphtheriae1737 and its response to iron-restriction and growth on human hemoglobin

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