Peptidomics in Neuroendocrine Research: A
            <i>Caenorhabditis elegans</i>
            and
            <i>Mus musculus</i>
            Study

Boonen, Kurt; Husson, Steven; Baggerman, Geert; Cerstiaens, Anja; Luyten, Walter; Schoofs, Liliane

doi:10.1002/9780470196502.ch16

Cited by 1 publication

(3 citation statements)

References 47 publications

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“…Boonen et al used acidified methanol, this mixture will rapidly denaturate proteolytic enzymes when combined with homogenization. Acidified methanol has the additional advantage of sensitivity; onefourth of a pituitary was sufficient for a successful peptidomics experiment [148]. A recent study of Dowell et al [150] combined the use of cryostat dissection and a boiling extraction buffer to prevent proteolysis.…”

Section: Peptide Identification By Ms and Hyphenated Techniquesmentioning

confidence: 98%

“…Although already very efficient, peptidomics requires additional careful handling of the tissue. Proteases, as well as peptidases, have to be inactivated [148]. This can be done chemically (high and low pH, chaotropic substances, protease inhibitors and denaturating solvents) or physically (high or low temperatures) and combinations of these methods are usually sufficient.…”

Section: Peptidomics: the Datamentioning

confidence: 98%

“…This approach by Svensson et al [172] showed improved neuropeptide detection. Boonen et al [148] developed a second approach that used rapid cooling of the mouse brain tissue (pituitary) with liquid nitrogen. Further careful sample handling prevented proteolysis to occur.…”

Section: Peptide Identification By Ms and Hyphenated Techniquesmentioning

confidence: 99%

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Peptidomics: The integrated approach of MS, hyphenated techniques and bioinformatics for neuropeptide analysis

Boonen¹,

Landuyt

Baggerman

et al. 2008

J of Separation Science

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MS is currently one of the most important analytical techniques in biological and medical research. ESI and MALDI launched the field of MS into biology. The performance of mass spectrometers increased tremendously over the past decades. Other technological advances increased the analytical power of biological MS even more. First, the advent of the genome projects allowed an automated analysis of mass spectrometric data. Second, improved separation techniques, like nanoscale HPLC, are essential for MS analysis of biomolecules. The recent progress in bioinformatics is the third factor that accelerated the biochemical analysis of macromolecules. The first part of this review will introduce the basics of these techniques. The field that integrates all these techniques to identify endogenous peptides is called peptidomics and will be discussed in the last section. This integrated approach aims at identifying all the present peptides in a cell, organ or organism (the peptidome). Today, peptidomics is used by several fields of research. Special emphasis will be given to the identification of neuropeptides, a class of short proteins that fulfil several important intercellular signalling functions in every animal. MS imaging techniques and biomarker discovery will also be discussed briefly.

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Section: Peptide Identification By Ms and Hyphenated Techniquesmentioning

confidence: 98%

Section: Peptidomics: the Datamentioning

confidence: 98%