Peptoniphilus coli sp. nov. and Peptoniphilus urinae sp. nov., isolated from humans

Abstract

Strains Marseille-P3761 and Marseille-P3195 are representatives of two bacterial species isolated from human specimens. Strain Marseille-P3761 was isolated from the stool of a healthy volunteer, while strain Marseille-P3915 was cultivated from the urine of a kidney transplant recipient. Both strains are anaerobic Gram-positive coccoid bacteria. Both are catalase-negative and oxidase-negative and grow optimally at 37 °C in anaerobic conditions. They also metabolize carbohydrates, such as galactose, glucose, fructose, and glycerol. The major fatty acids were hexadecanoic acid for both strains. The highest digital DNA–DNA hybridization (dDDH) values of Marseille-P3761 and Marseille-P3195 strains when compared to their closest phylogenetic relatives were 52.3% and 56.4%, respectively. Strains Marseille-P3761 and Marseille-P3195 shared an OrthoANI value of 83.5% which was the highest value found with Peptoniphilus species studied here. The morphological, biochemical, phenotypic and genomic characteristics strongly support that these strains are new members of the Peptoniphilus genus. Thus, we suggest that Peptoniphilus coli sp. nov., and Peptoniphilus urinae sp. nov., are new species for which strains Marseille-P3761 (CSUR P3761 = CCUG 71,569) and Marseille-P3195 (CSUR P3195 = DSM 103,468) are their type strains, respectively of two new Peptoniphilus species, for which we propose the names Peptoniphilus coli sp. nov. and Peptoniphilus urinae sp. nov., respectively.

Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse

“…The phenotypic characteristics of CBA3646 T and those of the reference strains P. asaccharolyticus KCTC 3321 3). C 16 : 0 , one of the major fatty acids of CBA3646 T , has also been shown to be the major fatty acid of strains of members of the genus Peptoniphilus [13][14][15][16][17][18], indicating that CBA3646 T shares cellular properties with members of the genus Peptoniphilus. C 14 : 0 was a major fatty acid in P. asaccharolyticus KCTC 3321 T but was a minor fatty acid in CBA3646 T .…”

“…its species were formerly classified as members of the genus Peptostreptococcus) in 2001 on the basis of the sequence of the 16S rRNA gene [8,12]. Species of the genus Peptoniphilus have been successfully isolated from various human clinical specimens [13][14][15][16][17] and swine faeces [18,19]. Bacteria of the genus Peptoniphilus are Gram-stain-positive, non-motile, anaerobic cocci.…”