Disinfection of surface drinking water, in particular water chlorination, results in many by-products with potential genotoxic and/or carcinogenic activity. In the present study, we evaluated the genotoxicity of surface water after treatment with different disinfectants by means of in situ plant genotoxicity assays (micronucleus and chromosomal aberration tests) which can detect both clastogenic and aneugenic effects. The study was carried out at a pilot plant using lake water after sedimentation and filtration. This water supplied four stainless steel basins: three basins were disinfected with sodium hypochlorite, chlorine dioxide, and peracetic acid and the fourth basin containing untreated lake water was used as a control. Plants were exposed in situ in the basins. The study was carried out using water collected in different seasons over a period of about 1 year in order to assess the treatments in different physical and chemical lake water conditions. The micronucleus test in root cells of Vicia faba (Vicia faba/MCN test) revealed genotoxicity in many samples of disinfected water. The micronucleus test in Tradescantia pollen cells and the chromosome aberration test in root cells of Allium cepa showed genotoxic effects only in some disinfected samples, but also revealed genotoxicity in raw water. The results of the study indicated that the Vicia faba/ MCN test was the most sensitive plant assay for disinfected water and that peracetic acid disinfection produced similar or lower genotoxicity than sodium hypochlorite or chlorine dioxide treatment.
INTRODUCTIONDrinking water disinfection may produce toxic compounds, particularly if the water is obtained from surface sources. Water chlorination results in mutagenic/carcinogenic by-products derived from the reaction of chlorine with organic compounds (humic and fulvic acids) naturally present in water [Rook, 1974; WHO, 1996]. For this reason it is very important to test alternative disinfectants to chlorine in order to reduce these potential health risks. Among the new disinfectants, peracetic acid (CH 3 -CO-OOH, PAA) deserves to be studied for its application in drinking water disinfection, since it is a potent antimicrobial agent and has many applications in hospitals, laboratories, and factories [Baldry et al., 1991[Baldry et al., , 1995Lefevre et al., 1992]. More recently, PAA has been found to be an effective biocidal compound for wastewater disinfection, and previous research showed that disinfection of lake and river drinking water with PAA gave rise to a very low level of genotoxicity and produced only carboxylic acids [Monarca et al., 2002a], which are not recognized as mutagenic.Drinking water has been intensively examined for gene mutations, primarily by assaying extracts of water in bacterial tests, and several studies on different disinfectants have been carried out [Koivusalo et al., 1994;Loper et al., 1980;Monarca 1985Monarca , 1998Monarca , 2002aRomero et al., 1992;Tuomisto et al., 1990;Wilcox and Williamson, 1986;Wilcox et al., 1988]. Plant genot...