Percentage of phagocytosis, production of O2·−, H2O2 and NO, and antioxidant enzyme activities of rat neutrophils in culture

Curi, Tânia Cristina Pithon; Melo, Mariza Pires de; Palanch, Adrianne C.; Miyasaka, Célio Kenji; Curi, Rui

doi:10.1002/(sici)1099-0844(199803)16:1<43::aid-cbf761>3.0.co;2-5

Cited by 28 publications

(7 citation statements)

References 22 publications

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“…25,26 The pentose phosphate pathway is instrumental in the formation of NADPH and ribose-5-phosphate for fatty acid and nucleotide synthesis, respectively. 27 NADPH is important for NADPH oxidase activity and for the rejuvenation of glutathione in neutrophils; 28 and activation of NADPH oxidase results in a respiratory burst in neutrophils during the process of phagocytosis. 29 In diabetic patients, NADPH production is decreased, which leads eventually to compromised neutrophil function.…”

Section: Diabetes-induced Systemic Infl Ammatory Statementioning

confidence: 99%

Interrelationship between Diabetes and Periodontitis- A Review

Pradhan

Goel

2011

J Nepal Med Assoc

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Evidence for the link between periodontal disease and several systemic diseases is growing rapidly. Diabetes mellitus is a systemic disease with several major complications affecting both the quality and length of life causing morbidity and mortality. Periodontitis, one of these complications, is a chronic infection associated with substantial morbidity in the form of tooth loss and that affects the quality of life directly. The association between diabetes and inflammatory periodontal disease has been studied extensively. The relationship between these two conditions appears bidirectional. The presence of one condition tends to promote the other and the meticulous management of either may assist treatment of the other. It also provides a perfect example of a cyclical association, whereby a systemic disease predisposes the individual to oral infections, and once the oral infection is established, it exacerbates the systemic disease. This review focuses to explain the interrelationship between the two based on information in the literature and the potential common immunoregulatory connections involved, exploring the mechanisms through which periodontal infection can contribute to the low-grade general inflammation associated with diabetes. Keywords: Diabetes mellitus; inflammation; insulin resistance; periodontitis.

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Section: Diabetes-induced Systemic Infl Ammatory Statementioning

confidence: 99%

Interrelationship between Diabetes and Periodontitis- A Review

Pradhan

Goel

2011

J Nepal Med Assoc

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“…An increase in the rate of the respiratory burst involves a sudden stimulus-induced increase in nonmitochondrial oxidative metabolism which results in the production of the superoxide anion (O − : # ) and associated reactive oxygen species [1,2]. The combination of these processes results in the killing and digestion of the engulfed bacteria and, if prolonged, the development of a local inflam-mation.…”

Section: Introductionmentioning

confidence: 99%

Effects of adrenaline on glucose and glutamine metabolism and superoxide production by rat neutrophils

et al. 1999

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Despite the large body of information on the role of corticosteroids in regulating lymphocyte and phagocyte function, the role of the hormone adrenaline in immunoregulation is an under-investigated topic. The present study has addressed the effects of adrenaline on the rates of utilization and oxidation of glucose and glutamine, the phagocytic capacity and the rate of superoxide production by rat neutrophils. Incubation of rat neutrophils in the presence of 50 microM adrenaline caused a marked elevation in glucose metabolism, an effect that could be blocked by propranolol. Adrenaline caused a partial inhibition of glutamine utilization by neutrophils, an effect that was also blocked by propranolol. These effects of adrenaline could be mimicked by 100 microM dibutyryl cAMP. Phosphate-dependent glutaminase activity was significantly elevated in neutrophils incubated in the presence of 50 microM adrenaline or 100 microM dibutyryl cAMP for 1 h, whereas glutamine oxidation was significantly depressed (P<0.05) under these conditions. The elevation in enzyme activity was only partially blocked by propranolol. The phagocytic activity of rat neutrophils was not altered by adrenaline in the presence of either glucose or glutamine. The rate of phorbol 12-myristate 13-acetate-induced superoxide production in the presence of glucose was potently reduced by the addition of 5 nM or 50 microM adrenaline. This effect could be mimicked by dibutyryl cAMP. However, when rat neutrophils were incubated in the presence of glutamine plus adrenaline (5 nM or 50 microM), the rate of superoxide production was only marginally reduced. These findings support the proposition that adrenaline may deviate the flux of glucose from the NADPH-producing pentose phosphate pathway, thus reducing substrate availability for the superoxide-generating NADPH oxidase. However, glutamine metabolism may still give rise to substantial quantities of NADPH from the glutaminolysis pathway. We postulate that glutamine metabolism may thus provide a protective mechanism against the inhibitory effect of adrenaline on superoxide production by neutrophils.

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“…25 Phagocytosis of opsonized zymosan was performed with adaptations described elsewhere. 26 Briefly, heparinized blood (15 U mL −1 ) was mixed with Histopaque (Sigma-Aldrich, St Louis, Missouri, USA) and then centrifuged at 400 g for 30 min at room temperature. Polymorphonuclears were separated in the bottom of centrifugation tube and then submitted to hypotonic hemolysis.…”

Section: Methodsmentioning

confidence: 99%

Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom

et al. 2015

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Loxosceles gaucho spider venom induces in vitro platelet activation and marked thrombocytopenia in rabbits. Herein, we investigated the involvement of platelets in the development of the dermonecrosis induced by L. gaucho venom, using thrombocytopenic rabbits as a model. L. gaucho venom evoked a drop in platelet and neutrophil counts 4 h after venom injection. Ecchymotic areas at the site of venom inoculation were noticed as soon as 4 h in thrombocytopenic animals but not in animals with initial normal platelet counts. After 5 days, areas of scars in thrombocytopenic animals were also larger, evidencing the marked development of lesions in the condition of thrombocytopenia. Histologically, local hemorrhage, collagen fiber disorganization, and edema were more severe in thrombocytopenic animals. Leukocyte infiltration, predominantly due to polymorphonuclears, was observed in the presence or not of thrombocytopenia. Thrombus formation was demonstrated by immunohistochemistry at the microvasculature, and it occurred even under marked thrombocytopenia. Taken together, platelets have an important role in minimizing not only the hemorrhagic phenomena but also the inflammatory and wound-healing processes, suggesting that cutaneous loxoscelism may be aggravated under thrombocytopenic conditions.

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Percentage of phagocytosis, production of O2·−, H2O2 and NO, and antioxidant enzyme activities of rat neutrophils in culture

Cited by 28 publications

References 22 publications

Interrelationship between Diabetes and Periodontitis- A Review

Interrelationship between Diabetes and Periodontitis- A Review

Effects of adrenaline on glucose and glutamine metabolism and superoxide production by rat neutrophils

Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom

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