Background/Aims Mothers may experience significant stress if their baby is admitted to the neonatal unit, especially if they are uncertain about what is happening, and may use various coping mechanisms to manage stress. Ineffective support systems can lead to emotional, social and financial strain for women in this situation. Maternal health is paramount not only for a woman's own sake but also because a healthy mother is better placed to care for a sick baby. This study sought to determine factors related to stress, and the coping mechanisms and support systems for mothers whose babies had neonatal sepsis. Methods This cross-sectional mixed-method study involved 78 purposively selected mothers of babies with neonatal sepsis. Data were collected between 15 June 2023 and 6 July 2023 in five hospital wards, using a researcher-administered semi-structured questionnaire. Data were analysed using descriptive and inferential statistics for quantitative data and thematic analysis for qualitative data. Results Participants commonly experienced high (25%) or moderate (71%) stress, and used psychological or social methods to cope, in addition to engaging in calming activities such as reading or singing. Participants' main source of support was social, from family, friends and medical professionals. Conclusions Mothers of babies with neonatal sepsis are likely to experience high stress levels and require psychological, social and financial support from their family, church and the hospital. Implications for practice Healthcare workers should closely monitor the mothers of admitted neonates to identify those who may need psychological care. Mothers should be reassured and kept informed about the progress of their babies.