Citizenship participation in the empowerment of local potential is a prerequisite for every development plan. Community-based ecotourism development (CBED) involves the resources and unique characteristics of the local community, including physical and nonphysical components (values, norms, customs, and traditions). The aims of this research are (1) to describe the role of Isy Karima Islamic Boarding School in developing religious ethics; (2) to describe a mechanism for developing citizenship participation; (3) to map the aspects of citizenship participation in sustainable ecotourism development; (4) to analyze the stages of citizenship participation in sustainable ecotourism development; and ( 5) to analyze the activities of stakeholders in building sustainability. The research method used was qualitative descriptive, with a sociological anthropological approach. Three surrounding villages in the District of Karangpandan were chosen through purposive sampling to characterize the participation of local citizens in CBED. The data obtained were then analyzed using a single case analysis. The results show that Isy Karima Boarding School holds various activities for the empowerment of MSMEs, one of which is the development of partnership networks. Citizenship participation in the community around Isy Karima Boarding School in sustainable tourism development has grown and developed through a mechanism of awareness, training, counselling and guidance, and execution. The activities of stakeholders in building sustainable ecotourism have an impact on economic, socio-cultural, and political aspects. This impact can provide a basic reference for an ecotourism development model that includes community participation.