Perchlorate bioreduction linked to methane oxidation in a membrane biofilm reactor: Performance and microbial community structure

Xie, Ting; Yang, Qi; Winkler, Mari K.H.; Wang, Dongbo; Zhong, Yu; An, Hongxue; Chen, Fei; Yao, Fubin; Wang, Xiaolin; Wu, Jiawei; Li, Xiaoming

doi:10.1016/j.jhazmat.2018.06.011

Cited by 42 publications

(20 citation statements)

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“…Although it is close to impossible to predict the actual substrates of the NarG LFLS (or PcrA LFLS ), the phylogenetic analysis is, at least, suggestive of the potential utilisation of (per)chlorate. The oxidation of methane linked to the bioreduction of perchlorate has been proposed a number of times in the past ( Luo et al, 2015 ; Xie et al, 2018 ; Wu et al, 2019 ). Recent works ( Xie et al, 2018 ; Wu et al, 2019 ) have suggested the possible involvement of ANME archaea in (per)chlorate reduction based on chemical and amplicon data analyses, an assumption considered more likely given that nitrate was rarely measured in noticeable concentrations in the LFLS sampling trench ( Vázquez-Campos et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

et al. 2021

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During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population dynamics in a waste trench during a rainfall event have been previously investigated revealing a broad abundance of candidate and potentially undescribed taxa in this iron-rich, radionuclide-contaminated environment. Applying genome-based metagenomic methods, we recovered 37 refined archaeal MAGs, mainly from undescribed DPANN Archaea lineages without standing in nomenclature and ‘Candidatus Methanoperedenaceae’ (ANME-2D). Within the undescribed DPANN, the newly proposed orders ‘Ca. Gugararchaeales’, ‘Ca. Burarchaeales’ and ‘Ca. Anstonellales’, constitute distinct lineages with a more comprehensive central metabolism and anabolic capabilities within the ‘Ca. Micrarchaeota’ phylum compared to most other DPANN. The analysis of new and extant ‘Ca. Methanoperedens spp.’ MAGs suggests metal ions as the ancestral electron acceptors during the anaerobic oxidation of methane while the respiration of nitrate/nitrite via molybdopterin oxidoreductases would have been a secondary acquisition. The presence of genes for the biosynthesis of polyhydroxyalkanoates in most ‘Ca. Methanoperedens’ also appears to be a widespread characteristic of the genus for carbon accumulation. This work expands our knowledge about the roles of the Archaea at the LFLS, especially, DPANN Archaea and ‘Ca. Methanoperedens’, while exploring their diversity, uniqueness, potential role in elemental cycling, and evolutionary history.

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Section: Discussionmentioning

confidence: 99%

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

et al. 2021

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“…Although it is close to impossible to predict the actual substrates of the NarG LFLS (or PcrA LFLS ), the phylogenetic analysis is, at least, suggestive of the potential utilisation of (per)chlorate. The oxidation of methane linked to the bioreduction of perchlorate has been proposed a number of times in the past (Luo et al, 2015; Xie et al, 2018; Wu et al, 2019). Recent works (Xie et al, 2018; Wu et al, 2019) have suggested the possible involvement of ANME archaea in (per)chlorate reduction based on chemical and amplicon data analyses, an assumption considered more likely given that nitrate was rarely measured in noticeable concentrations in the LFLS sampling trench (Vázquez-Campos et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Genomic insights into the Archaea inhabiting an Australian radioactive legacy site

Vázquez-Campos

Kinsela

Bligh

et al. 2019

Preprint

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18During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at 19 the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population 20 dynamics during a rainfall event using shotgun metagenomics has been previously investigated. This 21 revealed a broad abundance of candidate and potentially undescribed taxa in this iron-rich, 22 radionuclide-contaminated environment. 23Here, applying genome-based metagenomic methods, we recovered 37 refined archaeal bins (≥50% 24 completeness, ≤ 10% redundancy) from 10 different major lineages. They were, for the most part, 25 included in 4 proposed lineages within the DPANN supergroup (LFWA-I to IV) and 26 Methanoperedenaceae. 27The new Methanoperedens spp. bins, together with previously published data, suggests a potentially 28 widespread ability to use nitrate (or nitrite) and metal ions as electron acceptors during the anaerobic 29 oxidation of methane by Methanoperedens spp. 30 While most of the new DPANN lineages show reduced genomes with limited central metabolism 31 typical of other DPANN, the candidate species from the proposed LFWA-III lineage show some 32 unusual features not often present in DPANN genomes, i.e. a more comprehensive central metabolism 33 and anabolic capabilities. While there is still some uncertainty about the capabilities of LFW-121_3 34 and closely related archaea for the biosynthesis of nucleotides de novo and amino acids, it is to date 35 the most promising candidate to be a bona fide free-living DPANN archaeon. 36 environments such as rice fields and ocean floors before it is released into the atmosphere [12]. Our 64 previous research showed that the archaeal community in the LFLS trenches was mainly composed of 65 methanogens and anaerobic methane oxidisers (ANME-2d) from the Methanomicrobia class and 66 DPANN archaea [3]. While they constituted a minor component of the community, they still may 67 have an important role in facilitating redox cycling and therefore impacting upon contaminant 68 mobilisation. 69 Here we describe the analysis of 37 new archaeal genomes with different degrees of completeness, 70 derived from samples collected in a legacy radioactive waste trench at the LFLS during a redox 71 cycling event. We present evidence of four new DPANN lineages and six non-conspecific 72 Methanoperedens sp. genomes, while exploring their potential role in the biogeochemical cycles and 73 uniqueness. 74 Material and Methods 75 Data source and experimental details 76 Raw sequencing reads from ENA project PRJEB14718 [3] were reanalysed, this time using 77 genome-based metagenomic methodologies in order to better understand the contributions of the 78 Archaea in the LFLS groundwater to the biogeochemistry of the site. Briefly, samples were collected 79 in triplicate over a period of 47 days at 4 time-points (0, 4, 21 and 47 days) after an intense rainfall 80 event that completely filled the trench. Thorough chemical and radiochemical analyses were 81 conducted on the samples in...

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“…In situ and ex situ decontamination processes for perchlorate have primarily focused on biological technologies based on the use of PRB [27,31,32] since they do not generate toxic products and are also practical to use. The possibility of employing these microorganisms in bioremediation plans is connected to the knowledge of their physiology and of the main environmental factors that regulate the respiration of perchlorate.…”

Section: Perchlorate (Clomentioning

confidence: 99%

Preferential Use of the Perchlorate over the Nitrate in the Respiratory Processes Mediated by the Bacterium Azospira sp. OGA 24

Guarino

Motta

Turano

et al. 2020

Water

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Here we report the results obtained for a strain isolated from a polluted site and classified as Azospira sp. OGA 24. The capability of OGA24 to utilize perchlorate and nitrate and the regulation of pathways were investigated by growth kinetic studies and analysis of messenger RNA (mRNA) expression of the genes of perchlorate reductase alpha subunit (pcrA), chlorite dismutase (cld), and periplasmic nitrate reductase large subunit (napA). In aerobic conditions and in a minimal medium containing 10 mM acetate as carbon source, 5.6 ± 0.34 mmol L−1 perchlorate or 9.7 ± 0.22 mmol L−1 nitrate were efficiently reduced during the growth with 10 mM of either perchlorate or nitrate. In anaerobiosis, napA was completely inhibited in the presence of perchlorate as the only electron acceptor, pcrA was barely detectable in nitrate-reducing conditions. The cell growth kinetics were in accordance with expression data, indicating a separation of nitrate and perchlorate respiration pathways. In the presence of both compounds, anaerobic nitrate consumption was reduced to 50% (4.9 ± 0.4 vs. 9.8 ± 0.15 mmol L−1 without perchlorate), while that of perchlorate was not affected (7.2 ± 0.5 vs. 6.9 ± 0.6 mmol L−1 without nitrate). Expression analysis confirmed the negative effect of perchlorate on nitrate respiration. Based on sequence analysis of the considered genes and 16S ribosomal gene (rDNA), the taxonomic position of Azospira sp. OGA24 in the perchlorate respiring bacteria (PRB) group was further defined by classifying it in the oryzae species. The respiratory characteristics of OGA24 strain make it very attractive in terms of potential applications in the bioremediation of environments exposed to perchlorate salts.

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Perchlorate bioreduction linked to methane oxidation in a membrane biofilm reactor: Performance and microbial community structure

Cited by 42 publications

References 53 publications

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site

Genomic insights into the Archaea inhabiting an Australian radioactive legacy site

Preferential Use of the Perchlorate over the Nitrate in the Respiratory Processes Mediated by the Bacterium Azospira sp. OGA 24

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