Perchlorate reduction by an isolated<i>Serratia marcescens</i>strain under high salt and extreme pH

Nadaraja, Anupama Vijaya; Veetil, Prajeesh Gangadharan Puthiya; Krishnakumar, B.

doi:10.1111/1574-6968.12062

Cited by 17 publications

(4 citation statements)

References 33 publications

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“…While continued isolation of novel DPRB provides a diverse collection of bacteria capable of this metabolism (15–17), understanding the basic tenets of perchlorate reduction is hindered by the lack of a robust model system amenable to genetic manipulation. To fill this role, we have selected the betaproteobacterium Azospira suillum PS (formerly known as Dechlorosoma suillum ) (18).…”

Section: Introductionmentioning

confidence: 99%

Transposon and Deletion Mutagenesis of Genes Involved in Perchlorate Reduction in Azospira suillum PS

Melnyk

Clark

Liao

et al. 2014

mBio

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Although much work on the biochemistry of the key enzymes of bacterial perchlorate reduction, chlorite dismutase, and perchlorate reductase has been published, understanding of the molecular mechanisms of this metabolism has been somewhat hampered by the lack of a clear model system amenable to genetic manipulation. Using transposon mutagenesis and clean deletions, genes important for perchlorate reduction in Azospira suillum PS have been identified both inside and outside the previously described perchlorate reduction genomic island (PRI). Transposon mutagenesis identified 18 insertions in 11 genes that completely abrogate growth via reduction of perchlorate but have no phenotype during denitrification. Of the mutants deficient in perchlorate reduction, 14 had insertions that were mapped to eight different genes within the PRI, highlighting its importance in this metabolism. To further explore the role of these genes, we also developed systems for constructing unmarked deletions and for complementing these deletions. Using these tools, every core gene in the PRI was systematically deleted; 8 of the 17 genes conserved in the PRI are essential for perchlorate respiration, including 3 genes that comprise a unique histidine kinase system. Interestingly, the other 9 genes in the PRI are not essential for perchlorate reduction and may thus have unknown functions during this metabolism. We present a model detailing our current understanding of perchlorate reduction that incorporates new concepts about this metabolism.

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Section: Introductionmentioning

confidence: 99%

Transposon and Deletion Mutagenesis of Genes Involved in Perchlorate Reduction in Azospira suillum PS

Melnyk

Clark

Liao

et al. 2014

mBio

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“…The genera Nesiotobacter and Salinivibrio showed the highest percentage (25%) of perchlorate reduction, while the genera Vibrio , Bacillus , and Staphylococcus presented a lowest proportion of KClO 4 reduction, with 14, 12, and 10%, respectively. Recent studies have shown that the amount of perchlorate reduced may be inversely proportional to increased salinity [13, 17]. Future studies should be carried out to describe the role of salinity on perchlorate reduction by these strains.…”

Section: Resultsmentioning

confidence: 99%

“…Marine soils usually contain bacterial species with biochemical versatility and ability to tolerate salt, being an interesting target for researchers due to the potential reduction of environmental perchlorate [10]. The reason for selecting this type of environment is that degradation of perchlorate may be carried out using salt-tolerant bacteria [12], although this perchlorate-reduction process could be impaired with increasing salinity [11, 13]. Moreover, these organisms are available in diverse environments, from Antarctica, saline lakes, and hot springs, even in hyperthermophilic and hypersaline soils [10, 11].…”

Section: Introductionmentioning

confidence: 99%

Perchlorate-Reducing Bacteria from Hypersaline Soils of the Colombian Caribbean

Acevedo-Barrios

Bertel-Sevilla

Alonso

et al. 2019

International Journal of Microbiology

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Perchlorate (ClO4−) has several industrial applications and is frequently detected in environmental matrices at relevant concentrations to human health. Currently, perchlorate-degrading bacteria are promising strategies for bioremediation in polluted sites. The aim of this study was to isolate and characterize halophilic bacteria with the potential for perchlorate reduction. Ten bacterial strains were isolated from soils of Galerazamba-Bolivar, Manaure-Guajira, and Salamanca Island-Magdalena, Colombia. Isolates grew at concentrations up to 30% sodium chloride. The isolates tolerated pH variations ranging from 6.5 to 12.0 and perchlorate concentrations up to 10000 mg/L. Perchlorate was degraded by these bacteria on percentages between 25 and 10. 16S rRNA gene sequence analysis indicated that the strains were phylogenetically related to Vibrio, Bacillus, Salinovibrio, Staphylococcus, and Nesiotobacter genera. In conclusion, halophilic-isolated bacteria from hypersaline soils of the Colombian Caribbean are promising resources for the bioremediation of perchlorate contamination.

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“…Marine soils usually contain bacteria species with biochemical versatility and ability to tolerate salt, being an interesting target for researchers due to the potential reduction of environmental perchlorate (Logan et al 2001). The reason for selecting this type of environment is that degradation of perchlorate may be carried out using salt-tolerant bacteria (Okeke et al 2002), although this perchlorate-reduction process could be impaired with increasing salinity (Vijaya Nadaraja et al 2013;Matsubara et al 2016).…”

Section: Habitats Of Perchlorate-reducing Bacteriamentioning

confidence: 99%

Perchlorate Contamination: Sources, Effects, and Technologies for Remediation

Acevedo-Barrios

Olívero-Verbel

2021

Reviews of Environmental Contamination and Toxicology

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Perchlorate is a persistent pollutant, generated via natural and anthropogenic processes, that possesses a high potential for endocrine disruption in humans and biota. It inhibits iodine fixation, a major reason for eliminating this pollutant from ecosystems. Remediation of perchlorate can be achieved with various physicochemical treatments, especially at low concentrations. However, microbiological approaches using microorganisms, such as those from the genera Dechloromonas, Serratia, Propionivibrio, Wolinella, and Azospirillum, are promising when perchlorate pollution is extensive. Perchlorate-reducing bacteria, isolated from harsh

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Perchlorate reduction by an isolatedSerratia marcescensstrain under high salt and extreme pH

Cited by 17 publications

References 33 publications

Transposon and Deletion Mutagenesis of Genes Involved in Perchlorate Reduction in Azospira suillum PS

Transposon and Deletion Mutagenesis of Genes Involved in Perchlorate Reduction in Azospira suillum PS

Perchlorate-Reducing Bacteria from Hypersaline Soils of the Colombian Caribbean

Perchlorate Contamination: Sources, Effects, and Technologies for Remediation

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