Perchlorate reduction by hydrogen autotrophic bacteria and microbial community analysis using high-throughput sequencing

Wan, Dongjin; Liu, Yongde; Niu, Zhenhua; Xiao, Shuhu; Li, Daorong

doi:10.1007/s10532-015-9754-1

Cited by 32 publications

(11 citation statements)

References 27 publications

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“…Figure A shows that neither pallidization nor RDX input changed the community in a major way, e.g., known denitrifying bacteria added up to 88% of the abundance in the biofilm since nitrate was the dominant electron acceptor in the medium. Figure B points out that perchlorate-reducing bacteria Thauera sp.28 , and Azonexus hydrophilus (which also are denitrifiers) accounted for ∼80% of the total abundance in the biofilm and Pd-biofilm. Thauera is possibly linked to the bioreduction of another munition compound, 3-nitro-1,2,3-triazol-5-one (NTO), with acetate as an electron donor .…”

Section: Resultsmentioning

confidence: 99%

Coremoval of Energetics and Oxyanions via the In Situ Coupling of Catalytic and Enzymatic Destructions: A Solution to Ammunition Wastewater Treatment

Zheng

Zhou

Luo

et al. 2022

Environ. Sci. Technol.

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Ammunition wastewater contains toxic nitrated explosives like RDX and oxyanions like nitrate and perchlorate. Its treatment is challenged by low efficiency due to contaminant recalcitrance and high cost due to multiple processes needed for separately removing different contaminant types. This paper reports a H2-based low-energy strategy featuring the treatment of explosives via catalytic denitration followed by microbial mineralization coupled with oxyanion reduction. After a nitrate- and perchlorate-reducing biofilm incapable of RDX biodegradation was coated with palladium nanoparticles (Pd0NPs), RDX was rapidly denitrated with a specific catalytic activity of 8.7 gcat –1 min–1, while biological reductions of nitrate and perchlorate remained efficient. In the subsequent 30-day continuous test, >99% of RDX, nitrate, and perchlorate were coremoved, and their effluent concentrations were below their respective regulation levels. Detected intermediates and shallow metagenome analysis suggest that the intermediates after Pd-catalytic denitration of RDX ultimately were enzymatically utilized by the nitrate- and perchlorate-reducing bacteria as additional electron donor sources.

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Section: Resultsmentioning

confidence: 99%

Coremoval of Energetics and Oxyanions via the In Situ Coupling of Catalytic and Enzymatic Destructions: A Solution to Ammunition Wastewater Treatment

Zheng

Zhou

Luo

et al. 2022

Environ. Sci. Technol.

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“…The nitrate content of the soil is important because nitrate reduction will also occur in perchlorate remediation systems (Srinivasan and Sorial ; Wan et al ). Nitrate reduction often precedes perchlorate reduction, thereby resulting in a perchlorate reduction lag period (Nozawa‐Inoue et al ).…”

Section: Resultsmentioning

confidence: 99%

Biological Reduction of Nitrate and Perchlorate in Soil Microcosms: An Electron Donor Comparison of Glycerol, Emulsified Oil, and Mulch Extract

Sarria¹,

Gonzales²,

Gerrity³

et al. 2018

Groundwater Monitoring Rem

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Diverse technologies have been developed and tested for their efficacy in remediating perchlorate‐contaminated surface water, groundwater, and soil. Biological reduction, particularly when coupled with electron donor augmentation, has been shown to be one of the most cost‐effective alternatives. Numerous electron donors have been evaluated in the literature, but few studies have compared standard vs. slow‐release electron donors for sequential nitrate and perchlorate reduction. This study evaluated the efficacy and kinetics of biological reduction in soil microcosms augmented with emulsified oil (EO), glycerol, and mulch extract. Results indicated that EO and glycerol spiked at approximately 100 times stoichiometric excess (i.e., 100x) achieved similar overall reductions and degradation rates for nitrate and perchlorate, although nitrate appeared to exhibit zero order kinetics while perchlorate exhibited first order kinetics. The zero order rate constants for nitrate reduction were 3.32 mg/L d and 2.57 mg/L d for EO and glycerol, respectively. The first order rate constant for perchlorate reduction was 0.36 day−1 for both EO and glycerol. Stable chemical oxygen demand (COD) concentrations also highlighted the slow‐release properties of EO, which would reduce electron donor consumption in comparison to soluble substrates in soil remediation applications. The microcosms augmented with mulch extract failed to demonstrate any nitrate or perchlorate reduction due to the extract's lower COD concentration. Augmentation with compost or additional processing (i.e., concentration) would be necessary to make the extract a viable alternative.

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“…Two fractions, corresponding to the 13 C (heavy) and 12 C (light), respectively, present in DNA from each sample were selected for high-throughput sequencing. The V3–V4 hypervariable region of bacterial 16S rRNA genes was amplified with the universal primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) 65 . Sequencing was carried out using an Illumina MiSeq platform at the Chinese National Human Genome Center (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Differential incorporation of one-carbon substrates among microbial populations identified by stable isotope probing from the estuary to South China Sea

Deng

Peng

Jiao

et al. 2018

Sci Rep

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Methanol (MOH) and monomethylamine (MMA) are two typical one-carbon (C1) compounds found in natural environments. They play an important role in marine and atmospheric chemistry, cloud formation, and global climate. The main biological sink of MOH and MMA is rapid consumption by marine microbes. Here, field-based time-series incubations with supplemental 13C-labelled MOH and MMA and isotope ratio analyses were performed. A substantial difference in the MOH and MMA incorporation rates and bacterial taxa were observed between the South China Sea (SCS) and the Pearl River estuary. C1 substrates were assimilated more quickly in the estuary than the SCS shelf where MOH and MMA had similar bio-availability. However, microbial responses to MMA may be faster than to MOH in the coastal and basin surface water of the SCS despite similar active bacterial populations. Three ecological types of bacteria, in terms of response to supplemented MOH and MMA, were identified: rapid incorporation (I, dominant C1-incorporating group), slow incorporation (II, minor C1-incorporating group), and no incorporation (III, C1-non-incorporating group). Members of the families Methylophilaceae (β-Proteobacteria) and Piscirickettsiaceae (γ-Proteobacteria) belonged to type I and actively incorporated substrates in the estuary and SCS, respectively. Diverse MOH and MMA-incorporating type II bacteria were identified by stable isotope probing in the SCS, and could play a more important role in the transformation of C1 compounds in marine environments than hitherto assumed.

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Perchlorate reduction by hydrogen autotrophic bacteria and microbial community analysis using high-throughput sequencing

Cited by 32 publications

References 27 publications

Coremoval of Energetics and Oxyanions via the In Situ Coupling of Catalytic and Enzymatic Destructions: A Solution to Ammunition Wastewater Treatment

Coremoval of Energetics and Oxyanions via the In Situ Coupling of Catalytic and Enzymatic Destructions: A Solution to Ammunition Wastewater Treatment

Biological Reduction of Nitrate and Perchlorate in Soil Microcosms: An Electron Donor Comparison of Glycerol, Emulsified Oil, and Mulch Extract

Differential incorporation of one-carbon substrates among microbial populations identified by stable isotope probing from the estuary to South China Sea

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