Percutaneous application of allogeneic adipose-derived mesenchymal stem cell in dogs submitted to minimally invasive plate osteosynthesis of the tibia

Abstract

Purpose To evaluate clinical outcome following minimally invasive plate osteosynthesis (MIPO) associated with percutaneous transplantation of allogeneic adipose-derived mesenchymal stem cells (AD-MSC) at the tibial fracture site in dogs. Methods Thirty-six dogs presenting with nonarticular complete tibial fracture were included in this study. All fractures were treated by the same MIPO technique. The animals were divided in group 1 (n = 20) received a percutaneous application of 3 × 10 6 AD-MSC at the fracture site and group 2 (n = 16) did not receive any adjuvant treatment. Postoperative radiographic examinations were made at 15, 30, 60, 90 and 120 days. Results Fifty-eight percent of the patients were classified as skeletally immature. The median weight of the animals was 18.8 kg. The mean radiographic union time differed statistically between the AD-MSC group (28.5 days) and the control group (70.3 days). Sixty percent of dogs in group 1 and 56.25% of the group 2 were considered immature. Conclusions The use of allogeneic AD-MSC cell therapy and MIPO is a safe, viable and effective technique for promoting bone healing in nonarticular tibial fractures in dogs.

Effects of Autologous Microfragmented Adipose Tissue on Bone Healing: A Blinded, Prospective, Randomised Canine Clinical Trial

“…The T1 results agree with a similar study evaluating the effect of ADSCs on bone healing in dogs undergoing tibial tuberosity advancement surgery; however, the authors did not show significant differences between the treated group and the control group in the subsequent follow-up (60 days) [80]. Franco et al [81] reported that the use of minimally invasive plate osteosynthesis (MIPO) in association with ADSC administration stimulated bone healing after a tibia fracture. They had promising results because, in their study, clinical bone healing occurred on average 28.5 and 70.3 days after treatment, respectively, in the stem cell and control groups.…”

Effects of Autologous Microfragmented Adipose Tissue on Healing of Tibial Plateau Levelling Osteotomies in Dogs: A Prospective Clinical Trial

“…The T1 results agree with a similar study evaluating the effect of ADSCs on bone healing in dogs undergoing tibial tuberosity advancement surgery; however, the results did not show significant differences between the treated group and the control group in the subsequent follow-up (at 60 days) [ 83 ]. Franco et al [ 84 ] reported that the use of minimally invasive plate osteosynthesis (MIPO) in association with ADSC administration stimulated bone healing after a tibial fracture. They reported promising results: in their study, clinical bone healing occurred on average 28.5 and 70.3 days after treatment, respectively, in the stem cell and control groups.…”

Comprehensive assessment of goat adipose tissue-derived mesenchymal stem cells cultured in different media

“…Adipose tissue-derived mesenchymal stem cells (ADSCs) exhibit high expansion potential in vitro, making them useful in stem-cell-based therapies and tissue engineering 7 – 9 . ADSCs have been reported to treat musculoskeletal disorders which do not respond to conventional treatment 10 , such as osteoarthritis 11 , 12 , injuries of the tendo-ligamentous apparatus 13 , 14 , and bone fractures 15 , 16 in companion and large animals.…”